Development and characterization of microsatellite loci for the pseudometallophyte <i>Commelina communis</i> (Commelinaceae)

Li, Jiaokun; Song, Yanqiu; Xu, Hui; Zhu, Jianyu; Tang, Lulu

doi:10.3732/apps.1400098

Cited by 9 publications

(7 citation statements)

References 11 publications

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“…Polymorphism was assayed on each DNA sample with 12 microsatellite markers developed in our previous study (Supplementary Table S1 ; Li et al, 2015b ). The amplification reaction was carried out in 20 μL reaction mixture, containing 30–50 ng genomic DNA, 7.5 μL of 2× Ta q PCR MasterMix (Tiangen, Beijing, China), 0.6 μM of each primer.…”

Section: Methodsmentioning

confidence: 99%

Geography Plays a More Important Role than Soil Composition on Structuring Genetic Variation of Pseudometallophyte Commelina communis

Song

et al. 2016

Front. Plant Sci.

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Pseudometallophytes are excellent models to study microevolution and local adaptation to soil pollution, as they can grow both on metalliferous and contrasting non-metalliferous soils. Although, there has been accumulating evidence for the effects of edaphic conditions and geographical isolation on the genetic structure of pesudometallophytes, it is still a difficult problem in evolutionary biology to assess their relative importance. In this study, we investigated the spatial patterns of genetic variability, population differentiation and genetic groups in pseudometallophyte Commelina communis with 12 microsatellite loci. Eight metallicolous and six non-metallicolous populations of C. communis were sampled from cupriferous sites and surrounding non-contaminated areas in China. Neither significant reduction in genetic diversity nor apparent founder and bottleneck effects were observed in metallicolous populations of C. communis. Based on Bayesian and Neighbor-Joining clustering analyses and a principal coordinates analysis, all sampled populations were found to be mainly separated into three genetic groups, corresponding well to their geographical locations rather than edaphic origins. Moreover, a significant and strong correlation between population genetic divergence and geographical distance were detected by Mantel test (r = 0.33; P < 0.05) and multiple matrix regression with randomization (MMRR; βD = 0.57, P < 0.01). However, the effect of copper concentration on genetic patterns of C. communis was not significant (MMRR; βE = -0.17, P = 0.12). Our study clearly demonstrated that the extreme edaphic conditions in metalliferous areas had limited effects on the genetic variability in C. communis. Geographic distance played a more important role in affecting the genetic structure of C. communis than soil composition did. In C. communis, the geographically disjunctive populations on metalliferous soils had multiple origins and evolved independently from nearby non-metallicolous populations.

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Section: Methodsmentioning

confidence: 99%

Geography Plays a More Important Role than Soil Composition on Structuring Genetic Variation of Pseudometallophyte Commelina communis

Song

et al. 2016

Front. Plant Sci.

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“…C. communis has a mixed mating system (Katsuhara & Ushimaru, 2019 ; Morita & Nigorikawa, 1999 ), and our results revealed relatively high inbreeding coefficients ( F IS ) and low H O in both rural and urban populations (Figure 2c , Figure S4a ). H O values of some SSR loci were also lower than H E in C. communis (Li et al., 2015 ). These findings suggest that the lack of genetic diversity differences among habitat types is attributable to a high population selfing ratio across the urban–rural gradient.…”

Section: Discussionmentioning

confidence: 99%

Habitat diversification associated with urban development has a little effect on genetic structure in the annual native plant Commelina communis in an East Asian megacity

Taichi,

Nakahama,

Ohmido

et al. 2024

Ecology and Evolution

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Urban development greatly alters the natural and semi‐natural habitats of native plants. Urbanisation results in a range of diverse habitats including remnant agricultural lands, urban parks, and roadside habitats. This habitat diversity often promotes trait divergence within urban areas. However, the mechanisms by which diverse urban habitats influence the population genetic structure of individual plant species remain poorly understood. We investigated the effects of urbanisation on genetic diversity and structure within 24 Commelina communis populations across diverse habitat types (rural agricultural land, urban agricultural land, urban park land, and urban roadsides) within the Kyoto–Osaka–Kobe megacity in Japan. We conducted multiplexed inter‐simple sequence repeat genotyping to compare genetic diversity among populations in different habitats. We also examined the correlation between Nei's genetic distance and geographic and environmental distances and performed principal coordinate analysis (PCoA) to evaluate genetic differentiation among urban habitats. There were no significant differences in genetic diversity indices between urban and rural populations and among urban habitat types. Although we detected no isolation‐by‐distance structure in population pairs of the same habitat type and in those of different habitats, the difference in surrounding landscape facilitated genetic differentiation not only between urban and rural habitats but also between different urban habitats. PCoA revealed no clear genetic differentiation among rural and urban habitat populations. Our findings indicate that the establishment of diverse habitat types through urbanisation has no and little impact on genetic diversity and structure, respectively, in C. communis, likely due to its high selfing rate and ability to adapt to urban conditions.

