Development and characterization of polymorphic microsatellite loci in the sea cucumber Holothuria leucospilota

Abstract

ABSTRACT. Holothuria leucospilota is a tropical holothurian species that is widely distributed in the tropical and sub-tropical India-Western Pacific Region. Eight polymorphic microsatellite loci were developed from H. leucospilota by using the protocol fast isolation by amplified fragment length polymorphism of sequences containing repeats and tested in 30 individuals from Hainan Island in China. The number of alleles was 2-6 and polymorphism information content ranged from 0.371-0.694. The levels of expected and observed heterozygosities varied from 0.3913-0.6701 and from 0.1154-0.7000, respectively. No significant linkage disequilibrium was detected for any pairwise combination of loci. Only loci YZHS1-42 deviated from HardyWeinberg equilibrium. These polymorphic microsatellite loci may be useful for germplasm conservation of H. leucospilota.

UPLC-Q-TOF/MS-based metabonomics reveals mechanisms for Holothuria leucospilota polysaccharides (HLP)-regulated serum metabolic changes in diabetic rats

“…Holothuria leucospilota is an edible sea cucumber widely distributed in the Indo-Pacific region ( Dai et al, 2015 ). The main components of extracts from Holothuria leucospilota polysaccharide (HLP) are primarily N -acetylgalactosamine, fucose, and glucuronic acid ( Yuan et al, 2019 , Yuan et al, 2019 ).…”

Sex or Fission? Genetics Highlight Differences in Reproductive Strategies of Two Sympatric Fissiparous Sea Cucumber Species in Reunion Island (Southwestern Indian Ocean)

“…The fission rates for populations of H. leucospilota, estimated thanks to a visual census of individuals undergoing fission, ranges between 5% in Reunion Island [23] and 28% in Australia [28]. Although H. leucospilota is one of the most common sea cucumber species, only two studies have investigated its genetic diversity [29,30], and no study has ever evaluated the genetic structure and clonal propagation of its populations using genetic tools. The number of individuals of S. chloronotus performing fission has been estimated to 17% at Reunion Island [24], using the same method as for H. leucospilota [23].…”