Development and characterization of microsatellite markers in the sexual-apomictic complex Taraxacum officinale (dandelion)

Falque, Matthieu; Keurentjes, Joost J. B.; Bakx-Schotman, J.M.T.; Dijk, Peter J. van

doi:10.1007/s001220050897

Cited by 49 publications

(51 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, for a more informative interpretation of allelic configurations, the MAC-PR approach, that is, quantitative comparisons of allele peak ratios, proved very useful. Corresponding attempts have been reported in triploid and apomictic Taraxacum genotypes, but it was usually not possible to infer the exact genotype from the relative intensity of bands in those genotypes that showed only two bands (Falque et al, 1998). Carefully optimised experimental conditions are of course a prerequisite for successful quantification of allele copy number.…”

Section: Discussionmentioning

confidence: 99%

Microsatellite DNA marker inheritance indicates preferential pairing between two highly homologous genomes in polyploid and hemisexual dog-roses, Rosa L. Sect. Caninae DC.

Nybom

Esselink²,

Werlemark

et al. 2003

Heredity

View full text Add to dashboard Cite

According to previous cytological evidence, the hemisexual dog-rose species, Rosa sect. Caninae, transmit only seven chromosomes (derived from seven bivalents) through their pollen grains, whereas egg cells contain 21, 28 or 35 chromosomes (derived from seven bivalents and 14, 21 or 28 univalents) depending on ploidy level. Two sets of reciprocal pairwise interspecific crosses involving the pentaploid species pair R. dumalis and R. rubiginosa, and the pentaploid/tetraploid species pair R. sherardii and R. villosa, were analysed for 13 and 12 microsatellite DNA loci, respectively. Single loci were represented by a maximum of three simultaneously occurring alleles in R. villosa, and four alleles in the other three parental plants. In the experimentally derived offspring, the theoretical maximum of five alleles was found for only one locus in the pentaploid progenies. Microsatellite DNA allele composition was identical with that of the maternal parent in 10 offspring plants, which were probably derived through apomixis. Almost all microsatellite DNA alleles were shared with the maternal parent also in the remaining offspring, but 1-4 alleles shared only with the paternal parent, indicating sexual seed formation. Analysis of quantitative peak differences allowed a tentative estimation of allelic configuration in the individual plants, and suggested that bivalent formation preferentially takes place between chromosomes that consistently share the same microsatellite alleles and therefore appear to be highly homologous. Moreover, alleles that were shared between the species in each cross combination comparatively often appear to reside on the bivalent-forming chromosomes, whereas species-specific alleles instead occur comparatively often on the univalent-forming chromosomes and are therefore inherited through the maternal parent only. Recombination then takes place between very similar genomes also in interspecific crosses, resulting in a reproduction system that is essentially a mixture between apomixis and selfing.

show abstract

Section: Discussionmentioning

confidence: 99%

Microsatellite DNA marker inheritance indicates preferential pairing between two highly homologous genomes in polyploid and hemisexual dog-roses, Rosa L. Sect. Caninae DC.

Nybom

Esselink²,

Werlemark

et al. 2003

Heredity

View full text Add to dashboard Cite

show abstract

“…DNA was isolated by the NaOH extraction method of Wang et al (1993). PCR conditions for the four microsatellite loci MSTA 61, MSTA 64, MSTA 72 and MSTA 78 were as described by Falque et al (1998).…”

Section: Methodsmentioning

confidence: 99%

“…After germination, all seedlings were cytotyped by flow cytometry and genotyped for the four microsatellite loci MSTA 53, MSTA 61, MSTA 72 and MSTA 78 (Falque et al, 1998). The PCR products were analysed on an ALF Express II sequencer (Amersham Pharmacia Biotech, Uppsala, Sweden).…”

Section: Methodsmentioning

confidence: 99%

The role of tetraploids in the sexual–asexual cycle in dandelions (Taraxacum)

