Development and characterization of nineteen polymorphic microsatellite loci from seaside alder, Alnus maritima

Lance, Stacey L.; Jones, Kenneth L.; Hagen, Cris; Glenn, Travis C.; Jones, James M.; Gibson, J. Phil

doi:10.1007/s10592-009-9851-y

Cited by 10 publications

(6 citation statements)

References 9 publications

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“…DNA was extracted from Southern Leopard frog tissue using silica‐binding techniques (Lance et al., ) and from Dwarf salamander tissue using phenol chloroform extractions (Sambrook, Fritsch, & Maniatis, ). Southern Leopard frog DNA samples were screened at 11 microsatellite loci (Rasp03, Rasp07, Rasp09, Rasp10, Rasp13, Rasp17, Rasp37, Rasp45, Rasp50, Rasp53, and Rasp55) (McKee, Lance, Jones, Hagen, & Glenn, ) and Dwarf salamander DNA samples at 12 microsatellite loci (Mckee, Lance, Jones, Hagen, & Glenn, ) using a 3730xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Habitat predictors of genetic diversity for two sympatric wetland‐breeding amphibian species

McKee

Maerz

Smith

et al. 2017

Ecology and Evolution

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Population genetic diversity is widely accepted as important to the conservation and management of wildlife. However, habitat features may differentially affect evolutionary processes that facilitate population genetic diversity among sympatric species. We measured genetic diversity for two pond‐breeding amphibian species (Dwarf salamanders, Eurycea quadridigitata; and Southern Leopard frogs, Lithobates sphenocephalus) to understand how habitat characteristics and spatial scale affect genetic diversity across a landscape. Samples were collected from wetlands on a longleaf pine reserve in Georgia. We genotyped microsatellite loci for both species to assess population structures and determine which habitat features were most closely associated with observed heterozygosity and rarefied allelic richness. Both species exhibited significant population genetic structure; however, structure in Southern Leopard frogs was driven primarily by one outlier site. Dwarf salamander allelic richness was greater at sites with less surrounding road area within 0.5 km and more wetland area within 1.0 and 2.5 km, and heterozygosity was greater at sites with more wetland area within 0.5 km. In contrast, neither measure of Southern Leopard frog genetic diversity was associated with any habitat features at any scale we evaluated. Genetic diversity in the Dwarf salamander was strongly associated with land cover variables up to 2.5 km away from breeding wetlands, and/or results suggest that minimizing roads in wetland buffers may be beneficial to the maintenance of population genetic diversity. This study suggests that patterns of genetic differentiation and genetic diversity have associations with different habitat features across different spatial scales for two syntopic pond‐breeding amphibian species.

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Section: Methodsmentioning

confidence: 99%

“…DNA was extracted from Southern Leopard frog tissue using silicabinding techniques (Lance et al, 2009) and from Dwarf salamander tissue using phenol chloroform extractions (Sambrook, Fritsch, & Maniatis, 1989 were rerun and analyzed at each locus to estimate genotyping error rates (Table S1).…”

Section: Microsatellite Marker Development and Analysismentioning

confidence: 99%

Habitat predictors of genetic diversity for two sympatric wetland‐breeding amphibian species

McKee

Maerz

Smith

et al. 2017

Ecology and Evolution

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“…During the current study of Alnus nitida four morphological traits of 50 genotypes of Alnus nitida were evaluated (Lance et al, 2009) to find out their correlation. During the present study petiole size was found strongly significant (0.97 ** ) with leaf size, whereas catkin size, was found negative correlate with leaf size (-0.10), nut size was found significantly correlated with leaf size, and petiole size with the value of (0.15*), while found negatively (-0.27) correlated with catkin size (Table 5).…”

Section: Correlation Analysis Of 4 Morphological Traits Of Alnus Niti...mentioning

confidence: 99%

Genetic Diversity of Alnus nitida Reported from Dir Lower, Khyber Pakhtunkhwa, Pakistan

