Development and characterization of simple sequence repeat markers in Zoysia japonica Steud.

Abstract

Simple sequence repeats (SSR) have several positive attributes as genetic markers when compared with other DNA‐based marker systems. Because of their successful use for genetic analysis in a number of plant species, they could be similarly useful tools for the genetic study of various Zoysia species. A genomic library enriched for the AG/TC motif was developed from Zoysia japonica cv. ‘Asagake’ and screened by using 5′‐biotin‐labeled oligonucleotides (AG20). As a result of sequencing analysis of 162 clones, a total of 119 clones were identified containing 8–35 SSR repeats. Thirty‐two primer pairs designed from these clones were evaluated for their ability to detect polymorphisms among six additional zoysiagrass cultivars. The average values of the observed heterozygosities and polymorphic information content for the individual loci were 0.57 and 0.69, respectively. These primers were successful in generating several informative cross‐amplification products in additional Zoysia species. The generation of informative SSR markers should be a valuable tool for identification, estimation of genetic diversity and construction of genetic linkage maps in Zoysia species.

Genetic relationship estimation in guineagrass (Panicum maximum Jacq.) assessed on the basis of simple sequence repeat markers

“…Tsuruta et al . (2005) reported that the screening of an enriched SSR library of zoysiagrass ( Zoysia japonica Steud.)…”

“…The average value of the number of putative alleles with 13 SSR was 14.9 and the average PD value was 0.859. The value of PD was large compared to zoysiagrass (Tsuruta et al . 2005), and Lolium perenne (Jones et al .…”

Construction of a high-density SSR marker-based linkage map of zoysiagrass (Zoysia japonica Steud.)

“…Of 1,088 SSR primer pairs, 1,000 SSR markers were developed from four SSR-enriched genomic libraries after testing one screening panel consisting of eight Zoysia clones for testing PCR amplifications (Cai et al 2005), and 88 were developed from genomic library enriched for AG/TC motif using Z. japonica cv. 'Asagake' by screening of six Zoysiagrass cultivars (Tsuruta et al 2005).…”

“…At present, SSR markers have been used to construct comprehensive genetic linkage maps in several forage crops, including red clover (Sato et al 2005), white clover (Jones et al 2003, Barrett et al 2004), alfalfa (Sledge et al 2005;Julier et al 2003), perennial ryegrass (Jones et al 2002), Italian ryegrass (Hirata et al 2006), and tall fescue . In Zoysia species, a number of SSR markers have been reported (Tsuruta et al 2005;Cai et al 2005). Although Cai et al (2005) developed 1,044 working SSR primers for zoysiagrass using an S 2 population in order to construct a genetic linkage map, only 170 SSR markers were mapped to the previously constructed AFLP map with many SSRs not located within any linkage group (Cai et al 2005).…”

The Highly Heterozygous Homoploid Turfgrass Zoysia matrella Displays Desirable Traits in the S1 Progeny

“…Similarly, winter tolerance decreased with decreasing of leaf width in zoysiagrass (Patton and Reicher, 2007). Simple sequence repeat markers allowed detection of homozygous and heterozygous loci for each DNA sample (Paige, 2010) and have been used to identify genetic diversity among Zoysia species (Tsuruta et al, 2005;Mantia et al, 2011;Kimball et al, 2013;Tanaka et al, 2016). According to previous studies, Z. matrella has intermediate morphological traits and overlapping geographical distribution with Z. japonica and Z. pacifica (Kitamura, 1967;Tanaka et al, 2015).…”