Development and evaluation of a gold nanoparticle based Lateral Flow assay (LFA) strip test for detection of Brucella spp.

Prakash, Chandan; Kumar, B. Mohan; Singh, Rabindra Prasad; Singh, Praveen; Shrinet, Garima; Das, Abhishek; Ashmi, Marcia; Abhishek, Abhishek; Singh, Mithilesh; Gupta, Vivek Kumar

doi:10.1016/j.mimet.2021.106185

Cited by 12 publications

(12 citation statements)

References 17 publications

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“…The fifth route is based on NP accumulation reactions [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ]. This strategy has been generally used in flat substrate-based systems, such as the lateral flow assay (LFA), µ-paper-based analytical device (µ-PAD), and paper-based dipstick system [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ]. These systems rely on the capillary action of fluids and colored NPs.…”

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

“…The concentrated NPs act as colorimetric labels or color generation factors. Colored NPs used for colorimetric detection include AuNPs, silver NPs (AgNPs), and colloidal carbon [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ]. Red AuNPs are one of the widely used colorimetric labels because of their bright color, high stability, and high biocompatibility [ 80 ].…”

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

“…The representative colored NPs widely used in this approach are colloidal AuNPs. In previous studies, AuNPs were generally applied to paper-based flow assay strips [ 74 , 75 , 76 , 111 ]. The concentrated NPs acted as colorimetric labels and generated color in the test zone.…”

Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

“…For example, to detect Brucella species, an LFA strip was fabricated in laminates consisting of a nitrocellulose (NC) membrane, conjugation pad, sample pad, and absorption pad. One anti- Brucella antibody was deposited on the control and test lines of the strip, and another anti- Brucella antibody conjugated with AuNP (Ab-AuNP) was deposited on the conjugate pad [ 74 ]. When a sample containing the target bacteria was deposited on the sample pad, the sample bacterial suspension bound to Ab-AuNPs during flow through the strip.…”

Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

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Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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Bacterial contamination is a public health concern worldwide causing enormous social and economic losses. For early diagnosis and adequate management to prevent or treat pathogen-related illnesses, extensive effort has been put into the development of pathogenic bacterial detection systems. Colorimetric sensing systems have attracted increasing attention due to their simple and single-site operation, rapid signal readout with the naked eye, ability to operate without external instruments, portability, compact design, and low cost. In this article, recent trends and advances in colorimetric systems for the detection and monitoring of bacterial contamination are reviewed. This article focuses on pathogen detection strategies and technologies based on reaction factors that affect the color change for visual readout. Reactions used in each strategy are introduced by dividing them into the following five categories: external pH change-induced pH indicator reactions, intracellular enzyme-catalyzed chromogenic reactions, enzyme-like nanoparticle (NP)-catalyzed substrate reactions, NP aggregation-based reactions, and NP accumulation-based reactions. Some recently developed colorimetric systems are introduced, and their challenges and strategies to improve the sensing performance are discussed.

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Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

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Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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“…The resulting complexes interact with the antigen immobilized in the test zone (TZ) of the strip, whereas unbound compounds move across this zone. Therefore, the coloration of the TZ indicates the presence of specific antibodies in the tested sample [ 8 , 17 , 18 , 19 ]. However, non-specific immunoglobulins block binding sites at the surface of nanoparticles and only a minor part of specific antibodies can form labeled complexes in the TZ.…”

Section: Introductionmentioning

confidence: 99%

Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes

et al. 2022

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The presence of pathogen-specific antibodies in the blood is widely controlled by a serodiagnostic technique based on the lateral flow immunoassay (LFIA). However, its common one-stage format with an antigen immobilized in the binding zone of a test strip and a nanodispersed label conjugated with immunoglobulin-binding proteins is associated with risks of very low analytical signals. In this study, the first stage of the immunochromatographic serodiagnosis was carried out in its traditional format using a conjugate of gold nanoparticles with staphylococcal immunoglobulin-binding protein A and an antigen immobilized on a working membrane. At the second stage, a labeled immunoglobulin-binding protein was added, which enhanced the coloration of the bound immune complexes. The use of two separated steps, binding of specific antibodies, and further coloration of the formed complexes, allowed for a significant reduction of the influence of non-specific immunoglobulins on the assay results. The proposed approach was applied for the serodiagnosis using a recombinant RBD protein of SARS-CoV-2. As a result, an increase in the intensity of test zone coloration by more than two orders of magnitude was demonstrated, which enabled the significant reduction of false-negative results. The diagnostic sensitivity of the LFIA was 62.5% for the common format and 100% for the enhanced format. Moreover, the diagnostic specificity of both variants was 100%.

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A fluorescence-based immunochromatographic assay using quantum dot-encapsulated nanoparticles for the rapid and sensitive detection of fetuin-B

Hou,

Cao,

Deng

et al. 2024

Analytica Chimica Acta

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Development and evaluation of a gold nanoparticle based Lateral Flow assay (LFA) strip test for detection of Brucella spp.

Cited by 12 publications

References 17 publications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes

A fluorescence-based immunochromatographic assay using quantum dot-encapsulated nanoparticles for the rapid and sensitive detection of fetuin-B

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