Development and characterization of cross-linked laccase aggregates (Lac-CLEAs) from Trametes versicolor IBL-04 as ecofriendly biocatalyst for degradation of dye-based environmental pollutants

Bilal, Muhammad; Noreen, Sadia; Asgher, Muhammad; Parveen, Sofia

doi:10.1016/j.eti.2021.101364

Cited by 25 publications

(11 citation statements)

References 55 publications

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“…It is interesting to highlight the higher immobilization recovery obtained in this work compared with previous works on CLEAs with other enzymes, in which the best immobilization rate did not go up from 30% to 70% for PEG, ammonium sulfate, n-propanol, acetone, and tert-butanol as precipitant agents, whereas in the present study, the results up to 80% were obtained for all the enzymes studied ( Bilal et al, 2021 ; Xu et al, 2020 ; Perwez et al, 2021 ).…”

Section: Resultscontrasting

confidence: 72%

“…Analyzing results deeper, the immobilized percentage for all the conditions was slightly lower than the one obtained with the previous enzymes, which could be explained because it is more difficult to immobilize more enzymes compared to one due to the complex interaction that could appear between all of them. Although, the percentages achieved could be considered as high ones compared to previous works on CLEAs with other enzymes ( Bilal et al, 2021 ; Xu et al, 2020 ; Perwez et al, 2021 ).…”

Section: Resultsmentioning

confidence: 69%

“…There were clear differences between acetone at 70% and 90%, which were similar in terms of immobilization percentage. Acetone at 70% lost 50% of its initial activity at 30 min, whereas acetone at 90% achieve this level at 2 h. Nevertheless, both of them only retains 20% of its initial activity at 4 h. In addition, it could be seen big differences between PEG at different rates, showing better stability 60% compared to 50% ( Sheldon, 2006 ; Bilal et al, 2021 ).…”

Section: Resultsmentioning

confidence: 95%

“…In this work, the effect of the cross-linker was not studied, and GA was used for CLEA production, as it was previously optimized in other studies. On the contrary, the action of the precipitant agent was deeply studied due to the potential interaction of the different enzymes with them due to their different structures ( Velasco-Lozano, 2020 ; Hero et al, 2020 ; Xu et al, 2020 ; Bilal et al, 2021 ; Guajardo et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Cross-Linked Enzyme Aggregates and Their Application in Enzymatic Pretreatment of Microalgae: Comparison Between CLEAs and Combi-CLEAs

Blanco-Llamero

García-García

Señoráns

2021

Front. Bioeng. Biotechnol.

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Carrier-free immobilization is a key process to develop efficient biocatalysts able to catalyze the cell wall degradation in microalgae where the traditional solid supports cannot penetrate. Thus, the insolubilization of commercial Celluclast®, Alcalase®, and Viscozyme® enzymes by carrier-free immobilization and their application in microalgae pretreatment was investigated. In this study, different precipitants at different ratios (ethanol, acetone, and polyethylene glycol 4000) were tested in the first part of the method, to establish the precipitation conditions. The screening of the best precipitant is needed as it depends on the nature of the enzyme. The best results were studied in terms of immobilization yield, thermal stability, and residual activity and were analyzed using scanning electron microscopy. Moreover, a novel strategy was intended including the three enzymes (combi-CLEAs) to catalyze the enzymatic degradation of Nannochloropsis gaditana microalgal cell wall in one pot. The carrier-free immobilized derivatives were 10 times more stable compared to soluble enzymes under the same. At the best conditions showed its usefulness in the pretreatment of microalgae combined with ultrasounds, facilitating the cell disruption and lipid recovery. The results obtained suggested the powerful application of these robust biocatalysts with great catalytic properties on novel and sustainable biomass such as microalgae to achieve cost-effective and green process to extract valuable bioactive compounds.

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Section: Resultscontrasting

confidence: 72%

Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Cross-Linked Enzyme Aggregates and Their Application in Enzymatic Pretreatment of Microalgae: Comparison Between CLEAs and Combi-CLEAs

Blanco-Llamero

García-García

Señoráns

2021

Front. Bioeng. Biotechnol.