Khan¹,

Majid²,

Nisar³

et al. 2022

SJA

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A lnus nitida belongs to the family Betulaceae, is a deciduous tree. The species is monoecious, flowers are either male or female. Alnus nitida (Spach) Endl is commonly known as Seril in Kashmir and Sharol in Punjabi, while in Pashto it is called Geiray. Its vernacular name is alder. It is a deciduous woody tree about 20 meters tall. It is native to the Himalayas and found in Kashmir to Western Nepal at an elevation of 1000 to 2700 m (Mandak et al., 2016). Young shoots are velvety and pubescent, becoming glabrous when old. Leaves are ovate to elliptic-ovate, petiole 1-4 cm long, glabrous to pubescent. Male flowers are borne in catkins, up to 19cm long. Female flowers arise in erect mostly solitary wood cones 3-3.57 cm x 1.28 cm, bract broadly ovate. Nut sizes are 2.5-4.0 mm long, fringed by the thin arrow and more or less leathery wings. Alnus nitida species comprised diarylheptanoids which is a group of natural compounds containing 1, 77-diphenylheptane skeleton and have diverse remedial effects such as anti-inflammatory, Abstract | Morphological characterization is important in determining the genetic variation of genotypes among different plant species and its knowledge help the breeders and farmers to select the best variety. Alnus nitida is one of the native and most important plants in District Dir Lower, Khyber Pakhtunkhwa, Pakistan, mostly used for medicinal purposes. In the present study, total 50 genotypes of Alnus nitida were collected from Dir lower and evaluated for morphological traits (leaf, petiole, nut, and catkin size). A significant level of variations was observed in the size of the leaf (10.22%), petiole (24.84%), catkin (9.19%), and nut (3.08%). There is a significant correlation between petiole size and leaf size, which is an important nutritional parameter that could be used successfully in future breeding programs. Based on cluster analysis all the genotypes were divided into two main lineages; lineage A which is further divided into 3 clusters (C1, C2, and C3), and lineage B, further divided into 2 clusters (C4 and C5). The principal component analysis estimated the total variations in the range of 51.43 to 100% with an Eigenvalue of 0.25. It was concluded from the results that Alnus nitida L. genotypes available in Pakistan have come from a narrow gene pool and such types of variations can be exploited to develop new varieties with desirable traits.

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“…Total genomic DNA was extracted from frozen leaf material according to an adjusted ATMAB DNA extraction method [77]. In total, DNA was successfully extracted from 189 samples, which were further used for DNA analysis with 15 microsatellite markers (nSSR): A2 [78], A7 [79]; A10 [80]; A22 [81]; A26 [82]; A35, A37 and A38 [83]; Ag01, Ag05, Ag09, Ag10, Ag13, Ag20, and Ag30 [84] (Table S1). Polymerase chain reactions were performed in two multiplexes (A and B) in a final volume of 15 μL containing 1.0 μL of genomic DNA (about 10 ng), 5.5 μL H2O RNase-free water, 7.5 μL of Qiagen Multiplex PCR Master Mix 2×, and 1.0 μL of 10× primer mix (thermal cycler from GeneAmp ® PCR System 9700, Applied Biosystems, Foster City, CA, USA).…”

Section: Dna Extraction and Microsatellite Genotypingmentioning

confidence: 99%

Identification of Alnus glutinosa L. and A. incana (L.) Moench. Hybrids in Natural Forests Using Nuclear DNA Microsatellite and Morphometric Markers

Jurkšienė

Tamošaitis

Kavaliauskas

et al. 2021

Forests

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Two alder species (Alnus glutinosa and A. incana) have overlapping distribution, naturally occur in Lithuania, and are considered ecologically and economically important forest tree species. The objective of our study was to estimate the likelihood of spontaneous hybridizations between native alders in natural stands of Lithuania based on leaf morphology and nuclear microsatellite markers. The sampled trees were assigned to the three taxonomic groups of A. glutinosa, A. incana, and potential hybrids based on the leaf and bark morphological traits. The genetic differentiation and potential hybridization between these three groups was tested based on 15 nSSR markers. We identified studied Alnus spp. individuals as pure species and hybrids. Two microsatellite loci were reported as discriminating well between these species. We concluded that our results showed the highest likelihood of two genetic group structures, a clear genetic differentiation between the morphology-based groups of A. glutinosa and A. incana, and rather variable likelihood values in the putative hybrid group. The results provide important implications for genetic conservation and management of Alnus spp.

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Development and characterization of nineteen polymorphic microsatellite loci from seaside alder, Alnus maritima

Cited by 10 publications

References 9 publications

Habitat predictors of genetic diversity for two sympatric wetland‐breeding amphibian species

Habitat predictors of genetic diversity for two sympatric wetland‐breeding amphibian species

Genetic Diversity of Alnus nitida Reported from Dir Lower, Khyber Pakhtunkhwa, Pakistan

Identification of Alnus glutinosa L. and A. incana (L.) Moench. Hybrids in Natural Forests Using Nuclear DNA Microsatellite and Morphometric Markers

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