Development and comparison of two multi-residue methods for the analysis of select pesticides in honey bees, pollen, and wax by gas chromatography–quadrupole mass spectrometry

Li, Yuanbo; Kelley, Rebecca A.; Anderson, Troy D.; Lydy, Michael J.

doi:10.1016/j.talanta.2015.03.031

Cited by 54 publications

(27 citation statements)

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“…Honeybees come into contact with pesticides because veterinary practices expose them to pesticides such as acaricides required to control bee parasites like Varroa destructor and Varroa jacobsonie (Li et al, 2015) and fungicides to control Ascosphera apis, as well as other chemical agents such as antibiotics, and sulphonamides used to control bacterial diseases like European foulbrood (Streptococcus pluton) or American foulbrood (Bacillus larvae).…”

Section: Introductionmentioning

confidence: 99%

Mixture-risk-assessment of pesticide residues in retail polyfloral honey

2016

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The presence of even tiny quantities of pesticide residues in honey, a traditional healthy product, is a matter of concern for producers, packers and consumers. This paper aims to quantify pesticides in retail brands of polyfloral honey, and to calculate the mixture risk assessment of honey for consumers according to the results obtained from the analyzed samples. A LC-MS/MS multi-residue method was developed and validated for 13 compounds: 11 pesticides (chlorfenvinphos, coumaphos, tau-fluvalinate, amitraz, which are very common in veterinary treatments, and imidacloprid, acetamiprid, simazine, cyproconazole, tebuconazole, chlorpiryphos-methyl, chlorpiryphos, widely used in agricultural practices), and 2 metabolites of amitraz (2,. Results showed that the samples contained pesticide residues at different concentrations; however, the MRL in honey for each of the 11 pesticides was never exceeded. The most common were amitraz (from 1 to 50 µg/kg) present in 100% of the samples, and coumaphos (up to 14 µg/kg) in 63%. The hazard index (HI) for adults was less than 0.002 in all cases, a long way from 1, the value established as the limit of acceptability.Therefore, commercial honey does not represent any significant risk to health. However, considering that residue levels should be present "as low as reasonably achievable" it is deemed necessary to make an effort to reduce their presence by appropriate agricultural and, above all, beekeeping practices due to acaridae treatments.Keywords: Honey; pesticides; Mixture-risk-assessment; MRL; hazard index 2 IntroductionHoney is a highly valued natural product due to its nutritional properties and appreciated therapeutic applications. However, recently, food alerts caused by the detection of antibiotics, pesticides or heavy metals in honey have jeopardized its healthy image (JuanBorrás et al., 2015). Pesticide residues in honey come from environmental pollution and veterinary practices (Totti et al., 2006;Calatayud-Vernich et al., 2016 , 2016). However, this is the first attempt to apply this approach to retail honey.Therefore, the objective of this paper was to quantify the presence of different pesticides (usually found in the routine control analyses of honey samples) in polyfloral labeled brands, and to estimate the mixture risk assessment of the honey for consumers (due to exposure to pesticides) based on the results obtained from the analyzed samples. As a first step, the analytical procedure was validated in order to guarantee the quality of the results obtained. Material and Methods Honey samplesThis study was carried out with supermarket own-brands and well-known brand honey, which represent almost all of the retail sales in the Spanish market. A total of 22 honey samples, labelled as polyfloral were purchased across Spain from different retail outlets.Samples were purchased with as recent a packing date as possible, based on the label information, and were kept frozen until analysis to minimize losses of target compounds.A mixture of 5 polyfloral honeys w...

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Section: Introductionmentioning

confidence: 99%

Mixture-risk-assessment of pesticide residues in retail polyfloral honey

2016

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“…High molecular weight compounds that are lipid-like and long-chain fatty acids can reduce signal and/or cause column damage; therefore, additional cleanup is necessary to reduce the matrix constituents before injection [50]. In the current study, the analytical curves in acetonitrile and blank matrix extract were constructed to evaluate and compensate for the matrix effect (ME) and to assess the linearity according to the procedures for method validation suggested by SANCO [48]. So the matrix effects (ME) were evaluated by comparing the slope of the calibration curve in matrix with the slope of the calibration curve in solvent according to the following equation [51] slope of calibration curve in matrix ME(%) 1 100 slope of calibration curve in solvent…”

Section: Matrix Effectsmentioning

confidence: 99%

“…The proposed procedure has been evaluated according to the Method Validation and Quality Control Procedures for Pesticides Residues Analysis in Food and Feed [48] in terms of repeatability, linearity and recovery. The limits of detection (LOD) and quantification (LOQ) were estimated based on the preliminary calibration curve in acetonitrile, in the concentration range of 2-100 ng/g.…”

Section: Analytical Protocol Performancementioning

confidence: 99%

“…The samples of dead honeybees and honey was collected from the most contaminated areas of Pomerania in Poland (Tczew, Gdansk, Kartuzy) from suspected pesticide poisoning [46,47]. The methodology was optimized and next it was validated according to the regulation "Method Validation and Quality Control Procedures for Pesticide Residues Analysis in Food and Feed" [48]. This methodology allows for the monitoring of pesticides belonging to various classes for use of bee organisms and their products as the indicators of environmental contamination.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Gas Chromatography - Tandem Mass Spectrometry Procedure for Determination of Pesticide Residues in Honey and Honeybee Samples

Â¹,

M²

2015

J Chromatograph Separat Techniq

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A new analytical procedure of determination of multiresidue of pesticides based on application of the modified QuEChERS approach at the step of sample preparation and technique GC-MS/MS has been developed and applied for studies the real honey and honeybee samples. Samples were collected from northern Poland (Pomerania). The proposed procedure enables for the determination of 34 and 30 pesticide residues respectively in honey and honeybee samples. The recovery of analytes was between 70 and 120% with relative standard deviation ≤ 20%. The limits of quantification were in the range of 2.3-27 ng/g and 2.2-29 ng/g respectively for honey and honeybee samples. The proposed procedure was proven to be a powerful, highly sensitive, and environmentally friendly analytical tool that requires minimal sample preparation.

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“…Em 2015, foi desenvolvido o método analítico com o intuito de ajudar a explicar a perda de colônias de abelhas em todo o mundo, para quantificar 11 resíduos de agrotóxicos em amostras de abelhas Apis mellífera, pólen e cera, as quais foram coletadas no estado de Virginia, nos EUA. Dos 11 analitos avaliados, 10 foram detectados em pelo menos uma amostra (LI et al, 2015).…”

Section: Abelhasunclassified

Desenvolvimento e validação de método multirresidual para determinação de agrotóxicos em mel por cromatografia líquida de ultra eficiência acoplada a espectrometria de massas em série

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Development and comparison of two multi-residue methods for the analysis of select pesticides in honey bees, pollen, and wax by gas chromatography–quadrupole mass spectrometry

Cited by 54 publications

References 38 publications

Mixture-risk-assessment of pesticide residues in retail polyfloral honey

Mixture-risk-assessment of pesticide residues in retail polyfloral honey

Development of a Gas Chromatography - Tandem Mass Spectrometry Procedure for Determination of Pesticide Residues in Honey and Honeybee Samples

Desenvolvimento e validação de método multirresidual para determinação de agrotóxicos em mel por cromatografia líquida de ultra eficiência acoplada a espectrometria de massas em série

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