Development and characterization of sodium alginate/bifidobacterium probiotic biohybrid material used in tissue engineering

Abstract

Mechanical properties" are crucial for biodegradable and/or nonbiodegradable materials used in tissue engineering applications. In this study, bio-hybrid films were produced by using both Bifidobacterium animalis subsp. lactis BB-12 probiotic strain and Bifidobacterium infantis in combination with sodium alginate (SA), which demonstrates biocompatibility and facilitated gelation properties. Bio-hybrid films were characterized by using different methods. Based on the spectroscopic and mechanical analysis, it was found that mechanical strength increased in films produced by adding Bifidobacterium infantis in SA while this increase was relatively lower as compared to those containing Bifidobacterium animalis subsp. lactis BB-12 as crosslinking ratio increases. Besides, bacteria contained in bio-hybrid films increased the percentage of amorphous zone of SA in SA/bacteria films, which reduced the crystallinity ratio. This indicated that crystalline chains contained in the structure of SA are degraded by bacteria.

Intratumoural microbiota: from theory to clinical application

“…Recent research has revealed that the pairing of Ecm-Alginate and Bifidobacterium BB-12 and Bifidobacterium Infantis strains can enhance the antibacterial and anti-inflammatory properties of the Bifidobacterium BB-12 and Bifidobacterium Infantis strains , thereby exhibiting some degree of anti-cancer activity. This phenomenon can be attributed to the interaction between the peptidoglycan on the extracellular polysaccharide cell membrane of the Bifidobacterium BB-12 and Bifidobacterium Infantis strains and Ecm-Alginate [ 130 ]. Zheng et al observed that colonization of the TME by engineered Salmonella induced the infiltration of rich immune cells (such as monocytes/macrophages and neutrophils) through TLR4 signal transduction, and Salmonella colonized in the tumor caused the functional activation of tumor macrophages with the M1 phenotype through the secretion of Vibrio vulnificus flagellin B (FlaB) and mutual reduction in the inhibitory activity of M2-like macrophages, thereby strengthening the effect of immunotherapy [ 131 ].…”

Preparation and characterization of amphiphilic, biodegradable, waterborne polyurethanes without using organic solvent and catalyst

“…However, it is crucial for biodegradable materials to possess good mechanical properties for applications in specialty fields, such as tissue engineering. 17 …”

“…However, it is crucial for biodegradable materials to possess good mechanical properties for applications in specialty elds, such as tissue engineering. 17 Synthetic polymers can be easily tailored, designed and chemically modied, as compared to natural polymers. In view of this, the synthesis, characterization, commercialization, and application of biodegradable synthetic polymers have received extensive attention.…”