Development and efficacy of a novel streptomycin-resistant Flavobacterium johnsoniae vaccine in grass carp (Ctenopharyngodon idella)

Li, Ningqiu; Lin, Qiang; Fu, Xiaozhe; Guo, Huiling; Liu, Lihui; Shu-qin, WU

doi:10.1016/j.aquaculture.2015.05.047

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…One approach for generating avirulent strains is serial passage of pathogenic strains on growth media. Li et al (2015) showed that a serially passaged pathogenic strain of S. agalactiae lost its virulence. Immersion vaccination with live S. agalactiae attenuated vaccine (YM001) gave an RPS of 67.22%.…”

Section: Streptococcosis Lactococcosis Enterococcosismentioning

confidence: 99%

“…Grass carp (Ctenopharyngodon idella) with a mean weight of 10.1 g were bathed in 1 × 10 7 CFU mL −1 of the attenuated vaccine for 30 min. At 28 and 240-days post-vaccination (dpv) the fish were challenged with virulent F. johnsoniae M168 by immersion in 1 × 10 7 CFU mL −1 for 30 min [93]. The results showed that fish vaccinated with the attenuated strain had a RPS of 73.1 and 60 when challenged 28 days post-vaccination (dvp) and 240 dpv, respectively, meaning that protection lasted for up to 8 months.…”

Section: Flavobacteriosismentioning

confidence: 99%

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Review on Immersion Vaccines for Fish: An Update 2019

Bøgwald

Dalmo

2019

Microorganisms

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Immersion vaccines are used for a variety of aquacultured fish to protect against infectious diseases caused by bacteria and viruses. During immersion vaccination the antigens are taken up by the skin, gills or gut and processed by the immune system, where the resulting response may lead to protection. The lack of classical secondary responses following repeated immersion vaccination may partly be explained by the limited uptake of antigens by immersion compared to injection. Administration of vaccines depends on the size of the fish. In most cases, immersion vaccination is inferior to injection vaccination with regard to achieved protection. However, injection is problematic in small fish, and fry as small as 0.5 gram may be immersion vaccinated when they are considered adaptively immunocompetent. Inactivated vaccines are, in many cases, weakly immunogenic, resulting in low protection after immersion vaccination. Therefore, during recent years, several studies have focused on different ways to augment the efficacy of these vaccines. Examples are booster vaccination, administration of immunostimulants/adjuvants, pretreatment with low frequency ultrasound, use of live attenuated and DNA vaccines, preincubation in hyperosmotic solutions, percutaneous application of a multiple puncture instrument and application of more suitable inactivation chemicals. Electrostatic coating with positively charged chitosan to obtain mucoadhesive vaccines and a more efficient delivery of inactivated vaccines has also been successful.

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Section: Streptococcosis Lactococcosis Enterococcosismentioning

confidence: 99%

Section: Flavobacteriosismentioning

confidence: 99%

Review on Immersion Vaccines for Fish: An Update 2019

Bøgwald

Dalmo

2019

Microorganisms

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“…As a promising prophylaxis, vaccination is commonly considered a major improvement to induce immune protective effects against fish disease (Li et al, 2015; Wang et al, 2016; Zhang, Li, et al, 2018; Zhu et al, 2017). Our previous study showed that the MCP gene of LBUSV could be used as an antigen for DNA vaccine constructs, with the highest protection rate of 61.11% in largemouth bass (Jia et al, 2020a).…”

Section: Discussionmentioning

confidence: 99%

Antigenic epitope screening and functional modification of mannose enhance the efficacy of largemouth bass virus subunit vaccines

Jia

Xia

Jiang

et al. 2022

Journal of Fish Diseases

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Major capsid protein (MCP) can be used as a subunit vaccine against largemouth bass virus (LMBV). However, subunit vaccines usually have low immunogenicity. Here, to identify the major immunogenicity determinant region of the MCP gene, we truncated the MCP of the LMBV gene into four parts (MCP‐1, MCP‐2, MCP‐3 and MCP‐4). Enzyme‐linked immunosorbent assay (ELISA) was used to identify the antigenicity of these four truncated MCP proteins. Then, the highly antigenic truncated protein was modified with mannose and connected with functionalized single‐walled carbon nanotubes (SWCNTs) as carriers. Largemouth basses were immunized by bath immersion, challenged with LMBV on the 28th day after immunization and evaluated for related immune indicators. The results indicated that the MCP‐2 protein could induce a higher antibody titre than the other truncated MCP proteins. We found that the levels of immune‐related genes (TNF‐α, CD40, IgM, IFNγ and IL‐10) in the spleen and kidney were significantly increased in the MCP‐2 and MCP‐2‐Man groups. ELISA results showed that the antibody content in the serum increased significantly in the MCP‐2 group 7 days post‐vaccination and increased with days in all the vaccinated groups, with the highest observed on the 21st day. Notably, the MCP‐2‐Man vaccine (10 mg L−1) showed durability of immunoprotection efficacy that could protect largemouth basses from LMBV challenge, and the immune protection rate reached 78.94%. These results suggest that MCP‐2 might be the major immunogenicity determinant region of LMBV and that the mannose‐modified MCP‐2 vaccine can induce stronger adaptive immune responses.

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“…As reported, Massilia is often isolated from some extreme environments and can produce polyhydroxyalkanoate ; while Flavobacterium spp. often isolated from water or plants shows resistance to multiple antibiotics such as rifampicin and streptomycin . In addition, Cellvibrio , Methylotenera , Candidatus_Amoebophilus , Emticicia , Leadbetterella , Arcicella , and Aureispira were only detected as the metabolically active genera in Hygroamblystegium noterophilum .…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of bacteria associated with different mosses by 16S rRNA and 16S rDNA sequencing

Tian

2016

J. Basic Microbiol.

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To understand the differences of the bacteria associated with different mosses, a phylogenetic study of bacterial communities in three mosses was carried out based on 16S rDNA and 16S rRNA sequencing. The mosses used were Hygroamblystegium noterophilum, Entodon compressus and Grimmia montana, representing hygrophyte, shady plant and xerophyte, respectively. In total, the operational taxonomic units (OTUs), richness and diversity were different regardless of the moss species and the library level. All the examined 1183 clones were assigned to 248 OTUs, 56 genera were assigned in rDNA libraries and 23 genera were determined at the rRNA level. Proteobacteria and Bacteroidetes were considered as the most dominant phyla in all the libraries, whereas abundant Actinobacteria and Acidobacteria were detected in the rDNA library of Entodon compressus and approximately 24.7% clones were assigned to Candidate division TM7 in Grimmia montana at rRNA level. The heatmap showed the bacterial profiles derived from rRNA and rDNA were partly overlapping. However, the principle component analysis of all the profiles derived from rDNA showed sharper differences between the different mosses than that of rRNA-based profiles. This suggests that the metabolically active bacterial compositions in different mosses were more phylogenetically similar and the differences of the bacteria associated with different mosses were mainly detected at the rDNA level. Obtained results clearly demonstrate that combination of 16S rDNA and 16S rRNA sequencing is preferred approach to have a good understanding on the constitution of the microbial communities in mosses.

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Development and efficacy of a novel streptomycin-resistant Flavobacterium johnsoniae vaccine in grass carp (Ctenopharyngodon idella)

Cited by 11 publications

References 22 publications

Review on Immersion Vaccines for Fish: An Update 2019

Review on Immersion Vaccines for Fish: An Update 2019

Antigenic epitope screening and functional modification of mannose enhance the efficacy of largemouth bass virus subunit vaccines

Comparative analysis of bacteria associated with different mosses by 16S rRNA and 16S rDNA sequencing

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