Development and evaluation of a broth macrodilution method to determine the biocide susceptibility of bacteria

Feßler, Andrea T.; Schug, Angela R.; Geber, Franziska; Scholtzek, Anissa D.; Merle, Roswitha; Brombach, Julian; Hensel, Vivian; Meurer, Marita; Michael, Geovana Brenner; Reinhardt, Mario; Speck, Stephanie; Truyen, Uwe; Schwarz, Štefan

doi:10.1016/j.vetmic.2018.07.006

Cited by 20 publications

(37 citation statements)

References 20 publications

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“…MIC was determined by the broth macrodilution method to determine the power of antimicrobial activity of the digested tuna towards Gram-positive bacteria [ 29 , 30 ]. Stock solutions of the digested tuna in deionized water were prepared as follows: extracts of C18 (7.0 mg mL −1 A280 1.606) and extracts of GCB (7.0 mg mL −1 A280 2.276) were diluted with tryptic soy broth (TSB Sigma-Aldrich) to working concentrations of 3.5 mg mL −1 , 1 mg mL −1 and 500 mg mL −1 , to obtain a final volume of 1 mL.…”

Section: Methodsmentioning

confidence: 99%

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Cerrato

Capriotti

Capuano

et al. 2020

Foods

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Due to the rapidly increasing resistance to conventional antibiotics, antimicrobial peptides are emerging as promising novel drug candidates. In this study, peptide fragments were obtained from yellowfin tuna muscle by simulated gastrointestinal digestion, and their antimicrobial activity towards Gram-positive and Gram-negative bacteria was investigated. In particular, the antimicrobial activity of both medium- and short-sized peptides was investigated by using two dedicated approaches. Medium-sized peptides were purified by solid phase extraction on C18, while short peptides were purified thanks to a graphitized carbon black sorbent. For medium-sized peptide characterization, a peptidomic strategy based on shotgun proteomics analysis was employed, and identification was achieved by matching protein sequence database by homology, as yellowfin tuna is a non-model organism, leading to the identification of 403 peptides. As for short peptide sequences, an untargeted suspect screening approach was carried out by means of an inclusion list presenting the exact mass to charge ratios (m/z) values for all di-, tri- and tetrapeptides. In total, 572 short sequences were identified thanks to a customized workflow dedicated to short peptide analysis implemented on Compound Discoverer software.

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Section: Methodsmentioning

confidence: 99%

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Cerrato

Capriotti

Capuano

et al. 2020

Foods

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“…The test ranges were as follows: benzalkonium chloride 0.00005–0.0008%, chlorhexidine 0.000025–0.0008%, glutardialdehyde 0.03–1%, and isopropanol 4 to 12%. The results were read after 24 h incubation at 37°C ( Feßler et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Molecular Analysis of Two Different MRSA Clones ST188 and ST3268 From Primates (Macaca spp.) in a United States Primate Center

et al. 2018

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Methicillin-resistant Staphylococcus aureus (MRSA) were identified in macaques, their environmental facility, and nasal cultures of personnel from the Washington National Primate Research Center [WaNPRC] and included MRSA ST188 SCCmec IV and MRSA ST3268 SCCmec V. The aim of the current study was to determine the carriage of virulence genes, antibiotic resistance genes, and other characteristics of the primate MRSA isolates to determine if there were any obvious differences that would account for differences in transmission within the WaNPRC facility. In total, 1,199 samples from primates were tested for the presence of MRSA resulting in 158 MRSA-positive samples. Fifteen ST188 isolates (all from Macaca nemestrina) and nine ST3268 (four from Macaca mulatta, two from Macaca fascicularis, three from M. nemestrina), were selected for further characterization. All but one of the 15 ST188 isolates had spa type t189 and the remaining one had spa type t3887. These isolates were resistant to β-lactams [blaZ, mecA], macrolides/lincosamides [erm(B)], aminoglycosides [aacA-aphD], and fluoroquinolones. Five isolates were additionally resistant to tetracyclines [tet(K)] and had elevated MICs for benzalkonium chloride [qacC]. In comparison, the nine ST3268 isolates had the related spa types t15469 (n = 5) and t13638 (n = 4). All nine ST3268 isolates were resistant to β-lactams [blaZ, mecA], and tetracyclines [tet(K)]. Some isolates were additionally resistant to aminoglycosides [aacA-aphD], fluoroquinolones and/or showed elevated MICs for benzalkonium chloride [qacC]. In contrast to the ST188 isolates, the ST3268 isolates had the enterotoxin gene cluster egc [seg, sei, selm, seln, selo, selu] and enterotoxin genes sec and sel. The two clones have differences regarding their spa types, virulence and antibiotic resistance genes as well as ST and SCCmec types. However, the data presented does not provide insight into why ST188 spreads easily while ST3268 did not spread within the WaNPRC in-house primates.

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“…Moreover, there has been evidence that, once resistant staphylococcal isolates enter a human or veterinary clinic [11,34,35], the number of nosocomial infections—in particular wound infections [34,35]—increases. Nosocomial settings provide a selective pressure to bacterial pathogens since antimicrobial agents and also biocides are widely used [36,37]. While horse clinics have been identified as “hot spots” for pathogens accumulating antibiotic resistance in the recent past, little is known about frequency and dimension of biocide resistance.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, more research is needed in this particular field and biocide susceptibility of field isolates should be monitored. However, studies determining MICs for biocides often lack an approved method and are therefore difficult to compare [37].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility

Scholtzek

Hanke

Walther

et al. 2019

Toxins

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The detection of borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a challenge to both, veterinary and human laboratories. Between 2015 and 2017, 19 equine S. aureus with elevated minimal inhibitory concentrations for oxacillin were detected in routine diagnostics. The aim of this study was to characterize these isolates to identify factors possibly associated with the BORSA phenotype. All S. aureus were subjected to antimicrobial susceptibility testing and whole genome sequencing (WGS). A quantifiable β-lactamase activity assay was performed for a representative subset of 13 isolates. The WGS data analysis of the 19 BORSA isolates identified two different genomic lineages, sequence type (ST) 1 and ST1660. The core genome multilocus sequence typing (cgMLST) revealed a close relatedness of all isolates belonging to either ST1 or ST1660. The WGS analysis identified the resistance genes aadD, dfrG, tet(L), and/or blaZ and aacA-aphD. Phenotypic resistance to penicillins, aminoglycosides, tetracyclines, fluoroquinolones and sulfamethoxazole/trimethoprim was observed in the respective isolates. For the penicillin-binding proteins 1–4, amino acid substitutions were predicted using WGS data. Since neither transglycosylase nor transpeptidase domains were affected, these alterations might not explain the BORSA phenotype. Moreover, β-lactamase activity was found to be associated with an inducible blaZ gene. The lineage-specific differences regarding the expression profiles were noted.

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Development and evaluation of a broth macrodilution method to determine the biocide susceptibility of bacteria

Cited by 20 publications

References 20 publications

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Molecular Analysis of Two Different MRSA Clones ST188 and ST3268 From Primates (Macaca spp.) in a United States Primate Center

Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility

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