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“…Free radicals can decrease the concentration of intracellular ATP, calcium leakage from the mitochondria, osmotic imbalance and lipid peroxidation by increasing permeability and cell damage [28]. At the same time, natural antioxidants of plant origin can strengthen endogenous antioxidants for defence against ROS and restore the optimal balance by neutralizing them [26].…”

Section: Antioxidant Assay Dpph Free Radical Scavenging Assaymentioning

confidence: 99%

Protective and Antioxidant Capacity of Date Palm Seeds (Phoenix Dactylifera L.) on Hepatotoxicity in Rats

Ghania¹

2023

FARMACIA

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In recent years, the population has preferred using phytopharmaceutical products to reduce the complications involving xenobiotics in various pathologies. This study aimed to evaluate the antitoxic activity of three varieties of Phoenix dactyliferaL. Seeds in vivo, namely Deglet Nour, Mech-Degla, and Ghars. Doses of 200 mg/kg bw/day of their extracts were administered to male Wistar albino rats intoxicated using 50% of carbon tetrachloride (CCl4). The results were compared to silymarin. After 15 days, rats were sacrificed, blood samples were collected for the serum biochemical parameters analysis, and the liver was removed for the oxidative stress enzymes analysis and histopathological study. Extraction results from Deglet Nour, Mech-Deglea, and Ghars yielded different yields of 3.16%, 4.55% and 2.07%, respectively. However, the Deglet Nour variety had the highest antioxidant power with an IC50 of 0.231 compared to the other extracts. The polyphenolic contents range between 189.42 and 246.57 mg GAE/mL. Generally, the studied varieties showed exciting results with their regulatory capacity against the serum enzymes rates of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl transpeptidase (GGT) that were disturbed by the CCl4 treatment in addition to the biochemical parameters such as; total protein, glucose levels, cholesterol, and triglycerides. The examined enzymes for oxidative stress were malondialdehyde (MDA) and glutathione (GSH). Several unusual activities were seen in the rats treated with the different seed extracts as a response to the intoxication generated by the administered xenobiotic. The active compounds in the three varieties of date palm seeds outperformed silymarin in terms of regulating power in some metrics. Histological changes accompanied the metabolic response of treated rats to the various extracts. A remarkable inflammation in the liver tissue was observed in the control group. At the same time, the raw extracts of date seeds have proved their capacity to minimize rashes, and the serum biochemical results were similar to the conducted histological study. RezumatUtilizarea produselor fitofarmaceutice a crescut în ultimii ani, populația alegând această opțiune terapeutică în defavoarea xenobioticelor și a reacțiilor adverse pe care le implică administrarea acestora în diverse patologii. Acest studiu a avut ca scop evaluarea activității antitoxice a trei soiuri de Phoenix dactylifera L. Seeds in vivo, și anume Deglet Nour, Mech-Degla și Ghars. Au fost administrate doze de 200 mg/kg corp/zi din cele 3 extracte la șobolani Wistar Albino masculi intoxicați în prealabil cu tetraclorură de carbon 50% (CCl4), folosindu-se ca și control silimarina. După 15 zile, șobolanii au fost sacrificați, li s-au recoltat probe de sânge pentru analiza parametrilor biochimici serici, iar ficatul a fost prelevat pentru analiza enzimelor de stres oxidativ și pentru studiul histopatologic. Randamentele de extracție din Deglet Nour, Mech-Degla și Ghars a...

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Development and characterization of microsatellite loci for the pseudometallophyte Commelina communis (Commelinaceae)

Cited by 9 publications

References 11 publications

Geography Plays a More Important Role than Soil Composition on Structuring Genetic Variation of Pseudometallophyte Commelina communis

Geography Plays a More Important Role than Soil Composition on Structuring Genetic Variation of Pseudometallophyte Commelina communis

Habitat diversification associated with urban development has a little effect on genetic structure in the annual native plant Commelina communis in an East Asian megacity

Protective and Antioxidant Capacity of Date Palm Seeds (Phoenix Dactylifera L.) on Hepatotoxicity in Rats

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