Development and characterization of microsatellite loci from <i>Olea europaea</i>

Gil, Fabíola Sabino; Busconi, Matteo; Machado, Artur da Câmara; Fogher, Corrado

doi:10.1111/j.1471-8286.2006.01513.x

Cited by 34 publications

(9 citation statements)

References 11 publications

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“…The majority of SSRs currently identified for olive genotyping carry di-nucleotide repeats (Carriero et al 2002;Cipriani et al 2002;de la Rosa et al 2002;Diaz et al 2006;Rallo et al 2000;Sabino Gil et al 2006;Sefc et al 2000), mostly AG/CT repeats. In order to establish a common set of markers able to produce reliable data for olive cultivar discrimination, Baldoni et al (2009) provided a consensus list of di-nucleotide SSR markers.…”

Section: Communicated By G G Vendraminmentioning

confidence: 99%

Development, evaluation, and validation of new EST-SSR markers in olive (Olea europaea L.)

Mariotti

Cultrera

Mousavi

et al. 2016

Tree Genetics & Genomes

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Rapid and effective genotyping is an important goal to discriminate among the numerous olive cultivars and their wild related forms. The largely used di-nucleotide simple sequence repeat (SSR) markers show a high level of polymorphism and have strongly contributed to solve many inconsistencies in varietal identity, but many problems related to difficult discrimination of neighboring alleles and low comparability of data among different labs severely reduce their applicability for large-scale screening. The availability of numerous transcriptome libraries, which were developed from different tissues of several olive varieties, has allowed their intensive screening to search for polynucleotide microsatellite regions with long core repeats, potentially polymorphic among varieties. An accurate screening of all these polymorphisms has allowed to select a set of 25 trinucleotide and one tetranucleotide SSRs, showing a good level of discrimination power with a high allele pattern resolution and repeatability. They were preliminarily tested on a group of cultivated varieties then validated on a wider group of cultivated and wild plants, and related species and subspecies, demonstrating a good transferability within the entire Olea taxon. Furthermore, an in silico functional prediction has allowed to assign each transcribed sequence to their gene functions and biological process categories, highlighting their potential application of these new EST-SSRs as functional markers.

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Section: Communicated By G G Vendraminmentioning

confidence: 99%

Development, evaluation, and validation of new EST-SSR markers in olive (Olea europaea L.)

Mariotti

Cultrera

Mousavi

et al. 2016

Tree Genetics & Genomes

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“…Microsatellites are highly popular genetic markers because of their codominant inheritance, high abundance, enormous extent of allelic diversity, and the ease of assessing simple sequence repeat (SSR) size variation by PCR with pairs of flanking primers (Agarwal et al, 2008;Muzzalupo et al, 2006Rekik et al, 2008). Recently, many efforts have been made to develop good and reliable molecular tools such as microsatellite markers that could shed light on olive tree genetics (Carriero et al, 2002;Cipriani et al, 2002;De la Rosa et al, 2002;Diaz et al, 2006;Rallo et al, 2000;Sabino Gil et al, 2006;Sefc et al, 2000). They have been used for cultivar identification for elucidating genetic relationships between several olive cultivars (Muzzalupo et al, 2006.…”

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confidence: 99%

Evaluation of Olives Cultivated in Southern Italy by Simple Sequence Repeat Markers

Muzzalupo¹,

Stefanizzi²,

Perri³

2009

horts

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Olive (Olea europaea L.) is a species of great economic importance in the Mediterranean basin. Italy is very important for the olive industry; in fact, olive's genetic patrimony is very rich and characterized by an abundance of cultivars. At present, the majority of ancient landraces are vegetatively propagated by farm. It is likely that the number of cultivars is underestimated because of inadequate information on minor local cultivars that are widespread in different olive-growing areas. The existence of many cultivars reinforces the need for a reliable identification method. It is important to improve the ex situ plant germplasm collection and fairly to characterize all cultivars for future breeding programs. In the present report, we used 11 loci microsatellites to characterize 211 olive cultivars of an olive collection cultivated in six regions of southern Italy. These regions represent the major area for olive cultivation in Italy and have a strategic geographical location in the Mediterranean basin. The dendrogram obtained, using the unweighted pair group method with arithmetic mean clustering algorithm, depicts the pattern of relationships between the studied cultivars. There is a clear structuring of the variability relative to the geographic origin of olive cultivars. This work, for the very high number of the Italian olive cultivars analyzed, highlights the degree and distribution of genetic diversity of this species for better exploitation of olive resources and for the design of plant breeding programs. Besides, the use of molecular markers, like simple sequence repeats, is imperative to build a database for cultivar analysis, for traceability of processed food, and for appropriate management of olive germplasm collections.

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“…Many authors have reported on the development of SSRs in olive and several different sets of markers are actually available for DNA analysis (Sabino Gil et al, 2006).…”

Section: Ssrsmentioning

confidence: 99%

Olive Genomics

Fogher

Busconi

Sebastiani

et al. 2010

Olives and Olive Oil in Health and Disease Prevention

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Development and characterization of microsatellite loci from Olea europaea

Abstract: This study reports 19 simple sequence repeat loci developed from a genomic library of the olive tree ( Olea europaea L.), of which 12 revealed to be polymorphic and informative, ranging from two to 14 alleles.

Cited by 34 publications

References 11 publications

Development, evaluation, and validation of new EST-SSR markers in olive (Olea europaea L.)

Development, evaluation, and validation of new EST-SSR markers in olive (Olea europaea L.)

Evaluation of Olives Cultivated in Southern Italy by Simple Sequence Repeat Markers

Olive Genomics

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