Development and characterization of <scp>EST</scp>‐<scp>SSR</scp> markers for Vitex negundo var. heterophylla (Lamiaceae)

Liu, Lele; Wang, Jingwen; Yin, Meiqi; Guo, Xiao; Cai, Yong; Du, Ning; Yu, Xiaona; Guo, Weihua

doi:10.1002/aps3.1209

Cited by 4 publications

(3 citation statements)

References 13 publications

(11 reference statements)

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“…And the results showed that there were significant relationships between epigenetics and genetic variation, epigenetics, and phenotypic variation. Liu et al (2019) have developed 14 pairs of expressed sequence tags and simple repeat sequence (EST-SSR) marker primers for this species, which provided useful resources for the study of reproduction and genetic ecology of V. negundo var. heterophylla.…”

Section: Introductionmentioning

confidence: 99%

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Mating strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae): A mixed mating system with inbreeding depression

Zhang,

Sun

et al. 2024

Ecology and Evolution

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Plant reproductive ecology is one of the research hotspots in ecology. With the increasing attention paid to the conservation of plant diversity, the research on reproductive characteristics and flowering biological characteristics of more species has attracted more attention. However, plant reproduction is affected by multiple interacting factors such as pollen limitation and resource availability. Vitex negundo var. heterophylla (Franch.) Rehder (Lamiaceae) is a significant species for water and soil conservation. Previous studies have revealed its mating system by the biological characteristics of flowering and SSR markers, but its reproductive strategies remain to be further studied. We evaluated reproductive success through artificial pollination to explore the reproductive characteristics of V. negundo var. heterophylla for the first time. From the results of fruit set, there is a mixed mating system dominated by outcrossing in V. negundo var. heterophylla accompanied by self‐compatibility, and it cannot carry out autonomous selfing. Our data show the pollinator‐mediated interaction in the success of reproduction, whereas the effect of anemophily is very weak. And the seed germination rate of inbred line progenies was lower than that of hybrid progenies, which is suspected to be caused by inbreeding depression. The research will provide scientific information for the protection and conservation of V. negundo var. heterophylla from the point of view of reproduction. In sum, the results are necessary to protect animal vectors in the background of insect decline.

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Section: Introductionmentioning

confidence: 99%

“…Liu et al. ( 2019 ) have developed 14 pairs of expressed sequence tags and simple repeat sequence (EST‐SSR) marker primers for this species, which provided useful resources for the study of reproduction and genetic ecology of V. negundo var. heterophylla .…”

Section: Introductionmentioning

confidence: 99%

Mating strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae): A mixed mating system with inbreeding depression

Zhang,

Sun

et al. 2024

Ecology and Evolution

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“…Through various extraction techniques as well as using different solvents it was estimated that the luteolin content in leaves can be attained to its maximum by reflux technique and using methanol as solvent. From the leaves genomic DNA was extracted using the cetyltrimethylammonium bromide method and 12,075 simple sequence repeat markers were detected 87 . The flavonoid extract of flower buds showed the presence of phenols, naphthalene, 2,3-dihydrobenzofuran, 5-hydroxy-3,6,7,3' ,4'-pentamethoxy flavones, phenol-2,4-Bis flavones through GCMS analysis 88 .…”

Section: Clinical Studiesmentioning

confidence: 99%

A Systematic Review on the Pharmacological Prospectives of Vitex negundo in Ayurveda Drug Research

Devipriya,

Mohan,

Mohanan

et al. 2023

JNR

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In Ayurveda, there are many formulations containing Vitex negundo which is indicated in specific pathogenic and non-pathogenic condition. Hence this review attempted to explore the data of research at a glimpse. Peer reviewed and published data from PubMed had been selected for the same. The PubMed database (2009 to 2019) were searched using the keyword ‘Vitex negundo’, ‘Ayurveda’ with Boolean operator ‘AND’. A total of 45 free full text articles were retrieved from 57 articles with specific inclusion criteria. All the 45 studies were categorized into in silico studies, analytical studies, in vitro studies, in vivo studies and clinical studies. Then the data such as the type and design of research, the techniques used, and sample size were categorized into tables and the results were attained through thorough reading and analysis. A descriptive method is adopted for the review and quality aspects of the data were not taken into consideration. Compounds namely, acerosin and thymol were studied in in silico as an inhibitor for mTOR and on skin diseases respectively. Analytical studies explored the presence of compounds such as estriol, angnuside, phydroxybenzoicacid etc. Pharmacological activities such as anti-bacterial, anti-cancerous, anti-tubercular were studied in vitro and in vivo. A clinical study on Nirgundi khanavati in gridrasi (sciatica) is found to be effective in reducing the pain threshold. The phytochemical research of the drug were also proven against carcinoma. There is a high scope for research for more clinical studies and standardization with advanced analytical procedures and marker compounds.

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Studies on reproductive strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) based on morphological characteristics and SSR markers

Sun

Wang

Cui

et al. 2020

Ecology and Evolution

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Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) is an important tree species for soil and water conservation, yet the reproductive ecology of this species remains to be elucidated. To investigate the reproductive traits of V. negundo var. heterophylla, the phenology, morphological characteristics (a suite of characters was assessed: floral morphology, nectar production, pollen viability, and stigma receptivity) and mating system of this species were systematically revealed for the first time in this study. Phenological observations, morphological measurements, and nectar production analysis were conducted during anthesis. Pollen viability and stigma receptivity at different flowering stages were measured by biochemical methods. Finally, genetic analysis based on SSR markers was used to reveal the mating system; outcrossing index and pollen‐ovule ratio were also calculated to help analysis. V. negundo var. heterophylla showed several obvious characteristics of outcrossing, such as abundant and attractive flowers, secreting nectar, and emitting scent. In addition, mechanisms such as homogamy and a short anther‐stigma distance that can promote self‐fertilization were also identified in this species. The coexistence of selfing and outcrossing characteristics demonstrates a predominantly outcrossed mixed mating system (outcrossing rate, t = 95%). The scientific information provided by this study may contribute to conservation of V. negundo var. heterophylla from a reproductive perspective.

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Development and characterization of EST‐SSR markers for Vitex negundo var. heterophylla (Lamiaceae)

Cited by 4 publications

References 13 publications

Mating strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae): A mixed mating system with inbreeding depression

Mating strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae): A mixed mating system with inbreeding depression

A Systematic Review on the Pharmacological Prospectives of <i>Vitex negundo</i> in <i>Ayurveda</i> Drug Research

Studies on reproductive strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) based on morphological characteristics and SSR markers

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