Development and evaluation of a subunit vaccine for infectious bovine rhinotracheitis

Lupton, H W

doi:10.31274/rtd-180814-5437

Cited by 7 publications

(10 citation statements)

References 7 publications

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“…When maximal RIP activity was observed, the VN titers were also elevated. VN titers reached levels similar to those found by others carrying out experimental infections (12) or after vaccination with modified live viruses (9,17,25). However, it was not possible to correlate which polypeptides were recognized in the ELISA or in the VN tests because the RIP assay detected activity to so many polypeptides.…”

Section: Discussionsupporting

confidence: 87%

“…The specifity of the immune response necessary for resistance or recovery from bovine herpes virus type 1 (BHV-1) is not well defined. Although live virus vaccines have been prepared and shown to induce some level of protective immunity (9,12,17,25), the protective functions and specificities are not known. Serum antibody titers to the virus do not assure that animals are protected; outbreaks occur in the presence of such titers (13,16).…”

mentioning

confidence: 99%

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Immune response to bovine herpes herpesvirus type 1 infections: virus-specific antibodies in sera from infected animals

Collins¹,

Butcher²,

Riegel³

1985

J Clin Microbiol

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The virus specificity of antibodies against bovine herpes virus type 1 was determined with a radioimmunoprecipitation assay and serum collected from natural and experimentally induced infections. By using sequentially collected sera, the development of antibodies to 4 to 5 viral glycoproteins and 11 to 12 nonglycosylated proteins was followed for the first 50 days after infection. The major and most consistent responses in experimentally and naturally infected animals were to four glycoproteins with molecular weights of 102,000, 96,000, 69,000, and 55,000, as well as to a major virion 115,000-molecular-weight nonglycosylated protein. The four glycoproteins were all coprecipitated by a neutralizing monoclonal antibody and were probably involved as target antigens in virus neutralization. Another antigenically unrelated glycoprotein with a molecular weight of 82,000 and a nonglycosylated protein with a molecular weight of 91,000 were also precipitated, but the immune response to these two proteins was transient. Reactivity to gp82 was only weakly detected in serum from naturally infected animals. Contact control animals which did not contract a bovine herpes virus type 1 infection but were exposed to infected animals with signs of severe illness had antibodies which recognized gplO2, gp96, gp69 and gp55 as well as pli5. These antibodies were present in low amounts and, in contrast to infected animals, did not increase between acute and convalescent sampling.

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Section: Discussionsupporting

confidence: 87%

mentioning

confidence: 99%

Immune response to bovine herpes herpesvirus type 1 infections: virus-specific antibodies in sera from infected animals

Collins¹,

Butcher²,

Riegel³

1985

J Clin Microbiol

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“…BHV-1 glycoproteins have been reported to induce virusneutralizing antibodies (6,20,24). The association of two glycoproteins with the BHV-1 envelope has been described previously (2).…”

mentioning

confidence: 92%

“…Up to 33 structural polypeptides, 11 of which are glycosylated, have been described for BHV-1 (22). The glycoproteins in the viral envelope are pivotal in the host-virus relationship since they are involved in the recognition, attachment, and penetration of herpesviruses into susceptible cells (18), in viral neutralization (10,20), and in the immune destruction of infected cells (1,3,23).…”

mentioning

confidence: 99%

Characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods

Marshall¹,

Rodrı́guez²,

Letchworth³

1986

J Virol

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and 45,000 (designated as 180K, 150K, etc.) and a single nonglycosylated 107,000-molecularweight (107K) protein were quantitatively removed from purified bovine herpesvirus 1 (BHV-1) virions by detergent treatment. Immunoprecipitations with monospecific and monoclonal antibodies showed that three sets of coprecipitating glycoproteins, 180K/97K, 150K/77K, and 130K/74K/55K, were the major components of the BHV-1 envelope. These glycoproteins were present in the envelope of the virion and on the surface of BHV-1-infected cells and reacted with neutralizing monoclonal and monospecific antibodies. Antibodies to 150K/77K protein had the largest proportion of virus-neutralizing antibodies, followed by antibodies to 180K/97K protein. Monoclonal antibodies to 130K/74K/55K protein were neutralizing but only in the presence of complement; however, monospecific antisera produced with 55K protein did not have neutralizing activity. Analysis under nonreducing conditions showed that the 74K and 55K proteins interact through disulfide bonds to form the 130K molecule. Partial proteolysis studies showed that the 180K protein was a dimeric form of the 97K protein and that the 150K protein was a dimer of the 77K protein, but these dimers were not linked by disulfide bonds. The 107K protein was not glycosylated and induced antibodies that did not neutralize BHV-1. The 64K protein was not precipitated by anti-BHV-1 convalescent antisera, and monospecific antisera to this protein precipitated several polypeptides from uninfected cell lysates, suggesting that 64K is a protein of cellular origin associated with the BHV-1 virion envelope.

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“…Rabbits have been used in laboratory work, in research and vaccine testing; it proposed to be used in immunogenicity evaluation of inactivated equine herpes virus vaccine (British Pharmacopoeia Veterinary, 2005). Also, many authors succeeded to use the rabbits for evaluation of different types of veterinary and human vaccines (Lupton and Reed, 1980, Tsenova et al, 2006and Ahmad et al, 2012. The specific antibody response to vaccination with inactivated viral vaccine has been considered a major indicator of such vaccine potency (Fulton et al, 1995 andKerkhofs et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Utility of laboratory animals for relative potency evaluation of bovine respiratory viral vaccine

Sayed

Mostafa

Shafik

2016

Benha Veterinary Medical Journal

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Development and evaluation of a subunit vaccine for infectious bovine rhinotracheitis

Cited by 7 publications

References 7 publications

Immune response to bovine herpes herpesvirus type 1 infections: virus-specific antibodies in sera from infected animals

Immune response to bovine herpes herpesvirus type 1 infections: virus-specific antibodies in sera from infected animals

Characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods

Utility of laboratory animals for relative potency evaluation of bovine respiratory viral vaccine

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