Development and evaluation of a subunit vaccine for infectious bovine rhinotracheitis

Lupton, H W

doi:10.31274/rtd-180813-5437

Cited by 7 publications

(11 citation statements)

References 121 publications

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“…Despite continued use of BHV-1 vaccines, economic losses remain severe. The efficacy of both attenuated and killed BHV-1 vaccines has been reviewed (25,51,73). In general, present BHV-1 vaccines are able to protect against disease, tut none is effective in preventing infection with virulent challenge virus (51,84).…”

Section: Introductionmentioning

confidence: 99%

“…The efficacy of both attenuated and killed BHV-1 vaccines has been reviewed (25,51,73). In general, present BHV-1 vaccines are able to protect against disease, tut none is effective in preventing infection with virulent challenge virus (51,84). Disease control is hampered by the ability of BHV-1 (wild-type or vaccine origin) to establish latent infections.…”

Section: Introductionmentioning

confidence: 99%

“…vaccinates challenged with virulent BHV-1 appeared to be protected from infection as well as disease (51). Detergent treatment of the BHV-1 virion removed thirteen polypeptides which were thought to be associated with the virion envelope (3).…”

Section: Introductionmentioning

confidence: 99%

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Identification of bovine herpesvirus-1 polypeptides involved in serum neutralization

Lum

Reed

1986

Veterinary Microbiology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of bovine herpesvirus-1 polypeptides involved in serum neutralization

Lum

Reed

1986

Veterinary Microbiology

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“…As in other alphaherpesviruses, BHV-1 glycoproteins are the major structural components of the viral envelope and virus-infected cell membranes. The glycoproteins play important roles in viruscell interactions, including recognition and attachment of the virion and its penetration into susceptible cells (8,10,12), viral neutralization, and immune destruction of infected cells (7,13). Glycoprotein D (gD) of BHV-1, a homologue of herpes simplex virus gD, is one of the four essential major glycoproteins, together with gB, gC, and gH, which have been identified on the virus envelope and the plasma membrane of BHV-1 infected cells (22).…”

mentioning

confidence: 99%

Use of Epitope Mapping To Identify a PCR Template for Protein Amplification and Detection by Enzyme-Linked Immunosorbent Assay of Bovine Herpesvirus Type 1 Glycoprotein D

Joseph

Lyaku²,

Fredrickson³

et al. 2002

J Clin Microbiol

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Infection with bovine herpesvirus type 1 (BHV-1) occurs worldwide and causes serious economic losses due to the deaths of animals, abortions, decreased milk production, and loss of body weight. BHV-1 is frequently found in bovine semen and is transmitted through natural service and artificial insemination. The detection of BHV-1 in bovine semen is a long-standing problem in veterinary virology which is important in disease control schemes. In the present study, ordered deletions of the full-length BHV-1 glycoprotein open reading frame were used to identify an epitope recognized by a specific monoclonal antibody (MAb). A glycoprotein D fragment containing this epitope was then amplified using an in vitro protein amplification assay developed previously (J. Zhou, J. Lyaku, R. A. Fredrickson, and F. S. Kibenge, J. Virol. Methods 79:181-189, 1999), and the resulting peptide was detected by indirect enzyme-linked immunosorbent assay (ELISA) with the specific MAb. This method detected 0.0395 50% tissue culture infective dose of BHV-1 in raw bovine semen, which was 1,000-fold more sensitive than traditional PCR. We therefore conclude that this in vitro protein amplification assay combined with ELISA has superior sensitivity for direct virus detection in clinical samples.Bovine herpesvirus type 1 (BHV-1) is responsible for a variety of diseases in cattle, including respiratory and genital infections, conjunctivitis, abortion, and enteritis, causing great economic loss to the cattle industry worldwide (6). As in other alphaherpesviruses, BHV-1 glycoproteins are the major structural components of the viral envelope and virus-infected cell membranes. The glycoproteins play important roles in viruscell interactions, including recognition and attachment of the virion and its penetration into susceptible cells (8,10,12), viral neutralization, and immune destruction of infected cells (7,13). Glycoprotein D (gD) of BHV-1, a homologue of herpes simplex virus gD, is one of the four essential major glycoproteins, together with gB, gC, and gH, which have been identified on the virus envelope and the plasma membrane of BHV-1 infected cells (22). It stimulates a potent neutralizing antibody response in animals and induces significant protection against BHV-1-induced diseases. Moreover, BHV-1 gD is a very stable antigen whose epitopes do not change under selective pressure (18).It has also been reported that monoclonal antibodies (MAbs) against gD show the highest complement-independent virus-neutralizing activity and inhibit virus adsorption and penetration (4, 9, 23). MAbs against BHV-1 gD and their use in diagnostic tests, epitope mapping, and functional analysis have been reported by several investigators, including van Drunen et al. (22) BHV-1 is frequently found in bovine semen and can be widely transmitted through artificial insemination. The detection of BHV-1 in bovine semen is a long-standing problem in veterinary virology which is important in disease control schemes. None of the methods developed so far have been found who...

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“…BHV-1 contributes considerably to the causes of bovine respiratory diseases [ 10] and has been described as a significant component of the disease complex [23]. Although live attenuated vaccines stimulate cell-mediated immunity (CMI) as well as antibody response to BHV-1 [9], many investigators have reported shedding of BHV-1 from vaccinated animals, which may serve as a source of infection to susceptible cattle [17,39].…”

Section: Introductionmentioning

confidence: 99%

Anti-idiotypic antibodies to bovine herpesvirus-1 inhibit virus infection in cell cultures

Abdelmagid

Orten

Xue

et al. 1992

Archives of Virology

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A panel of murine monoclonal antibodies (MAbs) to bovine herpesvirus-1 (BHV-1) was prepared. Three of them were neutralizing MAbs and reacted against 130/75/50 kDa, 77 kDa, or 97 kDa glycoproteins (gp). A fourth non-neutralizing MAb recognized the 97 kDa gp. Competition radioimmunoassay demonstrated that each of the four MAbs reacted against a different virus epitope. Anti-idiotypic antibodies (anti-id) to the four MAbs were produced in rabbits and purified by sequential immunoaffinity chromatography. Each anti-id inhibited the binding of its respective MAb to BHV-1 in competitive ELISA and blocked BHV-1 neutralizing activity of the MAb. This inhibition suggested that the anti-ids were specific for the antigen binding site of the MAbs. Treatment of MDBK cells with anti-ids inhibited BHV-1 infection, which suggested that the anti-ids block a cellular component essential for virus infection. Absence of significant cross-reactivity among the anti-ids for heterologous MAbs indicated that they recognized unique determinants on the antigen binding site of the homologous MAb.

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Development and evaluation of a subunit vaccine for infectious bovine rhinotracheitis

Cited by 7 publications

References 121 publications

Identification of bovine herpesvirus-1 polypeptides involved in serum neutralization

Identification of bovine herpesvirus-1 polypeptides involved in serum neutralization

Use of Epitope Mapping To Identify a PCR Template for Protein Amplification and Detection by Enzyme-Linked Immunosorbent Assay of Bovine Herpesvirus Type 1 Glycoprotein D

Anti-idiotypic antibodies to bovine herpesvirus-1 inhibit virus infection in cell cultures

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