Development and characterization of site specific target sensitive liposomes for the delivery of thrombolytic agents

Vaidya, Bhuvaneshwar; Nayak, Manasa K.; Dash, Debabrata; Agrawal, Govind P.; Vyas, Suresh P.

doi:10.1016/j.ijpharm.2010.10.028

Cited by 57 publications

(42 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescent labelled streptokinase (FITC-SK) was synthesized by using FITC labelling kit (KT-31, Bangalore Genei, Bengaluru, India) according to the manufacturer instruction. Synthesis of acylated peptide was described in our recent publication (Vaidya et al, 2011). Synthesized compound was characterized by FTIR and mass spectroscopy.…”

Section: Methodsmentioning

confidence: 99%

“…Liposomes were prepared by the procedure reported earlier by Ho et al (1986) and recently published by us (Vaidya et al, 2011). Briefly, DOPE and acylated peptide were dissolved in chloroform and solvent was evaporated using rotary vacuum evaporator (Strike 102, Steroglass, Perugia, Italy).…”

Section: Development Of Liposomesmentioning

confidence: 99%

“…Initially, platelets were adhered and activated to the collagen-coated coverslips as described earlier (Vaidya et al, 2011). Rhodamine 6G was used to stain the platelets adhered onto collagen-coated coverslips (Massberg et al, 1998).…”

Section: Platelet Binding Studies With Rgd-modified Liposomesmentioning

confidence: 99%

“…In a recent study, we have developed target-sensitive liposomes which were prepared by using moderate affinity peptide c(RGDfK) acylated with palmitic acid (Vaidya et al, 2011). In vitro results revealed that liposomes targeted activated platelets and destabilized thereafter interaction with activated platelets.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development and characterization of highly selective target-sensitive liposomes for the delivery of streptokinase: in vitro/in vivo studies

et al. 2014

Self Cite

View full text Add to dashboard Cite

Streptokinase is one of the most commonly used thrombolytic agents for the treatment of thromboembolism. Short half-life of the streptokinase requires administration of higher dose which results in various side effects including systemic haemorrhage due to activation of systemic plasmin. To increase the selectivity of the streptokinase and hence to reduce side effects, various novel carriers have been developed. Among these carriers, liposomes have been emerged as versatile carrier. In the present study, highly selective target-sensitive liposomes were developed and evaluated by in vitro and in vivo studies. Prepared liposomes were found to release streptokinase in vitro following binding with activated platelets. Intravital microscopy studies in thrombosed murine model revealed higher accumulation of liposomes in the thrombus area. In vivo thrombolysis study was performed in the human clot inoculated rat model. Results of the study showed that target-sensitive liposomes dissolved 28.27 ± 1.56% thrombus as compared to 17.18 ± 1.23% of non-liposomal streptokinase. Further, it was also observed that target-sensitive liposomes reduced the clot dissolution time as compared to streptokinase solution. Studies concluded that developed liposomes might be pragmatic carriers for the treatment of thromboembolism.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Development Of Liposomesmentioning

confidence: 99%

Section: Platelet Binding Studies With Rgd-modified Liposomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development and characterization of highly selective target-sensitive liposomes for the delivery of streptokinase: in vitro/in vivo studies

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…viadya et al developed rGd-peptide-conjugated liposomes loaded with streptokinase. 41 this in vitro drug release study showed that nearly 40% of the entrapped drug was released in 12 hours in phosphate buffered saline (ph 7.4). however, on incubation with activated platelets, about 90% of the drug was released within 45 minutes.…”

Section: Thrombolytic Therapy With Polymeric Nanocarriers and Immunolmentioning

confidence: 82%

Liposomes in Diagnosis and Treatment of Cardiovascular Disorders

Levchenko¹,

Hartner²,

Torchilin

2012

Methodist DeBakey Cardiovascular Journal

View full text Add to dashboard Cite

Immunoliposomes for Specific Drug Delivery

Calin¹

2012

Antibody‐Mediated Drug Delivery Systems

View full text Add to dashboard Cite

Development and characterization of site specific target sensitive liposomes for the delivery of thrombolytic agents

Cited by 57 publications

References 25 publications

Development and characterization of highly selective target-sensitive liposomes for the delivery of streptokinase: in vitro/in vivo studies

Development and characterization of highly selective target-sensitive liposomes for the delivery of streptokinase: in vitro/in vivo studies

Liposomes in Diagnosis and Treatment of Cardiovascular Disorders

Immunoliposomes for Specific Drug Delivery

Contact Info

Product

Resources

About