Development and characterization of microsatellite primers in the federally endangered <i>Astragalus bibullatus</i> (Fabaceae)

Morris, Ashley B.; Scalf, Cassandra; Burleyson, Austin; Johnson, La Tonya; Trostel, Kevin

doi:10.3732/apps.1500126

Cited by 11 publications

(9 citation statements)

References 14 publications

(14 reference statements)

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“…Molecular data were confirmed by morphometric analyses, and both sets of results supported segregation of the pink-flowered form of A. agnicidus as a new taxon (Meinke et al, 2013). Morris et al (2016) found a suite of novel nuclear microsatellite loci in the federally endangered species A. bibullatus using next-generation sequencing. They tested 12 loci, which were selected based on clarity of amplification and ease of scoring from the initial samples for A. bibullatus and cross-amplification in A. crassicarpus var.…”

Section: Genetic Diversitymentioning

confidence: 99%

Reproductive biology, ecological life history/demography and genetic diversity of the megagenus Astragalus (Fabaceae, Papilionoideae)

et al. 2021

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Astragalus is the largest genus of seed plants; however, information on its reproductive biology and life history is widely scattered in the literature. About 96% of the species are perennials and 4% annuals; many are rare endemics. Astragalus species are obligate or facultative outcrossers, and inbreeding depression is higher in self-compatible (SC) than in self-incompatible (SI) species. The most frequent pollinators are Bombus, Osmia and Anthophora. On average, 48% of ovules produce seeds, and 40% of flowers produce fruits. Seed/ovule and fruit/flower ratios are lower in SC than in SI species. Predispersal insect seed predation ranges from 0 to 93%, and major predators are Acanthoscelides, Bruchophagus and Tychius. Seeds have physical dormancy and typically form a seed bank. C 3 is the only photosynthetic pathway in the genus. Population growth may be cyclic or noncyclic, and genetic diversity of a taxon may or may not be related to size of geographic range.

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Section: Genetic Diversitymentioning

confidence: 99%

Reproductive biology, ecological life history/demography and genetic diversity of the megagenus Astragalus (Fabaceae, Papilionoideae)

et al. 2021

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“…Genomic sequencing has revolutionised discovery of such markers (Zalapa et al 2012), whether speciesspecific (Abdelkrim et al 2009) or for use across a broader taxonomic group (Hodel et al 2016), including for several legumes (e.g. Borges et al 2015;Morris et al 2016). For example, microsatellite markers developed from the Lotus japonicus (Regel) K.Larsen genome (Sato et al 2008) were used through cross-species amplification in Lotus sessilifolius DC., a species endemic to Macaronesia (Yang et al 2018).…”

Section: Conservation Biology and Geneticsmentioning

confidence: 99%

Advances in legume research in the genomics era

Egan

Vatanparast

2019

Aust. Syst. Bot.

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Next-generation sequencing (NGS) technologies and applications have enabled numerous critical advances in legume biology, from marker discovery to whole-genome sequencing, and will provide many new avenues for legume research in the future. The past 6 years in particular have seen revolutionary advances in legume science because of the use of high-throughput sequencing, including the development of numerous types of markers and data useful for evolutionary studies above and below the species level that have enabled resolution of relationships that were previously unattainable. Such resolution, in turn, affords opportunities for hypothesis testing and inference to improve our understanding of legume biodiversity and the patterns and processes that have created one of the most diverse plant families on earth. In addition, the genomics era has seen significant advances in our understanding of the ecology of legumes, including their role as nitrogen fixers in global ecosystems. The accumulation of genetic and genomic data in the form of sequenced genomes and gene-expression profiles made possible through NGS platforms has also vastly affected plant-breeding and conservation efforts. Here, we summarise the knowledge gains enabled by NGS methods in legume biology from the perspectives of evolution, ecology, and development of genetic and genomic resources.

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“…The abundance of these repetitive regions is highly variable among taxa, which is useful for distinguishing individuals (OLIVEIRA et al, 2006). However, the identification of these regions demands on knowledge of genome sequences (MORRIS, 2016).…”

Section: Introductionmentioning

confidence: 99%

In silico analysis of Pinus L. chloroplast DNA to microsatellites regions

Moro¹,

Pereira²,

Filho³

et al. 2019

Sci. For.

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The Pinus genus covers a wide variety of widely cultivated species due to adaptability, high growth and wood quality. Molecular markers have been used for many genetic analyses, and among them, the microsatellite markers (SSR) have several applications and can be found in chloroplast genome (cpDNA) as well as in nuclear genomes (ntDNA). The chloroplast microsatellites markers (cpSSR) can be used for gene flow analysis, identification of hybrids,clones, paternity tests, genetic diversity studies, phylogenetic analysis, among others. The work aimed to characterize the cpSSRs of Pinus species with cpDNA sequenced and deposited in NCBI (National Center for Biotechnology Information). In the twenty species of Pinus spp. studied, 1.542 cpSSRs were identified, with 86,45% of mononucleotide type, and the less frequent the penta-(1.10%) and hexanucleotide (1.04%) types. Predominated cpSSRs in non-coding regions (intergenic). The results indicate presence of a wide range of cpSSR for Pinus spp., which can subsidize breeding programs of interesting species.

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Development and characterization of microsatellite primers in the federally endangered Astragalus bibullatus (Fabaceae)

Cited by 11 publications

References 14 publications

Reproductive biology, ecological life history/demography and genetic diversity of the megagenus Astragalus (Fabaceae, Papilionoideae)

Reproductive biology, ecological life history/demography and genetic diversity of the megagenus Astragalus (Fabaceae, Papilionoideae)

Advances in legume research in the genomics era

In silico analysis of Pinus L. chloroplast DNA to microsatellites regions

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