Development and comparison of UPLC-ESI-MS and RP-HPLC-VWD methods for determining microcystin-LR

Jin, Peng; Yang, Kai; Bai, Ruining; Chen, Mei; Yang, Shilin; Fu, Kebo; He, Jieli

doi:10.1039/d1ra03521e

Cited by 12 publications

(8 citation statements)

References 43 publications

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“…Considering that the outliers may affect the accuracy and precision of the results, the Grubbs test was used to determine whether outliers should be discarded. 61 The G value was calculated by formula (1) . where x q is the questionable value and x̄ is the average value.…”

Section: Resultsmentioning

confidence: 99%

Facile synthesis of carbon nitride quantum dots as a highly selective and sensitive fluorescent sensor for the tetracycline detection

Bai

Jin

et al. 2021

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

Facile synthesis of carbon nitride quantum dots as a highly selective and sensitive fluorescent sensor for the tetracycline detection

Bai

Jin

et al. 2021

RSC Adv.

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“…This result can be attributed to the structure of MC-LR that has Arg residues. It also demonstrates that MC-LR is a cyclic heptapeptide compound, which is liable to form multi-charged ions [M + nH] n+ in the electrospray ionization source (Jin et al, 2021).…”

Section: Pegylated Go and Its Conjugation With Aptamersmentioning

confidence: 94%

Aptamer-conjugated graphene oxide for enrichment of microcystin-LR prior to matrix-assisted laser desorption ionization mass spectrometry

Feng

Song

et al. 2022

Preprint

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Microcystin-LR (MC-LR) is a cyclic heptapeptide with hepatotoxic and neurotoxic activities in mammals, which is the most widespread variant of MCs produced by cyanobacterial in eutrophic water. Here, an effective adsorbent using aptamer modified graphene oxide (GO) was designed to detect MC-LR at low concentrations. GO was functionalized with an MC-LR aptamer (Apt-3) by surface modification using polyethylene glycol (PEG). Then, the novel aptamer-conjugated GO (Apt-3/PEG/GO) was used to enrich and separate MC-LR from pond water or freshwater snail samples following by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) analysis. Results indicated that the Apt-3/PEG/GO nanocomposites were highly specific to MC-LR, and the detection limit of MALDI-MS was 0.50 ng/mL in spiked, environmental water samples. Moreover, the MC-LR can be released from nanocomposites by heating, thus the reuse of Apt-3/PEG/GO is realized. Real samples analysis indicated that the Apt-3/PEG/GO nanocomposites coupled with MALDI-MS was efficient in detection trace amount of MC-LR in snail samples. With the merits of low cost, reusable, easy to synthesis and to flexible assembly, the Apt-3/PEG/GO nanocomposites is a novel adsorbent with extensive applications, and its combination with MALDI-MS is expected to assess other analytes by anchoring suitable aptamers for different targets.

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“…Until now, numerous analytical methods have been reported for MC-LR quantification. Although sophisticated instruments, including high performance liquid chromatography (HPLC) , and liquid chromatography-mass spectrometry (LC-MS) − with low detection limits are powerful in MC-LR quantification, the requirements of bulky and expensive apparatus complicate sample pretreatment. Meanwhile, well-trained technicians hinder its extensive and on-site applications.…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater

Kang

et al. 2022

Environ. Sci. Technol.

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On-site monitoring of trace organic pollutants with facile methods is critical to environmental pollutant prevention and control. Herein, we proposed a CRISPR-Cas12a-based aptasensor platform (named as MC-LR-Casor) for on-site and sensitive detection of microcystin-LR (MC-LR). After hybridization with blocker DNA, the MC-LR aptamers were conjugated to magnetic beads (MBs) to get the MB aptasensor. In the presence of MC-LR, their interactions with aptamers were triggered and the specific binding caused the release of blocker DNA. Using the programmability of the CRISPR-Cas system, the released blocker DNA was designed to activate a Cas12a-crRNA complex. Single strand DNA reporters were rapidly cleaved by the complex. Signal readout could be achieved by fluorometer or lateral flow strips, which were positively correlated to MC-LR concentration. Benefiting from the CRISPR-Cas12a amplification system, the proposed sensing platform exhibited high sensitivity and reached the limit of detection of ∼3 × 10–6 μg/L (fluorescence method) or 1 × 10–3 μg/L (lateral flow assay). In addition, the MC-LR-Casor showed excellent selectivity and good recovery rates, demonstrating their good applicability for real water sample analysis. During the whole assay, only two steps of incubation at a constant temperature were required and the results could be visualized when employing flow strips. Therefore, the proposed assay offered a simple and convenient alternative for in situ MC-LR monitoring, which may hold great promise for future environmental surveillance.

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Development and comparison of UPLC-ESI-MS and RP-HPLC-VWD methods for determining microcystin-LR

Abstract: UPLC-MS and HPLC-VWD methods were used together for MC-LR determination in a wide concentration range. UPLC-MS can be applied in trace MC-LR determination, whereas HPLC-VWD is more suitable for high concentration range detection.

Cited by 12 publications

References 43 publications

Facile synthesis of carbon nitride quantum dots as a highly selective and sensitive fluorescent sensor for the tetracycline detection

Facile synthesis of carbon nitride quantum dots as a highly selective and sensitive fluorescent sensor for the tetracycline detection

Aptamer-conjugated graphene oxide for enrichment of microcystin-LR prior to matrix-assisted laser desorption ionization mass spectrometry

CRISPR-Cas12a-Based Aptasensor for On-Site and Highly Sensitive Detection of Microcystin-LR in Freshwater

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