Development and characterization of SV40 immortalized rat parotid acinar cell lines

Abstract

Rat parotid salivary gland acinar cells were transfected by CaPO4 precipitation using a plasmid containing a replication-defective simian virus (SV40) genome. Out of 30 clonal cell lines, 2 were shown to have moderate to high levels of cytodifferentiation and salivary gland acinar cell function. Functional studies with the two cell lines indicated that the beta-adrenergic agonist (isoproterenol), vasoactive intestinal peptide prostaglandin E1, and forskolin were effective activators of intracellular cyclic adenosine 3':5'-cyclic monophosphate production. Phenylephrine, carbamylcholine, and UTP were effective in increasing inositol phosphate production and intracellular free calcium levels, whereas substance P was without affect. Utilizing indirect immunofluorescence analysis, both cell lines were shown to express the SV40 large T antigen. Electron microscopic evaluation documented moderate to high levels of cytodifferentiation with the maintenance of tripartite junctional complexes, cellular polarization, and presence of moderate amounts of secretory granules and rough endoplasmic reticulum. The two cell lines had doubling times of 22 and 36 h, respectively.

Synergistic suppression of apoptosis in salivary acinar cells by IGF1 and EGF

“…47 These cells demonstrated highly differentiated acinar cell markers and have maintained the b-adrenergic/ cyclic AMP signaling pathways. 47 Culture conditions and medium supplements have been previously described.…”

Low-level (gallium-aluminum-arsenide) laser irradiation of Par-C10 cells and acinar cells of rat parotid gland

“…We used two kinds of cells, Par-C10 derived from rat parotid acinar cell [14] (Par-C10; courtesy of Dr. David Quissell, Colorado University), and isolated rat parotid acinar cells in this study.…”

“…The characteristics of Par-C10 was described in an earlier report [14] by Quissell et al The Par-C10 cells were used to investigate cell proliferation and response to stress such as HSP. However, the cells do not produce amylase and are inappropriate for functional assay such as amylase releasing.…”

Conserved elements within first intron of aquaporin-5 (Aqp5) function as transcriptional enhancers

“…In transient transfection experiments, we used three different cell lines: MLE-15 (mouse lung epithelial) cells [13], Pa-4 (rat parotid acinar) cells [14], and NIH/3T3 (mouse embryo fibroblast) cells [15]. Both MLE-15 and Pa-4 cells express the Aqp5 gene [9], and were used in this study to represent lung and salivary gland, respectively, two AQP5-expressing organs.…”