2004

View full text Add to dashboard Cite

Apomictic plants often produce pollen that can function in crosses with related sexuals. Moreover, facultative apomicts can produce some sexual offspring. In dandelions, Taraxacum, a sexual-asexual cycle between diploid sexuals and triploid apomicts, has been described, based on experimental crosses and population genetic studies. Little is known about the actual hybridization processes in nature. We therefore studied the sexual-asexual cycle in a mixed dandelion population in the Netherlands. In this population, the frequencies of sexual diploids and triploids were 0.31 and 0.68, respectively. In addition, less than 1% tetraploids were detected. Diploids were strict sexuals, triploids were obligate apomicts, but tetraploids were most often only partly apomictic, lacking certain elements of apomixis. Tetraploid seed fertility in the field was significantly lower than that of apomictic triploids. Field-pollinated sexual diploids produced on average less than 2% polyploid offspring, implying that the effect of hybridization in the 2x-3x cycle in Taraxacum will be low. Until now, 2x-3x crosses were assumed to be the main pathway of new formation of triploid apomicts in the sexual-asexual cycle in Taraxacum. However, tetraploid pollen donors produced 28 times more triploid offspring in experimental crosses with diploid sexuals than triploid pollen donors. Rare tetraploids may therefore act as an important bridge in the formation of new triploid apomicts.

show abstract

“…Four microsatellite loci were used for genotyping the plants: msta64, msta72, msta78 and msta85 (Falque et al, 1998). Microsatellite amplification products were run on polyacrylamide on a Li-Cor GeneReadir 4200 sequencer.…”

Section: Scoring Of Male Sterilitymentioning

confidence: 99%

“…It is known that pollination of a diploid sexual motherplant with pollen from a triploid asexual can result in breakdown of the self-incompatibility system in the sexual (Morita et al, 1990); it is, therefore, important to check for selfing of the mother plants. We did this through genotyping the parents and all the offspring that survived to maturity at three microsatellite loci: msta53, msta72 and msta78 (Falque et al, 1998). All plants of the progeny that came out as hybrids were subsequently checked for male sterility when they flowered.…”

Section: Crossesmentioning

confidence: 99%

Male sterility in triploid dandelions: asexual females vs asexual hermaphrodites

Meirmans

Nijs²,

Tienderen³

2005

Heredity

View full text Add to dashboard Cite

Male reproductive output, pollen in plants and sperm in animals has been shown to constitute a substantial cost for many organisms. In parthenogenetic hermaphrodites, selection is therefore expected to reduce the allocation of resources to male reproductive output. However, sustained production of pollen or sperm has been observed in numerous asexual hermaphrodites. We studied the widespread production of pollen by triploid asexual dandelions, Taraxacum sect. Ruderalia, comparing rare male sterile individuals with pollen producing asexuals. We found that individuals can show plasticity in the production of pollen, but that it is nevertheless possible to distinguish between (facultatively) male sterile asexuals and male fertile asexuals. Based on evidence from genetic markers and crosses, we conclude that the male sterility in asexual dandelions is caused by nuclear genes, in contrast to the cytoplasmically inherited male sterility previously found in sexual dandelions. Male sterile lineages did not produce more seeds per flower head, heavier seeds or seeds that were more viable. However, male sterile plants did produce more seed heads and hence more seeds than pollen producing ones, indicating that they were able to reallocate resources toward seed production. Considering the difference in seed production, it remains puzzling that not more asexual dandelions are male sterile.

show abstract

Development and characterization of microsatellite markers in the sexual-apomictic complex Taraxacum officinale (dandelion)

Cited by 49 publications

References 26 publications

Microsatellite DNA marker inheritance indicates preferential pairing between two highly homologous genomes in polyploid and hemisexual dog-roses, Rosa L. Sect. Caninae DC.

Microsatellite DNA marker inheritance indicates preferential pairing between two highly homologous genomes in polyploid and hemisexual dog-roses, Rosa L. Sect. Caninae DC.

The role of tetraploids in the sexual–asexual cycle in dandelions (Taraxacum)

Male sterility in triploid dandelions: asexual females vs asexual hermaphrodites

Contact Info

Product

Resources

About