Development and evaluation of a Salmonella typhimurium flagellin based chimeric DNA vaccine against infectious bursal disease of poultry

Deb, Rajib; Dey, Sohini; Mohan, C. Madhan; Gaikwad, Satish S.; Kamble, Nitin Uttam; Khulape, Sagar A.; Gupta, Shishir Kumar; Maity, Hemanta Kumar; Pathak, D. C.

doi:10.1016/j.rvsc.2015.07.004

Cited by 14 publications

(6 citation statements)

References 60 publications

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“…Such a strategy will permit production of a vaccine with adequate stability in the aggressive environment of the intestinal tract and the ability to bind M cells to favor induction of the most effective immune responses. The combination with other treatments, including passive delivery of specific monoclonal antibodies (236), DNA vaccination (237) or recombinant probiotic delivery, may turn out to eventually define the optimal system to fight the still-devastating consequences of Salmonella infection worldwide.…”

Section: Resultsmentioning

confidence: 99%

Vaccination against Salmonella Infection: the Mucosal Way

Gayet¹,

Bioley²,

Rochereau³

et al. 2017

Microbiol Mol Biol Rev

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subspecies includes several serovars infecting both humans and other animals and leading to typhoid fever or gastroenteritis. The high prevalence of associated morbidity and mortality, together with an increased emergence of multidrug-resistant strains, is a current global health issue that has prompted the development of vaccination strategies that confer protection against most serovars. Currently available systemic vaccine approaches have major limitations, including a reduced effectiveness in young children and a lack of cross-protection among different strains. Having studied host-pathogen interactions, microbiologists and immunologists argue in favor of topical gastrointestinal administration for improvement in vaccine efficacy. Here, recent advances in this field are summarized, including mechanisms of bacterial uptake at the intestinal epithelium, the assessment of protective host immunity, and improved animal models that closely mimic infection in humans. The pros and cons of existing vaccines are presented, along with recent progress made with novel formulations. Finally, new candidate antigens and their relevance in the refined design of anti- vaccines are discussed, along with antigen vectorization strategies such as nanoparticles or secretory immunoglobulins, with a focus on potentiating mucosal vaccine efficacy.

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Section: Resultsmentioning

confidence: 99%

Vaccination against Salmonella Infection: the Mucosal Way

Gayet¹,

Bioley²,

Rochereau³

et al. 2017

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

show abstract

“…A chimeric DNA vaccine encoding C-terminal HSP70 of Mycobacterium tuberculosis fused with full-length IBDV VP2 gene induced mixed Th1 and Th2 responses31 and completely protected the birds when boosted with SVP-based vaccine in which VP2 has been expressed in S. cerevisiae. 43 A TLR-5 ligand flagellin (fliC) antigen of Salmonella typhimurium when fused with an N-terminal of VP2 and injected as a DNA vaccine produced 80% protection but could stimulate both the arms of immunity 65. For achieving a successful immunization with IBDV-DNA vaccine, age of bird, route of vaccine administration and virulence of challenge virus are crucial.…”

Section: One-step Strip Testmentioning

confidence: 99%

<p>Infectious bursal disease virus in chickens: prevalence, impact, and management strategies</p>

Dey¹,

Pathak²,

Narayan³

et al. 2019

VMRR

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Infectious bursal disease (IBD), also known as Gumboro disease, is a highly contagious, immunosuppressive disease of young chickens. Although first observed about 60 years ago, to date, the disease is responsible for major economic losses in the poultry industry worldwide. IBD virus (IBDV), a double-stranded RNA virus, exists as two serotypes with only serotype 1 causing the disease in young chickens. The virus infects the bursa of Fabricius of particularly the actively dividing and differentiating lymphocytes of the B-cells lineage of immature chickens, resulting in morbidity, mortality, and immunosuppression. Immunosuppression enhances the susceptibility of chickens to other infections and interferes with vaccination against other diseases. Immunization is the most important measure to control IBD; however, rampant usage of live vaccines has resulted in the evolution of new strains. Although the immunosuppression caused by IBDV is more directed toward the B lymphocytes, the protective immunity in birds depends on inducement of both humoral and cell-mediated immune responses. The interference with the inactivated vaccine induced maternally derived antibodies in young chicks has become a hurdle in controlling the disease, thus necessitating the development of newer vaccines with improved efficacy. The present review illustrates the overall dynamics of the virus and the disease, and the recent developments in the field of virus diagnosis and vaccine research.

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“…Figure 5B shows that about 92 % of the amino acid residues were in the most favored regions and only 2 % were in the disallowed regions. Due to the lack of appropriate crystal structure in the (Glycine 4 Serine) 3 linker, the unfavorable residues were mainly located in this region of the model. These results indicated that the scFv model was suitable for the molecular docking study.…”

Section: Homology Model Building and Validationmentioning

confidence: 99%

“…Gumboro has no treatments or drugs and therefore, it is important to try to prevent the disease by observing strict hygienic and vaccination programs [3]. Nevertheless, application of vitamins as well as antibiotics can prevent subsequence infections [4,5].…”

Section: Introductionmentioning

confidence: 99%