Development and Engineering of CSαβ Motif for Biomedical Application

Yang, Ying-Fang

doi:10.5772/19862

Cited by 2 publications

(2 citation statements)

References 97 publications

(139 reference statements)

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“…CSαβ‐containing defensins are commonly present in plants and insects and they have mainly shown antimicrobial activity against fungi and bacteria, respectively (de Oliveira Dias & Franco, 2015). In an amphipathic structure, the positively charged residues are usually located in the helix while the β‐sheet of the motif consists of hydrophobic amino acid residues (Yang, 2012). These amphipathic structures make possible the binding and disruption of bacterial cytoplasmic membranes of plectasin, which is a peptide antibiotic containing CSαβ motif from a saprophytic fungus Pseudoplectania nigrella (Schneider et al, 2010).…”

Section: Structural Classification Of Ampsmentioning

confidence: 99%

Antimicrobial peptides (AMPs): A promising class of antimicrobial compounds

Büyükkiraz

Kesmen

2022

Journal of Applied Microbiology

163

125

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Antimicrobial peptides (AMPs) are compounds, which have inhibitory activity against microorganisms. In the last decades, AMPs have become powerful alternative agents that have met the need for novel anti‐infectives to overcome increasing antibiotic resistance problems. Moreover, recent epidemics and pandemics are increasing the popularity of AMPs, due to the urgent necessity for effective antimicrobial agents in combating the new emergence of microbial diseases. AMPs inhibit a wide range of microorganisms through diverse and special mechanisms by targeting mainly cell membranes or specific intracellular components. In addition to extraction from natural sources, AMPs are produced in various hosts using recombinant methods. More recently, the synthetic analogues of AMPs, designed with some modifications, are predicted to overcome the limitations of stability, toxicity and activity associated with natural AMPs. AMPs have potential applications as antimicrobial agents in food, agriculture, environment, animal husbandry and pharmaceutical industries. In this review, we have provided an overview of the structure, classification and mechanism of action of AMPs, as well as discussed opportunities for their current and potential applications.

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Section: Structural Classification Of Ampsmentioning

confidence: 99%

Antimicrobial peptides (AMPs): A promising class of antimicrobial compounds

Büyükkiraz

Kesmen

2022

Journal of Applied Microbiology

163

125

View full text Add to dashboard Cite

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“…The supplemental head-to-tail cyclization further increases the stability of the secondary structure, without the need of additional conformational changes upon the interaction with cell membranes. Three or four disulphide bridges stabilize the α-helix/β-sheet mixed structures, that consist of positively charged residues arranged in the helix and of hydrophobic amino acids in the β-sheet motif ( Yang, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Revolutionizing orthopedic healthcare: a systematic review unveiling recombinant antimicrobial peptides

Pennone,

Rosini,

Mascheroni

et al. 2024

Front. Microbiol.

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The increasing demand for orthopedic surgeries, including joint replacements, is driven by an aging population and improved diagnosis of joint conditions. Orthopedic surgeries carry a risk of infection, especially in patients with comorbidities. The rise of antibiotic resistance exacerbates this issue, necessitating alternatives like in vitro bioengineered antimicrobial peptides (AMPs), offering broad-spectrum activity and multiple action mechanisms. This review aimed to assess the prevalence of antimicrobial potential and the yield after purification among recombinant AMP families. The antimicrobial potential was evaluated using the Minimum Inhibitory Concentration (MIC) values against the most common bacteria involved in clinical infections. This systematic review adhered to PRISMA guidelines, focusing on in vitro studies of recombinant AMPs. The search strategy was run on PubMed, Scopus and Embase up to 30th March 2023. The Population, Exposure and Outcome model was used to extract the data from studies and ToxRTool for the risk of bias analysis. This review included studies providing peptide production yield data and MIC values against pathogenic bacteria. Non-English texts, reviews, conference abstracts, books, studies focusing solely on chemical synthesis, those reporting incomplete data sets, using non-standard MIC assessment methods, or presenting MIC values as ranges rather than precise concentrations, were excluded. From 370 publications, 34 studies on AMPs were analyzed. These covered 46 AMPs across 18 families, with Defensins and Hepcidins being most common. Yields varied from 0.5 to 2,700 mg/L. AMPs were tested against 23 bacterial genera, with MIC values ranging from 0.125 to >1,152 μg/mL. Arenicins showed the highest antimicrobial activity, particularly against common orthopedic infection pathogens. However, AMP production yields varied and some AMPs demonstrated limited effectiveness against certain bacterial strains. This systematic review emphasizes the critical role of bioengineered AMPs to cope infections and antibiotic resistance. It meticulously evaluates recombinant AMPs, focusing on their antimicrobial efficacy and production yields. The review highlights that, despite the variability in AMP yields and effectiveness, Arenicins and Defensins are promising candidates for future research and clinical applications in treating antibiotic-resistant orthopedic infections. This study contributes significantly to the understanding of AMPs in healthcare, underscoring their potential in addressing the growing challenge of antibiotic resistance.Systematic review registration:https://osf.io/2uq4c/.

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Development and Engineering of CSαβ Motif for Biomedical Application

Cited by 2 publications

References 97 publications

Antimicrobial peptides (AMPs): A promising class of antimicrobial compounds

Antimicrobial peptides (AMPs): A promising class of antimicrobial compounds

Revolutionizing orthopedic healthcare: a systematic review unveiling recombinant antimicrobial peptides

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