Development and characterization of DNA microsatellite primers for buriti (Mauritia flexuosa L.f.)

Abstract

ABSTRACT. Mauritia flexuosa L. (Arecaceae) is a palm tree species known as buriti that occurs in the Cerrado biome. It is characteristic of the vereda, a typical ecosystem of central Brazil. In this phytophysiognomy, M. flexuosa and other groups of arboreal-herbaceous species develop in open fields with very humid soils. M. flexuosa can be found in forest borders and is a palm tree with a wide distribution in South America (Brazil, Colombia, Venezuela, French Guyana Ecuador, Peru, and Bolivia). The main objectives of this study were to develop simple sequence repeat marker-enriched libraries and to characterize these loci in buriti palm to facilitate future population studies. A total of 40 sequences derived from the microsatellite-enriched libraries were selected for primer design. The optimization results showed that 9 primer pairs could successfully amplify polymorphic target fragments of the expected sizes. The data also show that the described primers Development of microsatellite primers for buriti can be used in population genetic studies in M. flexuosa to obtain information that will inform conservation and management strategies.

Rivers shape population genetic structure in Mauritia flexuosa (Arecaceae)

“…DNA was extracted following the manufacturer's protocol of the Wizard Genomic DNA Purification kit (Promega, Madison, WI, USA). We selected 10 microsatellites previously designed for M. flexuosa (Federman, Hyseni, Clement, & Caccone, 2012; Menezes et al., 2012; Table S1) based on consistency of amplification. PCR conditions for all primers in individual reactions were 94°C for 5 min; 35 cycles of 94°C for 1 min, 62°C for 1 min, and 72°C for 1 min; then 72°C for 2 min.…”

Mauritia flexuosa palm swamp communities: natural or human-made? A palynological study of the Gran Sabana region (northern South America) within a neotropical context

“…The better known cultivation experiences are in Perú, where several plantations are under exploitation and a research institute is conducting experiments on seed selection and germination (Delgado et al, 2007), and Venezuela, where some artificial plantations exist (Delascio, 1999). Some preliminary research on genetic features and germplasm viability has been conducted (Gonzales et al, 2006;Gomes et al, 2011;Menezes et al, 2012) but the potential application of these studies to cultivation practices is still in its infancy.…”

Genetic structure analysis of Mauritia flexuosa natural population from the Lençóis Maranhenses region using microsatellite markers

“…Current knowledge regarding the genetic structure of the natural populations of M. flexuosa distributed in different regions of Brazil is based on analyses of certain molecular markers: AFLP (Gomes et al, 2011), ISSR (Rossi et al, 2014) and SSR (Federman et al, 2012;Menezes et al, 2012). However, studies of this species are still scarce, with no evaluation of the genetic structure regarding the distribution of natural buriti populations from Lençóis Maranhenses.…”

“…The relevance of this study is due to the marked environmental degradation of this region caused by advances in road infrastructure and the expansion of agribusiness and urban borders. Specific SSR markers for this species were developed and applied to populations in other areas (Federman et al, 2012;Menezes et al, 2012; groups were allocated only the smaller populations of M. flexuosa (Figure 4). Thus, the populations of this species were easily separated using SSR markers, indicating genetic differentiation and correlations between the natural populations of this plant.…”