Development and characterization of human iPSC line NCCSi004-A from umbilical cord blood (UCB) derived CD34+cells obtained from donor belonging to Indian ethnic population

Fernandes, Sophia; Tembe, Shruti; Singh, Sanjay Kumar; Vardhan, Shakti; Nair, Velu; Kale, Vaijayanti; Limaye, Lalita

doi:10.1016/j.scr.2019.101392

Cited by 3 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are promising in showing that direct application of EiPSCsderived cardiomyocytes is possible. The local environment and conditions under which the EiPSCs were directly Bharathan et al 36 , Weltner et al 37 , Yu et al 40 , Wong et al 77 , Bang et al 78 , Hey et al 79 , Jaffer et al 80 , Trevisan et al 81 , Fidan et al 82 [165][166][167] Amniotic fluid cells injected allowed for their direct use and differentiation according to clinical need. A diagram of the potential application for an EiPSCs-engineered cardiac cell sheet is shown in Fig.…”

Section: In Vivo Animal Model Applications Cardiogenic Regenerationmentioning

confidence: 99%

Episomal Induced Pluripotent Stem Cells: Functional and Potential Therapeutic Applications

Wang

Loh

2019

Cell Transplant

View full text Add to dashboard Cite

The term episomal induced pluripotent stem cells (EiPSCs) refers to somatic cells that are reprogrammed into induced pluripotent stem cells (iPSCs) using non-integrative episomal vector methods. This reprogramming process has a better safety profile compared with integrative methods using viruses. There is a current trend toward using episomal plasmid reprogramming to generate iPSCs because of the improved safety profile. Clinical reports of potential human cell sources that have been successfully reprogrammed into EiPSCs are increasing, but no review or summary has been published. The functional applications of EiPSCs and their potential uses in various conditions have been described, and these may be applicable to clinical scenarios. This review summarizes the current direction of EiPSC research and the properties of these cells with the aim of explaining their potential role in clinical applications and functional restoration.

show abstract

Section: In Vivo Animal Model Applications Cardiogenic Regenerationmentioning

confidence: 99%

Episomal Induced Pluripotent Stem Cells: Functional and Potential Therapeutic Applications

Wang

Loh

2019

Cell Transplant

View full text Add to dashboard Cite

show abstract

“…P53 promotes the expression of MDM2 [33,34] and miR-200 represses the translation of OCT4 [35]. MDM2 inhibits the expression of P53 [34] and is inhibited by miR-145 [31].…”

Section: Model Constructionmentioning

confidence: 99%

A physical mechanism of heterogeneity in stem cell, cancer and cancer stem cell

Yu,

Liu,

Chen

et al. 2019

Preprint

View full text Add to dashboard Cite

Heterogeneity is ubiquitous in stem cells (SC), cancer cells (CS), and cancer stem cells (CSC). SC and CSC heterogeneity is manifested as diverse sub-populations with self-renewing and unique regeneration capacity. Moreover, the CSC progeny possesses multiple plasticity and cancerous characteristics. Many studies have demonstrated that cancer heterogeneity is one of the greatest obstacle for therapy. This leads to the incomplete anti-cancer therapies and transitory efficacy. Furthermore, numerous micro-metastasis leads to the wide spread of the tumor cells across the body which is the beginning of metastasis. The epigenetic processes (DNA methylation or histone remodification etc.) can provide a source for certain heterogeneity. In this study, we develop a mathematical model to quantify the heterogeneity of SC, CSC and cancer taking both genetic and epigenetic effects into consideration. We uncovered the roles and physical mechanisms of heterogeneity from the three aspects (SC, CSC and cancer). In the adiabatic regime (relatively fast regulatory binding and effective coupling among genes), seven native states (SC, CSC, Cancer, Premalignant, Normal, Lesion and Hyperplasia) emerge. In non-adiabatic regime (relatively slow regulatory binding and effective weak coupling among genes), multiple meta-stable SC, CS, CSC and differentiated states emerged which can explain the origin of heterogeneity. In other words, the slow regulatory binding mimicking the epigenetics can give rise to heterogeneity. Elucidating the origin of heterogeneity and dynamical interrelationship between intra-tumoral cells has clear clinical significance in helping to understand the cellular basis of treatment response, therapeutic resistance, and tumor relapse.

show abstract

A physical mechanism of heterogeneity and micro-metastasis in stem cells, cancer cells, and cancer stem cells

Liu

Wang

2022

The Journal of Chemical Physics

View full text Add to dashboard Cite

Heterogeneity is ubiquitous in stem cells (SC), cancer cells (CS), and cancer SC (CSC). This heterogeneity manifests in the form of diverse sub-populations of SC, CS, and CSC with self-renewal and unique regeneration capacity. Moreover, the progeny of CSC possess multiple plasticity and cancerous characteristics. Many studies have demonstrated that cancer heterogeneity is among the greatest obstacles to successful anti-cancer therapy. This leads to incomplete therapy and transitory efficacy. Furthermore, numerous micro-metastases lead to the spread of tumor cells widely across the body; this is the beginning of metastasis. Epigenetic processes (DNA methylation and histone remodification) represent sources of heterogeneity. In this study, we develop a mathematical model to quantify the heterogeneity of SC, CS, and CSC, taking into consideration both genetic and epigenetic effects. We reveal the roles and physical mechanisms of heterogeneity in SC, CSC, and cancer cells. Under the adiabatic regime (relatively fast regulatory binding and effective coupling among genes), seven native states (SC, CSC, cancer, premalignant, normal, lesion, and hyperplasia) emerge. Under the non-adiabatic regime (relatively slow regulatory binding and effective weak coupling among genes), SC, CS, CSC, and differentiated states emerge and become diffusive, partially explaining the origin of heterogeneity. In other words, slow regulatory binding that mimics epigenetic effects can give rise to heterogeneity. Moreover, we calculated the entropy production rate and Fano factor, which can be used to quantify the thermodynamic cost and the degrees of the variations or the fluctuations as the parameter ω (representing the speed of regulatory binding/unbinding relative to the synthesis/degradation) changes. Elucidating the origins of heterogeneity and the dynamical relationships between intra-tumoral cells has a clear clinical significance and will improve the understanding of the cellular basis of treatment response, therapeutic resistance, and cancer metastasis.

show abstract

Development and characterization of human iPSC line NCCSi004-A from umbilical cord blood (UCB) derived CD34+cells obtained from donor belonging to Indian ethnic population

Cited by 3 publications

References 2 publications

Episomal Induced Pluripotent Stem Cells: Functional and Potential Therapeutic Applications

Episomal Induced Pluripotent Stem Cells: Functional and Potential Therapeutic Applications

A physical mechanism of heterogeneity in stem cell, cancer and cancer stem cell

A physical mechanism of heterogeneity and micro-metastasis in stem cells, cancer cells, and cancer stem cells

Contact Info

Product

Resources

About