Development and Evaluation of a Rotary Cell for Capillary Electrophoresis−Chemiluminescence Detection

Abstract

Many efforts have been made toward the advancement of capillary electrophoresis chemiluminescence (CE-CL) detection and its applications through continuous development and improvement of interfaces. In this study, a novel rotary cell for CE-CL detection was fabricated and evaluated. A ring-shaped narrow channel with a quartz bottom is made in a cell body to hold CL reactants and act as the reaction chamber. The CE capillary is placed closely to the bottom of the reaction chamber where analyte is deposited into the CL reactants for reactions to occur. Detection is achieved with a photomultiplier tube below the reaction channel. An advantage of the rotary reaction cell is that it renews the reactants at the capillary end as it revolves at a preset speed during experiments. The rotary detection cell presents a new concept to the conventional coaxial-flow configuration by solving the problems of bubble formation and flow blockage that often interrupt the electrophoresis. Two standard proteins, horseradish peroxidase (HRP) and hemoglobin (Hb), were used to evaluate the interface's performance with luminol/H(2)O(2) CL system. Satisfactory sensitivities (LOD of 0.91 x 10(-9) M for HRP, and 4.37 x 10(-8) M for Hb at S/N = 3) were obtained in this proof-of-concept experiment. This novel design provides a straightforward and robust interface for CE-CL hyphenation.

Ultrasensitive determination of epicatechin, rutin, and quercetin by capillary electrophoresis chemiluminescence

“…In the CE-CL instrumental configuration, for achieving good separation efficiency and sensitivity, the design of CE-CL interface was crucial [28], and many efforts were achieved in this aspect [29,30]. Another key factor was the matching of flow speed between the separation buffer and CL reagent, as well as the mode of the liquid from the reaction capillary into the waste reservoir.…”

Recent advances in chemiluminescence detection coupled with capillary electrophoresis and microchip capillary electrophoresis

“…1, Step motor and lead screw; 2, detection cell body; 3, Pt cathode; 4, slit; 5, quartz bottom; 6, PMT; 7, separation capillary; 8, offset between capillary and the slit window. Source: Reprinted with permission from . Copyright (2010) American Chemical Society.…”

Determination of alkaloids in Sinomenium acutum by field‐amplified sample stacking in capillary electrophoresis with chemiluminescene detection

“…Compared to UV detection, chemiluminescence (CL) detection is an alternative ultrasensitive detection method for its high sensitivity, wide linear range, low cost and no light source. The coupling of CE with CL detection that combines the high separation efficiency of CE with the high sensitivity of CL has proven an attractive detection technique for ultrasensitive analysis, and used in pharmaceutical, clinical and food analysis . CE with electrochemiluminescence detection has been developed to determine sinomenine in Sinomenium acutum .…”