Development and characterization of nitrogen and phosphorus use efficiency responsive genic and miRNA derived SSR markers in wheat

Sagwal, Vijeta; Sihag, Pooja; Singh, Yogita; Mehla, Sheetal; Kapoor, Prexha; Balyan, Priyanka; Kumar, Anuj; Mir, Reyazul Rouf; Dhankher, Om Parkash; Kumar, Upendra

doi:10.1038/s41437-022-00506-4

Cited by 15 publications

(9 citation statements)

References 71 publications

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“…The availability of genetic and genomic resources will pave the way for the discovery of useful/stable/major genes/QTLs/markers for seed micronutrients in chickpea through a variety of approaches. A variety of molecular markers have been developed in almost all crop plants and these markers have been used for variety of purposes including the study of genetic diversity, study of population structure and also in gene discovery programs 43 – 51 ). However, despite the abundance of different types of markers available, SNPs have witnessed exponential utilization for a variety of diverse applications since their discovery 52 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association analysis to delineate high-quality SNPs for seed micronutrient density in chickpea (Cicer arietinum L.)

Fayaz

Tyagi

Wani

et al. 2022

Sci Rep

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Chickpea is the most important nutrient-rich grain legume crop in the world. A diverse core set of 147 chickpea genotypes was genotyped with a Axiom(®)50K CicerSNP array and trait phenotyped in two different environments for four seed micronutrients (Zn, Cu, Fe and Mn). The trait data and high-throughput 50K SNP genotypic data were used for the genome-wide association study (GWAS). The study led to the discovery of genes/QTLs for seed Zn, Cu, Fe and Mn, concentrations in chickpea. The analysis of seed micronutrient data revealed significant differences for all four micronutrient concentrations (P ≤ 0.05). The mean concentrations of seed Zn, Cu, Fe and Mn pooled over the 2 years were 45.9 ppm, 63.8 ppm 146.1 ppm, and 27.0 ppm, respectively. The analysis of results led to the identification of 35 SNPs significantly associated with seed Zn, Cu, Fe and Mn concentrations. Among these 35 marker-trait associations (MTAs), 5 were stable (consistently identified in different environments), 6 were major (explaining more than 15% of the phenotypic variation for an individual trait) and 3 were both major and stable MTAs. A set of 6 MTAs, MTAs (3 for Mn, 2 for Fe, and 1 for Cu) reported by us during the present study have been also reported in the same/almost same genomic regions in earlier studies and therefore declared as validated MTAs. The stable, major and validated MTAs identified during the present study will prove useful in future chickpea molecular breeding programs aimed at enhancing the seed nutrient density of chickpea.

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Section: Discussionmentioning

confidence: 99%

Genome-wide association analysis to delineate high-quality SNPs for seed micronutrient density in chickpea (Cicer arietinum L.)

Fayaz

Tyagi

Wani

et al. 2022

Sci Rep

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“…It was discovered that the interaction between N and P was substantial. These findings are backed up by [19] who found that applying mineral fertilizers at the proper time considerably improved wheat uptake of nutrients like P.…”

Section: P Uptakementioning

confidence: 93%

Effect of Nitrogen and Phosphate Fertilizer on Phosphorus Uptake and Phosphorus Use Efficiency in Some of Wheat Varieties (T. aestivum and T. durum)

Sharma¹,

Elanchezhian

Gurjar

et al. 2023

IJECC

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A field experiment was carried out in a subtropical region of India. The main purpose of this experiment was to grow different wheat genotypes under different nutrient doses and find out which genotype performed better in terms of yields and other attributes under nutrient constraints. Nine varieties of wheat (T. aestivum and T. durum L.) were selected and grown as test crops in the current investigation, adopting a split plot design that was replicated three times, with nutrient dose as the main plot and varieties as sub treatments. In the treatmentT1 = control, T2 = 100% (N+P+K), T3 = 50% N+100% (P+K), and T4 = 50% P+100% N+K, there are 36 plots in a block (9 varieties x 4 fertilizer N and P treatments). Of the nine varieties grown under normal N and P conditions, varieties NARMADA 14 and GW 366 had the highest P content in grain and straw. GW366 (10.93 kg/ha) had the highest average total P uptake, followed by NARMADA14 (10.90 kg/ha). Under normal doses of N and P, the variety HI 1531 (15.25%) had the highest apparent recovery of P, followed by NARMADA14 (15.15percent).Across all fertilizer N and P dosages, the highest average agronomic efficiency for P was found in GW366 (40.09%), followed by LOK1 (37.93%). NARMADA 14 and GW366 have the highest P content in grain and straw of the nine varieties grown under normal nitrogen conditions. Agronomic use efficiency and physiological use efficiency were generally substantially higher when computed on a P basis than they were when calculated on an N basis. P fraction concentrations in black soil varied, but they responded similarly to crop species and N sources. The N supply had an impact on the amounts of P taken from the soil. In P-deficient soils, the use of an appropriate N source and the selection of the right crop species may boost crop yield and plant P uptake.

