Development and characterization of twelve microsatellite markers for Porphyra linearis Greville

Abstract

The genus Porphyra (and its sister genus Pyropia) contains important red algal species that are cultivated and/or harvested for human consumption, sustaining a billion-dollar aquaculture industry. A vast amount of research has been focused on species of this genus, including studies on genetics and genomics among other areas. Twelve novel microsatellite markers were developed here for Porphyra linearis. Markers were characterized using 32 individuals collected from four natural populations of P. linearis with total heterozygosity varying from 0.098 to 0.916. The number of alleles per locus ranged from 2 to 18. All markers showed cross amplification with Porphyra umbilicalis and/or Porphyra dioica. These polymorphic microsatellite markers are useful for investigating population genetic diversity and differentiation in P. linearis and may become useful for other genetic research on the reproductive biology of this important species.

Polyploid lineages in the genus Porphyra

“…Genomic DNA was isolated using the LiCl extraction protocol described by 30 as modified by 31 . Genotyping using 11 selected microsatellite markers 32 , 33 generated 121 multilocus genotypes (see Table S1 for sources, loci and amplification details). Polymerase chain reactions (PCR) for all markers were performed separately in a 20 μl reaction volume containing 5–50 ng genomic DNA.…”

“…Polymerase chain reactions (PCR) for all markers were performed separately in a 20 μl reaction volume containing 5–50 ng genomic DNA. Amplifications using an Applied Biosystems thermal cycler (GeneAmp 2720) were conducted by the PCR programs and conditions described in 32 , 33 . Amplified fragments were separated electrophoretically using an ABI PRISM 3130xl (Applied Biosystems) automated capillary sequencer at CCMAR, Portugal, and sized with GeneScan-350ROX size standard.…”

Isolation and characterization of microsatellite markers for the red alga Porphyra umbilicalis

“…Genomic DNA was isolated using a nuclei isolation protocol (Varela-Álvarez et al, 2006) followed by a LiCl extraction protocol described by Hong et al (1992) as modified by van Oppen et al (1995). Four microsatellite enriched libraries were constructed following Varela-Álvarez et al (2017), based on Glenn and Schable (2005) and Billote et al (1999). We also used nucleotide repeats that were complementary to those in other Porphyra spp.…”

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia&nbsp;yezoensis</em> Sookwawon&nbsp;104 with <em>in&nbsp;vitro</em> antiproliferative activity

“…Pyropia yezoensis (Porphyra yezoensis; P yezoensis) is an edible marine red alga containing various biological macromolecules, such as sulfated polysaccharides, which have antioxidant, anti-inflammatory, antitumor and immunomodulatory activity (1)(2)(3)(4)(5). The anticancer bioactivities and applications of natural polysaccharides are of considerable interest to researchers and have been investigated using in vivo and in vitro models.…”