Development and evaluation of a polymerase chain reaction assay to detect Bovine herpesvirus 1

Medina, Majela Rodríguez; Sánchez, Mislay Avila; Landa, H. Díaz de Arce; Valle, Maritza Barrera

doi:10.5424/sjar/2009071-398

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“…All the samples were collected from the suspected animals showing clinical signs of IBR. Shedding of virus in secretion is more during the clinical phase of disease which increases the sensitivity of virus isolation and PCR [23,24]. …”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1.

et al. 2016

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Aim:This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease.Materials and Methods:Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software.Results:A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain.Conclusion:BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain.

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Section: Discussionmentioning

confidence: 99%