Development and evaluation of a rapid IgM capture ELISA (JEV-Chex) for the diagnosis of Japanese encephalitis

Ravi, Vasanthapuram; Desai, Anita; Balaji., M; Apte, M. P.; Lakshman, Latha; Dk, Subbakrishna; Sridharan, Gopalan; Dhole, Tapan N.; Ravikumar, B. V.

doi:10.1016/j.jcv.2005.11.004

Cited by 39 publications

(29 citation statements)

References 10 publications

(14 reference statements)

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“…6,9 The XCyton kit exhibited a higher sensitivity (90%) and specificity (97.5%) compared with the Panbio kit ( Table 3 ). However, the differences were not significant at the 95% confidence level.…”

Section: Discussionmentioning

confidence: 99%

“…All samples were initially characterized at NIMHANS by an in-house JEV MAC-ELISA, 9 with a subset tested by a herpes simplex IgG ELISA, 10 and a measles IgG ELISA. 11 The samples were numerically coded at NIMHANS by one of the investigators (AD), and the personnel testing the samples in this evaluation were blind to the codes ( Table 1 ).…”

Section: Samplesmentioning

confidence: 99%

“…It was performed according to the kit instructions wherein CSF samples were tested at a 1:10 dilution as described. 9 Samples with JEV CheX ELISA units < 30 are interpreted as negative, 30-99 are classified as suspected recent flavivirus infection, and ≥ 100 are considered positive for JEV infection. Statistical methods.…”

Section: Samplesmentioning

confidence: 99%

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Evaluation of IgM Antibody Capture Enzyme-Linked Immunosorbent Assay Kits for Detection of IgM against Japanese Encephalitis Virus in Cerebrospinal Fluid Samples

Ravi

Robinson²,

Russell³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. Infection with Japanese encephalitis virus (JEV) is a major public health problem in Asia. Detection of JEVspecific IgM in serum and cerebrospinal fluid (CSF) by the IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) is currently the most widely used diagnostic method to detect JEV infection. Because of the possible presence of IgM cross-reactivity with other flaviviruses in serum and the high ratio of inapparent-to-apparent JEV infections, a positive result in serum only suggests a recent infection and not necessarily an encephalitic illness caused by JEV. Consequently, detection of JEV-specific IgM in CSF assumes great diagnostic relevance. We evaluated two commercial JEV MAC-ELISA kits using 60 CSF samples obtained from patients with acute encephalitis syndrome. The Panbio and XCyton kits had sensitivities of 65-80% and 95% and specificities of 90% and 97.5%, respectively. Performance information on these commercial JEV MAC-ELISA kits for CSF should assist in laboratory-based JE surveillance programs.

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Section: Discussionmentioning

confidence: 99%

Section: Samplesmentioning

confidence: 99%

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Evaluation of IgM Antibody Capture Enzyme-Linked Immunosorbent Assay Kits for Detection of IgM against Japanese Encephalitis Virus in Cerebrospinal Fluid Samples

Ravi

Robinson²,

Russell³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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“…74 There are three kits available commercially -the Excyton kit developed by the National Institute of Mental Health and Neurosciences, (Bangalore, India), the Inbios kit for JE (InBios International Inc., Seattle, Washington, USA) and a combo kit for dengue and JE marketed by PanBio, Brisbane, Australia. [75][76][77] A comparative study of these kits revealed that sensitivity of all three was comparable but the PanBio kit had the highest specificity. Specificity of the Excyton kit was higher if dengue was excluded or only encephalitis cases were tested.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

Prevention, diagnosis, and management of Japanese encephalitis in children

Kumar¹

2014

PHMT

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Japanese encephalitis is the single largest cause of viral encephalitis in the world today. It is caused by a Flavivirus whose natural cycle occurs in mosquito and vertebrate hosts (ardeid birds and pigs) and man is an incidental dead-end host. It tends to occur in outbreaks in poor rural regions of Asia where rice growing and pig rearing are a way of life. The illness has three stages -a prodromal stage with fever, headache, vomiting, and other nonspecific symptoms, an acute encephalitic stage with convulsions, coma, and signs of raised intracranial tension, and a convalescent stage. Differential diagnosis is very wide and even during epidemics it can be mimicked by many infectious and noninfectious disorders. The mainstay of laboratory diagnosis is the antibody capture enzyme-linked immunosorbent assay technique in cerebrospinal fluid. Treatment is essentially supportive and no antiviral has yet proven effective in randomized controlled trials. The mainstay of prevention is by vaccination. Many effective and safe vaccines are available and the IXIARO ® vaccine -an inactivated vaccine from the SA-14-14-2 strain grown in vero cells -has received US Food and Drugs Administration approval. Japanese encephalitis control is thus a global health priority.

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“…ELISA is the preferable method to detect JEV antibodies, because it does not require live JEV but only a small amount of serum; additionally, the procedure is simple [18]. Several ELISA kits specific for the JEV antigen or antibody (antigen capture ELISA, immunoglobulin [Ig]M capture ELISA, and IgG ELISA) have been commercialized in the human field, but commercially available ELISA kits in the veterinary field, especially those for pigs, are rare [12,15].…”

Section: Introductionmentioning

confidence: 99%

Improvement of indirect enzyme-linked immunosorbent assay for detection of Japanese encephalitis virus antibodies in swine sera

Yang¹,

Kim²,

Jo³

et al. 2017

Korean Journal of Veterinary Research

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Japanese encephalitis (JE) is an important zoonosis caused by the mosquito-transmitted JE virus (JEV), which is a causative agent of reproductive failure in pregnant sows. Detection of JEV antibodies in swine is performed by hemagglutination inhibition (HI), virus neutralization (VN), and the plaque reduction neutralization test (PRNT). The most stringent PRNT is the 90% endpoint PRNT (PRNT 90 ). These conventional assays are difficult to carry out in diagnostic laboratories with insufficient instruments or cell culture systems. An alternative assay that is easily conducted and time efficient is required. In this study, we improved the indirect enzyme-linked immunosorbent assay (I-ELISA) with clarified antigen for the detection of JEV antibodies. The I-ELISA results obtained from 175 swine serum samples were compared with HI, VN, and PRNT 90 results. The sensitivity of I-ELISA was 91.8%, 95.0%, and 94.7% compared with HI, VN, and PRNT 90 results, respectively. The specificity of I-ELISA was 92.2%, 94.7%, and 94.7% compared with HI, VN, and PRNT 90 results, respectively. Moreover, the I-ELISA results were significantly correlated with the HI (r = 0.93), VN (r = 0.95), and PRNT 90 (r = 0.92) results. These results suggest that the improved I-ELISA is useful for serosurveillance of JEV in swine.

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Development and evaluation of a rapid IgM capture ELISA (JEV-Chex) for the diagnosis of Japanese encephalitis

Cited by 39 publications

References 10 publications

Evaluation of IgM Antibody Capture Enzyme-Linked Immunosorbent Assay Kits for Detection of IgM against Japanese Encephalitis Virus in Cerebrospinal Fluid Samples

Evaluation of IgM Antibody Capture Enzyme-Linked Immunosorbent Assay Kits for Detection of IgM against Japanese Encephalitis Virus in Cerebrospinal Fluid Samples

Prevention, diagnosis, and management of Japanese encephalitis in children

Improvement of indirect enzyme-linked immunosorbent assay for detection of Japanese encephalitis virus antibodies in swine sera

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