Development and evaluation of an enriched natural antioxidant preparation obtained from aqueous spinach (Spinacia oleracea) extracts by an adsorption procedure

Aehle, Elke; Grandic, Sophie Raynaud-Le; Ralainirina, Robert; Baltora-Rosset, Sylvie; Mesnard, François; Prouillet, Christophe; Mazière, Jean‐Claude; Fliniaux, Marc‐André

doi:10.1016/j.foodchem.2003.10.006

Cited by 74 publications

(54 citation statements)

References 18 publications

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“…More popular than kale, an edible and flowering green vegetables is spinach (Spinacia oleracea) (Wright, 2001). Leaves of this plant contain antioxidant flavonoids, in particular spinacetin and patuletin (Aehle et al, 2004). The biological activities of spinach polyphenols have been already reported (Edenharder et al, 2001;Gil et al, 1999;Lomnitski et al, 2000).…”

Section: Introductionmentioning

confidence: 95%

Effect of Kale Cultivation Conditions on Biosynthesis of Xanthophylls

Ligor¹,

Buszewski²

2012

JFR

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Conditions of plant cultivation directly influence the chlorophyll and carotenoid pigments concentrations. The aim of investigations was the determination of lutein in kale (Brassica oleracea L. var. acephala) upon various cultivation conditions including: low and high temperature (overnight and daytime in the greenhouse the temperature was 12 ± 2°C and in the laboratory the temperature was 22 ± 2°C), lack of water (some plants were watered only one time weekly), saline stress (5% of sodium chloride added to irrigation water; watering two times weekly was applied), as well as UV radiation of plants by means of UV lamp (λ = 254 nm; suitable to UVC solar radiation; duration 4 h per day). Three months old cuttings were taken and next mentioned cultivation conditions were applied during one month. Moreover, the outdoor cultivation of kale cuttings was performed. Qualitative and quantitative analyses of plant extracts were made by use of HPLC-UV-VIS-MS technique. Mean values for lutein accumulation as a function of dry mass (mg/g) in leaves of kale have been presented. Obtained results show maximum xantophylls (lutein) concentration occur in fully developed plant leaves of kale for red kale 2.04 ± 0.15 and green kale 1.10 ± 0.09 mg/g respectively.

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Section: Introductionmentioning

confidence: 95%

Effect of Kale Cultivation Conditions on Biosynthesis of Xanthophylls

Ligor¹,

Buszewski²

2012

JFR

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“…Usually, reversed phase SPE column is used to remove plant impurities such as sugars, proteins and metals prior to analyses, in order to prevent matrix interference and improve data reliability. [7][8][9] Previous results indicated that rutin could have the highest adsorption capacity in C18 reversed phase. 10 This long organosilyl ligand phase has higher carbon percentage and lower polarity in chemically bonded packing materials than C8 and C4.…”

Section: Introductionmentioning

confidence: 99%

Recovery of Rutin from Labisia pumila Extract Using Solid Phase Extraction

Chua

Ruzlan

Sarmidi

2017

ACSi

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Reflux extraction was used to prepare crude extract from the leaves of Labisia pumila var. Alata using 60% methanol. The crude extract was subsequently fractionated by C18 solid phase extraction to recover high yield of rutin using 20-100% methanol. The volume of eluent to recover rutin was found to decrease with the increase of methanol concentration. The recovery of rutin was increased from 20 to 80% methanol system, but slightly decreased in the 100% methanol system. Approximately, 70% of rutin could be recovered using the 80% methanol system. This solvent system also appears to have the lowest distance (9.44 MPa 1/2 ) for rutin as estimated by Hansen solubility. The recovered rutin rich fraction could achieve up to 3.96 mg/g of fraction which was about 4-fold increment from the crude extract. The increment was also noticed for its antioxidant capacity expressed as scavenging activity which was 2 times higher than crude extract. A portion of water (20%) in the 80% methanol system is important to improve the yield of rutin. Rutin is a glycosylated flavonol, and therefore a small portion of water could enhance its elution compared to the lower performance of 100% methanol in rutin recovery.

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“…These compounds are water soluble and may occur in combination with a sugar molecule, as glycosides (Harbone, 1998). The most important natural antioxidants commercially exploited include tocopherols and ascorbic acid, which have been successfully extracted from rosemary (Tena and Valcárcel, 1997), green tea (Wang and Halliwell, 2001) and spinach (Aehle et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Extraction of phenolic compounds from Temnocalyx obovatus

Mahamadi¹,

Togarepi²,

Dzomba³

2011

Afr. J. Biotechnol.

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Phenolic compounds yield in plant extracts depend on the method employed in the extraction process. In this study, we investigated systematically, a method of determination of extraction yield of antioxidant compounds from Temnocalyx obovatus. A sample treatment and preparation protocol that employs strict statistical treatment to ensure sample homogeneity was applied. As preliminary indicator of homogeneity, total nitrogen and phosphorus were determined and an iterative process using Levene test was applied to statistically test the homogeneity of the ground T. obovatus leaves. At p = 0.01, homogeneity of the plant material was achieved on the third ground sample. Among the six solvents used, methanol gave the best yield of extractable phytochemicals and a butylated hydroxytoluene-spike recovery of 77%, whereas diethyl ether gave the lowest yield and a butylated hydroxytoluene-spike recovery of 63%, suggesting that optimal yields tended to be favoured by more polar solvents.

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Development and evaluation of an enriched natural antioxidant preparation obtained from aqueous spinach (Spinacia oleracea) extracts by an adsorption procedure

Cited by 74 publications

References 18 publications

Effect of Kale Cultivation Conditions on Biosynthesis of Xanthophylls

Effect of Kale Cultivation Conditions on Biosynthesis of Xanthophylls

Recovery of Rutin from Labisia pumila Extract Using Solid Phase Extraction

Extraction of phenolic compounds from Temnocalyx obovatus

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