Development and evaluation of inocula combating high acetate concentrations during the start-up of an anaerobic digestion

Lins, Philipp; Reitschuler, Christoph; Illmer, Paul

doi:10.1016/j.biortech.2012.01.145

Cited by 29 publications

(14 citation statements)

References 34 publications

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“…It is also important that the relative change of the parameter following a perturbation is significant compared to background fluctuations and analysis uncertainties. As a matter of fact, the accumulation of both forms of n-butyric acid (HBu) has also been used as an indicator of anaerobic digester failure (Lins et al 2012). However, the experimental results from Lin et al (1998) indicated that the normal-and iso-HBu-degradation activities of methanogens increased by 14-25 % and 17-43 %, respectively, for each gram of the biomass spiked with 0.02-3.89 mg of Mn, Zn, Ni, Fe and Cu.…”

Section: Stress Indicators In Anaerobic Processesmentioning

confidence: 99%

Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass

Mudhoo

Kumar

2013

Int. J. Environ. Sci. Technol.

219

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Heavy metals affect the biochemical reactions that take place during anaerobic digestion processes of organic matter. In this review, the different effects observed in anaerobic digestion processes and during the production of biomethane and biohydrogen from several substrates contaminated with and/or inheriting heavy metals from the substrates themselves were discussed. It has been found that heavy metals exert important roles in biochemical reactions. Heavy metals like copper, nickel, zinc, cadmium, chromium and lead have been overwhelmingly reported to be inhibitory and under certain conditions toxic in biochemical reactions depending on their concentrations. Heavy metals like iron may also exhibit stimulatory effects, but these effects have been scantily observed. This review also concludes that the severity of heavy metal inhibition depends upon factors like metal concentration in a soluble, ionic form in the solution, type of metal species, and amount and distribution of biomass in the digester or chain of biochemical reactions which constitute the anaerobic digestion process. A majority of studies have demonstrated that the toxic effect of heavy metals like chromium, cadmium and nickel is attributable to a disruption of enzyme function and structure by binding of the metal ions with thiol and other groups on protein molecules or by replacing naturally occurring metals in enzyme prosthetic groups. This review has not found published data on the effects of heavy metals on the hydrolysis stage of anaerobic digestion process chemistry, and hence further studies are required to depict any changes.

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Section: Stress Indicators In Anaerobic Processesmentioning

confidence: 99%

Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass

Mudhoo

Kumar

2013

Int. J. Environ. Sci. Technol.

219

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“…Two other clones, DBA-2 (Methanospirillum hungatei JF-1 CP000254.1) and DAA-4 (Methanospirillum hungatei JF-1 CP000254.1), attributed to Methanomicrobiales, colonized the DS. The authors of previous studies have reported that when Methanomicrobiale species are abundant in the system they protect the reactor from external environment shocks, and promote successful operation and stability (De Vrieze et al, 2012;Lins et al, 2012). From the phylogenetic trees, we found that Methanosaeta was dominant in both SS and DS.…”

Section: Clone Library Analysis Of Archaeal Communitymentioning

confidence: 72%

Effect of domestication on microorganism diversity and anaerobic digestion of food waste

Hong²,

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. To accomplish the rapid start-up and stable operation of biogas digesters, an efficient inoculum is required. To obtain such an inoculum for food waste anaerobic digestion, we domesticated dairy manure anaerobic digestion residue by adding food waste every day. After 36 days, the pH and biogas yield stabilized signifying the completion of domestication. During domestication, the microbial communities in the inocula were investigated by constructing 16S rDNA clone libraries. We evaluated the effect of the domesticated inoculum by testing batch food waste anaerobic digestion with a non-domesticated inoculum as a control. The pH and methane yield of the digestion systems were determined as measurement indices. Domestication changed the composition and proportion of bacteria and archaea in the inocula. Of the bacteria, Clostridia (49.3%), Bacteroidales (19.5%), and Anaerolinaceae (8.1%) species were dominant in the seed sludge; Anaerolinaceae (49.0%), Clostridia (28.4%), and Bacteroidales (9.1%), in domestication sludge. Methanosaeta was the dominant genus in both of the seed (94.3%) and domestication (74.3%) sludge. However, the diversity of methanogenic archaea was higher in the domestication than in seed sludge. Methanoculleus, which was absent from the seed sludge, appeared in the domestication sludge (21.7%). When the domesticated inoculum was used, the digestion system worked stably (organic loading rate: 20 gVS/L; methane yield: 292.2 ± 9.8 mL/gVS; VS = volatile solids), whereas the digestion system inoculated with seed sludge failed to generate biogas. The results indicate that inoculum domestication ensures efficient and stable anaerobic digestion by enriching the methanogenic strains.

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“…Several starting-up protocols under stress operations have been established and evaluated. Among them, the optimized method based on the understanding of production mechanism is a better choice [25].…”

Section: Semi-continuous Culture With Modified Starting-up Protocolmentioning

confidence: 99%

Enhancement of rice vinegar production by modified semi-continuous culture based on analysis of enzymatic kinetic

Xia

Zhu

Yang

et al. 2015

Eur Food Res Technol

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Development and evaluation of inocula combating high acetate concentrations during the start-up of an anaerobic digestion

Cited by 29 publications

References 34 publications

Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass

Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass

Effect of domestication on microorganism diversity and anaerobic digestion of food waste

Enhancement of rice vinegar production by modified semi-continuous culture based on analysis of enzymatic kinetic

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