Development and Comparative Evaluation of a Competitive ELISA with Rose Bengal Test and a Commercial Indirect ELISA for Serological Diagnosis of Brucellosis

Tadepalli, Mythili; Lingala, Rajendra; Trangadia, B. J.; Thiagarajan, D.; Srinivasan, V.A.

doi:10.1007/s12088-011-0151-0

Cited by 12 publications

(5 citation statements)

References 7 publications

(8 reference statements)

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“…To further characterize the antibody reactions against various VP1 in Enterovirus genus, a competitive inhibition ELISA was conducted as described 37 38 39 . Briefly, the 96-well microtiter plates were coated with 1.0 μg of EV71 VP1 protein in 100 mM carbonate buffer (pH 9.6) overnight at 4 °C and then blocked for 2 h at 37 °C with 200 μl of 15% skimmed milk prepared in PBS-Tween 20.…”

Section: Methodsmentioning

confidence: 99%

Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals

Gao

Ding

Zhou

et al. 2016

Sci Rep

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The overall serological prevalence of EV infections based on ELISA remains unknown. In the present study, the antibody responses against VP1 of the EV-A species (enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A5 (CA5) and Coxsackievirus A6 (CA6)), of the EV-B species (Coxsackievirus B3 (CB3)), and of the EV-C species (Poliovirus 1 (PV1)) were detected and analyzed by a NEIBM (novel evolved immunoglobulin-binding molecule)-based ELISA in Shanghai blood donors. The serological prevalence of anti-CB3 VP1 antibodies was demonstrated to show the highest level, with anti-PV1 VP1 antibodies at the second highest level, and anti-CA5, CA6, CA16 and EV71 VP1 antibodies at a comparatively low level. All reactions were significantly correlated at different levels, which were approximately proportional to their sequence similarities. Antibody responses against EV71 VP1 showed obvious differences with responses against other EV-A viruses. Obvious differences in antibody responses between August 2013 and May 2014 were revealed. These findings are the first to describe the detailed information of the serological prevalence of human antibody responses against the VP1 of EV-A, B and C viruses, and could be helpful for understanding of the ubiquity of EV infections and for identifying an effective approach for seroepidemiological surveillance based on ELISA.

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Section: Methodsmentioning

confidence: 99%

Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals

Gao

Ding

Zhou

et al. 2016

Sci Rep

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“…To estimate the contribution of full length VP1 and various truncated VP1 proteins to the anti-EV71 VP1 reaction, the competitive inhibition ELISA was conducted as described 55 56 57 . Briefly, the 96-well microtiter plates was coated with 1.0 μg EV71 VP1 protein in 50 mM carbonate buffer (pH 9.6) overnight at 4 °C and then were blocked for 2 h at 37 °C with 200 μl of 15% skimmed milk prepared in PBS-Tween 20.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA

Ding

Chen

Bian

et al. 2015

Sci Rep

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Human enterovirus 71 (EV71) has become the major pathogen of hand, foot, and mouth disease (HFMD) worldwide, while the anti-EV71 antibody responses other than neutralizing epitopes have not been characterized. In this study, EV71 capsid proteins VP1, VP3, VP0 and various VP1 antigens were constructed to analyze anti-EV71 response in severe HFMD cases, non-HFMD outpatient children and normal adults using a novel evolved immunoglobulin-binding molecule (NEIBM)-based ELISA. The high prevalence of antibody responses against all three capsid proteins was demonstrated, and anti-EV71 VP1 showed the main antibody response. Anti-EV71 VP1 antibody response was found to predominantly target to epitopes based on the common enterovirus cross-reactive sequence. Moreover, inhibition pattern against anti-EV71 VP1 reactions in three groups was obviously different. Taken together, these results firstly characterized the anti-EV71 antibody responses which are predominantly against VP1 epitopes based on common enterovirus cross-reactive sequence. This finding could be helpful for the better understanding of anti-EV71 humoral immunity and useful for seroepidemiological surveillance.

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“…Serological and molecular epidemiological studies can help in elucidating the genetic diversity and evolution of these pathogens [99]. It has also been observed that the studies included in the present meta-analysis commonly used techniques like biochemical tests and PCR [44,56,97] followed by ELISA [34,37,64] and lateral flow assays [33] for the detection of bacterial pathogens. It is very much desired that longitudinal studies are needed to monitor the changes in prevalence over time and to assess the effectiveness of control measures.…”

Section: Discussionmentioning

confidence: 99%

Meta-Analysis of the Prevalence of Porcine Zoonotic Bacterial Pathogens in India: A 13-Year (2010–2023) Study

Rajkhowa,

Sonowal,

Borthakur

et al. 2023

Pathogens

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The presence of bacterial pathogens such as Brucella spp., Clostridium spp., E. coli, Listeria monocytogenes, Salmonella spp., Staphylococcus spp., and Streptococcus suis not only hampers pig production but also carries significant zoonotic implications. The present study aims to conduct a comprehensive meta-analysis spanning over 13 years (2010–2023) to ascertain the prevalence of these zoonotic bacterial pathogens in Indian pig populations. The study seeks to synthesize data from diverse geographic regions within India and underscores the relevance of the One Health framework. A systematic search of electronic databases was meticulously performed. Inclusion criteria encompassed studies detailing zoonotic bacterial pathogen prevalence in pigs within India during the specified timeframe. Pertinent information including authors, publication year, geographical location, sampling techniques, sample sizes, and pathogen-positive case counts were meticulously extracted. The meta-analysis of zoonotic bacterial pathogens in Indian pig populations (2010–2023) unveiled varying prevalence rates: 9% Brucella spp., 22% Clostridium spp., 19% E. coli, 12% Listeria monocytogenes, 10% Salmonella spp. and Streptococcus suis, and 24% Staphylococcus spp. The application of random effects further revealed additional variability: 6% Brucella spp., 23% Clostridium spp., 24% E. coli, 14% Listeria monocytogenes, 10% Salmonella spp. and Streptococcus suis, and 35% Staphylococcus spp. Notably, the observed heterogeneity (I2) varied significantly from 87% to 99%. The meta-analysis findings underscore the pervasive nature of these diseases throughout India’s pig populations, accentuating the substantial impact of these pathogens on pig health and the potential for zoonotic transmission. The present study reinforces the importance of the adoption of a comprehensive One Health approach that acknowledges the intricate interplay between animal, human and environmental health.

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Development and Comparative Evaluation of a Competitive ELISA with Rose Bengal Test and a Commercial Indirect ELISA for Serological Diagnosis of Brucellosis

Cited by 12 publications

References 7 publications

Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals

Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals

Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA

Meta-Analysis of the Prevalence of Porcine Zoonotic Bacterial Pathogens in India: A 13-Year (2010–2023) Study

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