Development and characterization of normal colonic epithelial cell lines derived from normal mucosa of patients with colon cancer

Stauffer, Jay S.; Manzano, L.A.; Balch, Glen C.; Merriman, Ronald L.; Tanzer, Lee R.; Moyer, Mary Pat

doi:10.1016/s0002-9610(99)80135-4

Cited by 27 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1c-1f). In agreement with previous investigations, the three cell cultures we have developed express CEA [5,7], CSA [5], keratin [6,7], and mucin [7], all of which are considered characteristic of colon epithelium.…”

Section: Discussionsupporting

confidence: 91%

An Atraumatic Method to Establish Human Colon Carcinoma in Long-Term Culture

Mohammadpour¹,

Hall²,

Pardini³

et al. 1999

Journal of Surgical Research

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 91%

An Atraumatic Method to Establish Human Colon Carcinoma in Long-Term Culture

Mohammadpour¹,

Hall²,

Pardini³

et al. 1999

Journal of Surgical Research

View full text Add to dashboard Cite

“…This cell line was derived from the wide-margin normal mucosa of a colorectum resected to remove a rectal adenocarcinoma (17). Importantly for our studies, NCM356 cells express a very low level of CBS protein when compared to either colon cancer specimens or colon cancer-derived cell lines (18).…”

Section: Resultsmentioning

confidence: 99%

“…Importantly for our studies, NCM356 cells express a very low level of CBS protein when compared to either colon cancer specimens or colon cancer-derived cell lines (18). Also, NCM356 cells do not grow in a soft agar assay, a surrogate for anchorage-independent growth, and are non-tumorigenic when xenografted into immunocompromised mice (17,19). Using Next Generation sequencing, we confirmed the existence of a mutant TP53 gene reported by Stauffer, et al (17), and identified additional functional mutations in the KRAS and APC genes (Supplemental Table S1).…”

Section: Resultsmentioning

confidence: 99%

“…Also, NCM356 cells do not grow in a soft agar assay, a surrogate for anchorage-independent growth, and are non-tumorigenic when xenografted into immunocompromised mice (17,19). Using Next Generation sequencing, we confirmed the existence of a mutant TP53 gene reported by Stauffer, et al (17), and identified additional functional mutations in the KRAS and APC genes (Supplemental Table S1). These data suggest that the NCM356 cell line exhibit the characteristics of a late adenoma.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Upregulation of Cystathionine-β-Synthase in Colonic Epithelia Reprograms Metabolism and Promotes Carcinogenesis

et al. 2017

View full text Add to dashboard Cite

The transsulfuration enzyme cystathionine-β-synthase (CBS) and its product hydrogen sulfide (H2S) are aberrantly upregulated in colorectal cancers, where they contribute to tumor growth and progression by both autocrine and paracrine mechanisms. However, it is unknown whether the CBS/H2S axis plays a role in colorectal carcinogenesis. Here, we report upregulation of CBS in human biopsies of precancerous adenomatous polyps and show that forced upregulation of CBS in an adenoma-like colonic epithelial cell line is sufficient to induce metabolic and gene expression profiles characteristic of colorectal cancer cells. Differentially expressed metabolites (65 increased and 20 decreased) clustered into the glycolytic pathway, nucleotide sugars, intermediates of the pentose phosphate pathway, and lipogenesis, including primarily phospholipids, sphingolipids, and bile acids. CBS upregulation induced broad changes in the NCM356 cell transcriptome with over 350 differentially expressed genes. These genes overlapped significantly with gene sets related to glycolysis, hypoxia, and a colon cancer cell phenotype, including genes regulated by NF-κB, KRAS, p53, and Wnt signaling, genes downregulated after E-cadherin knockdown, and genes related to increased extracellular matrix, cell adhesion, and epithelial-to-mesenchymal transition. The CBS-induced switch to an anabolic metabolism was associated with increased NCM356 cell bioenergetics, proliferation, invasion through Matrigel, resistance to anoikis, and CBS-dependent tumorigenesis in immune compromised mice. Genetic ablation of CBS in CBS heterozygous mice (CBS+/−) reduced the number of mutagen-induced aberrant colonic crypt foci. Taken together, these results establish that activation of the CBS/H2S axis promotes colon carcinogenesis.

show abstract

“…To address whether acquisition of the CCK 2 receptor is sufficient to convert a non‐tumorigenic, gastrin‐producing colorectal cell into a tumorigenic cell, we employed the NCM356 epithelial cell line, derived from the normal colon mucosa wide margin resection of a patient with rectal adenocarcinoma 19. The NCM356 cells express colon epithelial antigens, including cytokeratin, villin and mucin.…”

mentioning

confidence: 99%

Prognostic factors in nonoperative therapy for chronic symptomatic calcific tendinitis of the shoulder

Ogon

Suedkamp

Jaeger

et al. 2009

Arthritis & Rheumatism

106

View full text Add to dashboard Cite

Objective. To define prognostic factors in chronically symptomatic patients with calcific tendinitis of the shoulder.Methods. We evaluated 420 patients (488 shoulders) in the context of a prospective cohort study. Epidemiologic data were assessed. The radiographic and sonographic appearance of the calcific deposits was classified. The mean period of nonoperative therapy was 4.4 years (range 0.5-13.7 years). After referral to our institution, standardized nonoperative therapy was continued for a minimum of 3 months. Failure of nonoperative therapy was defined as the persistence of symptomatic calcific tendinitis of the shoulder after a minimum of 6 months. Prognostic factors (determined at P < 0.05 by chi-square test) were analyzed by logistic regression.Results. Of the 420 patients, 269 (64%) were women, 151 (36%) were men. The mean age of the patients was 51.3 years (range 28-84 years). Occurrence of calcific tendinitis of the shoulder was unilateral in 84% and bilateral in 16%. Gärtner type I calcific deposits were found in 37%, type II in 32%, and type III in 31%. Failure of nonoperative therapy was observed in 114 patients (27%). Negative prognostic factors were bilateral occurrence of calcific tendinitis of the shoulder, localization to the anterior portion of the acromion, medial (subacromial) extension, and high volume of the calcific deposit. Positive prognostic factors were a Gärt-ner type III deposit and a lack of sonographic sound extinction of the calcific deposit.Conclusion. Our findings demonstrate the existence of prognostic factors in the nonoperative treatment of chronic symptomatic calcific tendinitis of the shoulder. Guidelines for optimal treatment can be implemented according to these factors to avoid a longterm symptomatic disease course.

show abstract

Development and characterization of normal colonic epithelial cell lines derived from normal mucosa of patients with colon cancer

Cited by 27 publications

References 17 publications

An Atraumatic Method to Establish Human Colon Carcinoma in Long-Term Culture

An Atraumatic Method to Establish Human Colon Carcinoma in Long-Term Culture

Upregulation of Cystathionine-β-Synthase in Colonic Epithelia Reprograms Metabolism and Promotes Carcinogenesis

Prognostic factors in nonoperative therapy for chronic symptomatic calcific tendinitis of the shoulder

Contact Info

Product

Resources

About