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“…The study was carried out in the screen house of the Department of Molecular biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar. Wheat genotypes used for the study were selected based on their use efficiency for N and P as reported in our previous study (Sagwal et al, 2022). Seeds of four wheat genotypes-WH147 (high NUE), DBW16 (low NUE), HD2967 (low PUE) and WH1100 (high PUE) were sown in a completely randomized block design (CRD) in triplicates in earthen pots.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Many studies have shown the effect of N and P fertilizers on wheat growth, yield, and physiological parameters such as total chlorophyll content (TCC) and net photosynthetic rate (NPR) (Bosquet et al, 2009;Ghafoor et al, 2021;El Sobky, 2021;Fontana et al, 2021). To adapt to the limiting nutrient conditions, plants have evolved several complicated physiological and biochemical responses like inhibition of primary root elongation, increase in density and growth of lateral roots, decrease in CO2 assimilation rate, and photosynthesis (Li et al, 2018;Prathap et al, 2022, Sagwal et al, 2022. The molecular mechanisms regulating these responses have involved the genes which help in the uptake, transport, localization, and accumulation of these limiting nutrients (Aung et al, 2006;Zhao et al, 2011;He et al, 2014;Olsen et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Physiological traits and expression profile of genes associated with nitrogen and phosphorous use efficiency in wheat

Sagwal

Kumar

Sihag

et al. 2023

Preprint

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Background Nitrogen (N) and phosphorous (P) play a very important role in growth and development of wheat as well as major constituents of biological membrane. To meet the plant nutritional demand these nutrients applied in the form of fertilizers. But plant can utilize only half of the applied fertilizer whereas rest is lost thorough surface run off, leaching and volatilization. Thus, to overcome the N/P loss we need to elucidate the molecular mechanism behind the N/P uptake. Methods In our study, we used DBW16 (low NUE), and WH147 (high NUE) wheat genotypes under different doses of N, whereas HD2967 (low PUE) and WH1100 (high PUE) genotypes were studied under different doses of P. To check the effect of different doses of N/P, the physiological parameters like Total chlorophyll content, net photosynthetic rate, N/P content, and N/PUE of these genotypes were calculated. In addition, gene expression of various genes involved in N/P uptake, utilization, and acquisition were studied by quantitative real time- PCR. Results Statistical analysis revealed a lower percent reduction in TCC, NPR, N/P content in N/P efficient wheat genotypes (WH147 & WH1100). A significant increase in relative fold expression of genes under low N/P concentration was observed in N/P efficient genotypes as compared to N/P deficient genotypes. Conclusion Significant differences in physiological data and gene expression among N/ P efficient and deficient wheat genotypes could be useful for future improvement of N/P use efficiency.

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Development and characterization of nitrogen and phosphorus use efficiency responsive genic and miRNA derived SSR markers in wheat

Cited by 15 publications

References 71 publications

Genome-wide association analysis to delineate high-quality SNPs for seed micronutrient density in chickpea (Cicer arietinum L.)

Genome-wide association analysis to delineate high-quality SNPs for seed micronutrient density in chickpea (Cicer arietinum L.)

Effect of Nitrogen and Phosphate Fertilizer on Phosphorus Uptake and Phosphorus Use Efficiency in Some of Wheat Varieties (T. aestivum and T. durum)

Physiological traits and expression profile of genes associated with nitrogen and phosphorous use efficiency in wheat

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