Development and characterization of new microsatellite markers for ginseng (Panax ginseng C. A. Meyer)

Ma, Kyung‐Ho; Dixit, Anupam; Kim, Young-Chang; Lee, Dong−Yun; Kim, Tae‐San; Cho, Eun-Gi; Park, Yong‐Jin

doi:10.1007/s10592-007-9284-4

Cited by 44 publications

(32 citation statements)

References 4 publications

(6 reference statements)

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“…Characterization of SSR-containing clones in the enriched library with reference to relative abundance of various repeat motifs revealed predominance of tri-nucleotide SSR (53.02%) over di-nucleotide SSR (37.21%). This is a feature common to other plant genomes, as several recent surveys on distribution patterns of various SSRs in genomic sequences of several plant species, including Arabidopsis (Morgante et al, 2002), foxtail millet (Jia et al, 2009), garlic (Ma et al, 2009), ginseng (Ma et al, 2007), Italian millet [our unpublished results], Medicago, Lotus, and Glycine (Mun et al, 2006), mung bean (Gwag et al, 2006), rice (Goff et al, 2005), and sesame (Dixit et al, 2005), have indicated that tri-nucleotide repeats are the most common type of SSR in plants. Occurrence of the AG/GA class of repeat motif among di-nucleotide repeats is consistent with reports from other plant species (Langercrantz et al, 1993;Morgante and Oliveiri, 1993;Powell et al, 1996, our unpublished results).…”

Section: Discussionmentioning

confidence: 87%

Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)

et al. 2010

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Millets such as proso millet have excellent nutritional properties and could become a basic resource for crop breeding programs and food diversification. In this study, 25 polymorphic microsatellite markers were developed and characterized through construction of an SSR-enriched library from genomic DNA of proso millet (Panicum miliaceum L.). In total,110 alleles were detected, with an average of 4.4 alleles per locus. Values of major allele frequency (MAF) and expected heterozygosity (HE) ranged from 0.36 to 0.98 (mean = 0.73) and from 0.04 to 0.74 (mean = 0.37), respectively. The mean genetic similarity coefficient was 0.3711, indicating that among 50 accessions of proso millet there was wide genetic variation. The newly developed microsatellite markers should be useful tools for assessing genetic diversity, understanding population structure, and breeding of proso millet.

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Section: Discussionmentioning

confidence: 87%

Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)

et al. 2010

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“…하지만 현재까지 인삼의 SSR 마커 개발 연구는 시작 단계 에 있고 일부 국내 연구진을 중심으로 약 80여종이 SSR 마 커가 개발되었으며, 주로 국내·외 수집종과 지역 재배종에 대한 유전적 다양성 평가와 국내 육성 품종 판별에 국한된 연 구가 진행되어왔다 (Ma et al, 2007;Kim et al, 2007;Nguyen et al, 2010 3. 유전자형 분석 마커에 대한 number of allele (N A ), major allele frequency…”

Section: 따라서 국내·외에서 다양한 유전자원을 수집하고 이들 유 전자원을 식물학적 분류 외부 형태적 특성 및 유전unclassified

Analysis of Genetic Polymorphism of Korean Ginseng Cultivars and Foreign Accessions using SSR Markers

Bang¹,

Jo²,

Chung³

et al. 2011

Korean Journal of Medicinal Crop Science

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: In this study, simple sequence repeat (SSR) analyses were utilized for evaluation of genetic diversity and discrimination of 17 accessions. Five cultivars, which were developed from Korea, and 12 foreign accessions, which were collected from China, Japan, Russia and USA, were evaluated by nine markers out of 22 SSR markers. A total of 39 alleles were detected, ranging from 2 to 8, with an average of 4.3 alleles per locus. The expected heterozygosity and PIC values were 0.627 and 0.553, with a range from 0.21 (GB-PG-078) to 0.76 (GB-PG-142) and from 0.19 (GB-PG-078) to 0.70 (GB-PG-142), respectively. Four makers out of nine SSR markers, GB-PG-026, GB-PG-043, GB-PG-142 and GB-PG-177, were selected as key factors for discrimination of Korean ginseng cultivars and foreign accessions. All of Korean ginseng cultivars and foreign accessions were individually by the combination of four SSR markers. Consequently, the SSR markers developed in this study may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and foreign accessions.

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“…Besides, many researchers have tried to identify ginseng species and varieties using SSR markers Ma et al 2007;Nguyen et al 2010;Bang et al 2011aBang et al , 2011b. SSR markers are important for several types of research, including the assessment of genetic diversity, development of genetic maps, comparative genomics, marker-assisted selection, and other plant breeding fields.…”

Section: Introductionmentioning

confidence: 99%

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

Um¹,

Mitsugi²,

Kim³

et al. 2016

Plant Breed. Biotech

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2016 (February) ABSTRACT Panax ginseng has been one of the most important herbal medicines used in Eastern Asia. Recently, various molecular markers have been developed to authenticate Panax species, but these markers cannot differentiate the exact varieties or variants of Korean ginseng cultivars. In this study, six cultivars of Korean ginseng (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng were differentiated by simple sequence repeat (SSR) marker development. Specific primer sets were designed for the 54 candidate sequences containing SSRs that were predicted. Finally, eight polymorphic SSR loci were developed. DNA fragment analysis was performed using fluorescence-labelled primers for the amplicons. Reproducibility tests were carried out using multiple samples of Korean ginseng cultivars and Panax species. Eight primer sets (PgSSR07, PgSSR08, PgSSR09, PgSSR17, PgSSR37, PgSSR40, PgSSR51, and PgSSR53) showing polymorphism were used for phylogenetic relationship analysis. Consequently, six Korean ginseng cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng could be identified using the combination of SSR markers discovered.

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Development and characterization of new microsatellite markers for ginseng (Panax ginseng C. A. Meyer)

Cited by 44 publications

References 4 publications

Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)

Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)

Analysis of Genetic Polymorphism of Korean Ginseng Cultivars and Foreign Accessions using SSR Markers

Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers

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