Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens

Elf, Sonja; Auvinen, Pauliina; Jahn, Lisa; Liikonen, Karoliina; Sjöblom, Solveig; Saavalainen, Päivi; Mäki, Minna; Eboigbodin, Kevin E.

doi:10.1016/j.diagmicrobio.2018.04.006

Cited by 5 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Viral pathogens are common drivers of acute RTI in both adults and children. PCR-based methods are already commonplace in diagnostic panels; examples include Influenza A and SARS-CoV-2 diagnostics, where PCR allows rapid and accurate detection of infection [ 23 , 24 ]. NPS methods have been shown to be as accurate as PCR-based methods with regard to common viral RTI, with the crucial advantage of generating real-time data whilst also being more portable and requiring less laboratory infrastructure [ 25 , 26 ].…”

Section: Nanopore Sequencing In Rtimentioning

confidence: 99%

Nanopore-Based Metagenomic Sequencing in Respiratory Tract Infection: A Developing Diagnostic Platform

Chapman

Jones

D’Angelo

et al. 2023

Lung

View full text Add to dashboard Cite

Respiratory tract infection (RTI) remains a significant cause of morbidity and mortality across the globe. The optimal management of RTI relies upon timely pathogen identification via evaluation of respiratory samples, a process which utilises traditional culture-based methods to identify offending microorganisms. This process can be slow and often prolongs the use of broad-spectrum antimicrobial therapy, whilst also delaying the introduction of targeted therapy as a result. Nanopore sequencing (NPS) of respiratory samples has recently emerged as a potential diagnostic tool in RTI. NPS can identify pathogens and antimicrobial resistance profiles with greater speed and efficiency than traditional sputum culture-based methods. Increased speed to pathogen identification can improve antimicrobial stewardship by reducing the use of broad-spectrum antibiotic therapy, as well as improving overall clinical outcomes. This new technology is becoming more affordable and accessible, with some NPS platforms requiring minimal sample preparation and laboratory infrastructure. However, questions regarding clinical utility and how best to implement NPS technology within RTI diagnostic pathways remain unanswered. In this review, we introduce NPS as a technology and as a diagnostic tool in RTI in various settings, before discussing the advantages and limitations of NPS, and finally what the future might hold for NPS platforms in RTI diagnostics.

show abstract

Section: Nanopore Sequencing In Rtimentioning

confidence: 99%

Nanopore-Based Metagenomic Sequencing in Respiratory Tract Infection: A Developing Diagnostic Platform

Chapman

Jones

D’Angelo

et al. 2023

Lung

View full text Add to dashboard Cite

show abstract

“…Currently, influenza virus testing in most hospitals in the United Kingdom is based on clinical suspicion and the use of laboratory-based polymerase chain reaction (PCR) tests (Brendish et al, 2017; Elf et al, 2018). The turnaround times for laboratory-based PCR tests range from 24 to 48 hours.…”

Section: Introductionmentioning

confidence: 99%

Single centre experience: Managing seasonal influenza without a point-of-care test

Tee

2023

Journal of Infection Prevention

View full text Add to dashboard Cite

Background The use of point-of-care testing (POCT) for influenza is not routine in many hospitals in the United Kngdom and testing is currently by laboratory-based polymerase chain reaction (PCR) tests. This review aims to evaluate patients who were tested positive for influenza in the last winter season and project if future POCT usage at initial point of patient assessment could assist in better utilisation of healthcare resources. Methods Retrospective review in a district general hospital without POCT facility for influenza. Medical records of patients who tested positive for influenza during a 4-month period from 1 October 2019 to 31 January 2020 in the paediatric department were reviewed and analysed. Results Thirty patients had laboratory-confirmed cases of influenza of which 63% ( n = 19) were admitted to the ward. 56% of patients were not isolated at first instance on admission and 50% ( n = 9) of admitted patients did not require inpatient management which totalled up to 224 h length of stay in ward. Conclusions Routine POCT for influenza can potentially improve management of patients presenting with respiratory symptoms and allocation of healthcare resources. We recommend its use to be introduced in diagnostic pathways for acute respiratory illness in the paediatric population in the next winter season in all hospitals.

show abstract

“…Influenza virus testing in the UK is based on clinical suspicion and the use of laboratory-based PCR tests [10, 11]. In the UK, laboratory-based PCR turnaround times range at 24–48 h [10, 12].…”

Section: Introductionmentioning

confidence: 99%

Impact of a PCR point of care test for influenza A/B on an acute medical unit in a large UK teaching hospital: results of an observational, pre and post intervention study

Garvey

Wilkinson

Bradley

et al. 2019

Antimicrob Resist Infect Control

View full text Add to dashboard Cite

Background Influenza viruses is a leading cause of acute respiratory infection, placing a significant burden on healthcare. To reduce hospital transmission, patients clinically suspected of having influenza are isolated and offered empirical antiviral treatment. Here we report the use of a point of care test (POCT) for influenza viruses in an acute medical unit (AMU) at Queen Elizabeth Hospital Birmingham for patients presenting with influenza-like illness. Methods A PCR POCT was installed on AMU in Dec 17 – Mar 18 (period 2) and used to test any patient with influenza-like illness. We conducted an evaluation against influenza virus’s data collected between Dec 16–Mar 17 (period 1) where no POCT was used. Four outcomes were measured: length of stay, oseltamivir utilisation, time to isolation and in-hospital cases of influenza viruses. Results There were 51 confirmed influenza virus cases in period 1 vs 666 in period 2. During period 2, the length of stay of patients presenting with influenza-like illness (2.4 vs 7.9 days) and time to isolation from receipt of a positive result (0.09 vs 1.26 days) was significantly shorter. The time to initial receipt of antivirals for patients with influenza virus was significantly quicker in period 2 (0.59 vs 1.1 days) and the total number of influenza virus cases identified after 72 h of admission was significantly lower (9% vs 51%). Discussion Following introduction of the POCT, there was an increase in appropriately targeted oseltamivir prescribing, shorter time to isolation, proportionally less post-72-h influenza virus cases and a reduction in length of stay of patients presenting with influenza-like illness. Conclusions Routine use of POCTs for viruses should be introduced into diagnostic pathways for acute respiratory illness, especially at the front door of hospitals.

show abstract

Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens

Cited by 5 publications

References 23 publications

Nanopore-Based Metagenomic Sequencing in Respiratory Tract Infection: A Developing Diagnostic Platform

Nanopore-Based Metagenomic Sequencing in Respiratory Tract Infection: A Developing Diagnostic Platform

Single centre experience: Managing seasonal influenza without a point-of-care test

Impact of a PCR point of care test for influenza A/B on an acute medical unit in a large UK teaching hospital: results of an observational, pre and post intervention study

Contact Info

Product

Resources

About