“Development and comparison of reverse transcription-loop-mediated isothermal amplification assay (RT-LAMP), RT-PCR and real time PCR for detection of Potato spindle tuber viroid in potato”

Verma, G. S.; Raigond, Baswaraj; Pathania, Shruti; Kochhar, Tarvinder; Naga, Kailash Chandra

doi:10.1007/s10658-020-02129-z

Cited by 13 publications

(8 citation statements)

References 20 publications

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“…This result is consistent with the results of previous studies (Lan et al, 2013; Sunani et al, 2019). RT‐LAMP was also previously found to be 10 times more sensitive than conventional RT‐PCR (Anandakumar et al, 2018; Verma et al, 2020; Wang et al, 2016). RT‐LAMP has a high amplification efficiency, partly because of its isothermal properties (Notomi et al, 2000).…”

Section: Discussionmentioning

confidence: 94%

“…When a large number of samples are detected, these molecular biological detection methods are complicated, time‐consuming and expensive, but loop‐mediated isothermal amplification (LAMP) technology embodies obvious advantages such as simple operation, rapid operation and low cost. What's more, in terms of the sensitivity of the detection technology, RT‐LAMP is 10 times more efficient than RT‐PCR (Anandakumar et al, 2018; Verma et al, 2020; Wang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Rapid detection of Broad bean wilt virus 2 and Turnip mosaic virus in Pseudostellaria heterophylla by reverse transcription loop‐mediated isothermal amplification assay

Liang

Chen

Liu

et al. 2022

Journal of Phytopathology

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Pseudostellaria heterophylla (P. heterophylla) is a widespread Chinese herb that is widely used as medicine, especially for the treatment of lung disease and nourishing the spleen. Broad bean wilt virus 2 (BBWV2) and Turnip mosaic virus (TuMV) threaten the quality and yield of P. heterophylla. To improve the detection efficiency of P. heterophylla viral disease pathogens, a rapid, simple, intuitive, accurate, sensitive and economical reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) method was established. Primers were designed based on the conserved sequences of BBWV2 and TuMV coat protein nucleotide sequences. The specificity of the primers and sensitivity of the LAMP method to the cDNA template were analysed by real‐time fluorescence‐based quantitative PCR, SYBR Green I staining, and agarose gel electrophoresis. No cross‐reactions occurred with other viruses tested in P. heterophylla. The detection limit of RT‐LAMP was 10 pg/μl and was found to be 10 times more sensitive on comparing with RT‐LAMP and reverse transcription‐PCR (RT‐PCR). Furthermore, the tube‐closed dye method facilitated the inspection of the LAMP reaction while avoiding cross‐contamination. This detection assay could serve as an important tool for field samples diagnoses and forecasting P. heterophylla viral disease intensities.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Rapid detection of Broad bean wilt virus 2 and Turnip mosaic virus in Pseudostellaria heterophylla by reverse transcription loop‐mediated isothermal amplification assay

Liang

Chen

Liu

et al. 2022

Journal of Phytopathology

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“…The detection of potato viruses typically requires field samples to be sent to a well-equipped laboratory for nucleic acid extraction and purification followed by RT-PCR and melt curve analysis. More recently, there has been an effort to replace the complex RT-PCR step with simpler isothermal amplification methods [ 38 , 39 , 40 , 41 , 42 ]. Therefore, we set out to develop a rapid, simple, and inexpensive approach for the detection of potato viruses that (a) minimizes sample processing; (b) provides reliable samples for the rapid and robust on-site diagnosis of infections by isothermal amplification; and (c) allows on-site verification by LFD analysis.…”

Section: Discussionmentioning

confidence: 99%

Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification

Edgü

Freund

Hartje³

et al. 2020

IJMS

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Potato is an important staple food crop in both developed and developing countries. However, potato plants are susceptible to several economically important viruses that reduce yields by up to 50% and affect tuber quality. One of the major threats is corky ringspot, which is a tuber necrosis caused by tobacco rattle virus (TRV). The appearance of corky ringspot symptoms on tubers prior to commercialization results in ≈ 45% of the tubers being downgraded in quality and value, while ≈ 55% are declared unsaleable. To improve current disease management practices, we have developed simple diagnostic methods for the reliable detection of TRV without RNA purification, involving minimalized sample handling (mini), subsequent improved colorimetric loop-mediated isothermal amplification (LAMP), and final verification by lateral-flow dipstick (LFD) analysis. Having optimized the mini-LAMP-LFD approach for the sensitive and specific detection of TRV, we confirmed the reliability and robustness of this approach by the simultaneous detection of TRV and other harmful viruses in duplex LAMP reactions. Therefore, our new approach offers breeders, producers, and farmers an inexpensive and efficient new platform for disease management in potato breeding and cultivation.

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“…Compared with qRT-PCR, RT-LAMP has obvious advantages. For example, certain mandatory equipment for polymerase chain reaction is no longer needed, and it has the advantages of easy operation, simple use, rapidness, high sensitivity, and specificity [ 75 ]. It enables rapid screening, greatly shortening the detection time.…”

Section: Nucleic Acid-based Detection Methodsmentioning

confidence: 99%

Recent advances in detection technologies for COVID-19

Han

Cong

Shen

et al. 2021

Talanta

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Corona Virus Disease 2019 (COVID-19) is a highly infectious respiratory illness that was caused by the SARS-CoV-2. It spread around the world in just a few months and became a worldwide pandemic. Quick and accurate diagnosis of infected patients is very important for controlling transmission. In addition to the commonly used Real-time reverse-transcription polymerase chain reaction (RT-PCR) detection techniques, other diagnostic techniques are also emerging endlessly. This article reviews the current diagnostic methods for COVID-19 and discusses their advantages and disadvantages. It provides an important reference for the diagnosis of COVID-19.

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“Development and comparison of reverse transcription-loop-mediated isothermal amplification assay (RT-LAMP), RT-PCR and real time PCR for detection of Potato spindle tuber viroid in potato”

Cited by 13 publications

References 20 publications

Rapid detection of Broad bean wilt virus 2 and Turnip mosaic virus in Pseudostellaria heterophylla by reverse transcription loop‐mediated isothermal amplification assay

Rapid detection of Broad bean wilt virus 2 and Turnip mosaic virus in Pseudostellaria heterophylla by reverse transcription loop‐mediated isothermal amplification assay

Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification

Recent advances in detection technologies for COVID-19

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