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“…However, the effect of lignocellulosic biomass on laccase activity from the genus Phlebia has not been reported. The laccase of genus Trametes has been extensively studied, and Trametes hirsuta Han 726 used in this study also belongs to one species of genus Trametes (Kołodziejczak-Radzimska et al 2020;Sun et al 2020;Bilal et al 2021;Mejía-Otálvaro et al 2021;Navada and Kulal 2021;Wulandari et al 2021;Zhang et al 2021). However, the laccase of genus Coriolopsis has been less studied, and previous studies were mainly focused on recombinant expression, heterologous expression, and the selection of high production of laccase (Songulashvili et al 2016;Xu et al 2016;Avelar et al 2017;Pinar et al 2017;Glazunova et al 2018).…”

Section: Statistical Analysis Resultsmentioning

confidence: 99%

Laccase activities from three white-rot fungal species isolated from their native habitat in North China using solid-state fermentation with lignocellulosic biomass

Liu

Zhao

et al. 2022

BioRes

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Laccase has huge potential application in all aspects of biotechnology due to the ability of laccase to oxidize a wide range of phenolic and non-phenolic compounds. The future success of such applications requires large amounts of laccase with low costs. White-rot fungi are important groups of laccase production. In this study, three white-rot fungi, Phlebia acerina Han 618, Trametes hirsuta Han 726, and Coriolopsis trogii Han 751, isolated from a native North China habitat, were identified by the method of molecular biology and preliminary screening of the ability of laccase-production by guaiacol selection medium. Then they were fermented on different lignocellulosic biomass. Three species showed consistency in preference of lignocellulosic biomass, and the presence of stalk of Sorghum bicolor was more suitable for secreting laccase. The capacity of laccase secretion from different species was significantly different. The capacity of secreting laccase of C. trogii Han 751 was superior to that of P. acerina Han 618 and T. hirsuta Han 726. The discovery of a new strain with superior capacity of secreting laccase and suitable lignocellulosic biomass were helpful for laying a foundation for the optimization of the fermentation conditions for the highest laccase production, the isolation and purification of laccase, and the industrial application of laccase.

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Dephytinization of wheat and rice bran by cross‐linked enzyme aggregates of Mucor indicus phytase: a viable prospect for food and feed industries

Venkataraman

Vaidyanathan

2022

J Sci Food Agric

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BACKGROUND Novel feeds for improved feed intake and for enhanced nutrient bioavailability have recently attracted attention. Insoluble dietary fibers, especially rice and wheat bran, have generated much interest due to their nutritional value. Incorporating insoluble dietary fiber into diets could be a viable way to maximize the feed conversion ratio. RESULTS Cross‐linked phytase aggregates (CLPA) were synthesized by precipitating enzymes followed by cross‐linking with 5 mmol L−1 glutaraldehyde, yielding 88.24 (U g−1) of enzyme load without the assistance of a proteic feeder. The epitome of the study is the dephosphorylation of wheat bran and rice bran by varying pH, enzyme concentration, and temperature. The highest inorganic phosphorus liberation by 150 U L‐‐1of free phytase was 23.72 (wheat bran) and 48.08 mg g−1 (rice bran) after 12 h of incubation. Furthermore, 150 U L‐1 of CLPA liberated 28.72 (wheat bran) and 52.08 mg g−1 (rice bran) of inorganic phosphorus with an incubation time of 12 h. CONCLUSION Thermostable free phytase was insolubilized to dephosphorylate the agro‐residue, namely, wheat bran and rice bran, to reduce the anti‐nutritional factor (the phytate content) of these insoluble dietary fibers. © 2022 Society of Chemical Industry.

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Development and characterization of cross-linked laccase aggregates (Lac-CLEAs) from Trametes versicolor IBL-04 as ecofriendly biocatalyst for degradation of dye-based environmental pollutants

Cited by 25 publications

References 55 publications

Cross-Linked Enzyme Aggregates and Their Application in Enzymatic Pretreatment of Microalgae: Comparison Between CLEAs and Combi-CLEAs

Cross-Linked Enzyme Aggregates and Their Application in Enzymatic Pretreatment of Microalgae: Comparison Between CLEAs and Combi-CLEAs

Laccase activities from three white-rot fungal species isolated from their native habitat in North China using solid-state fermentation with lignocellulosic biomass

Dephytinization of wheat and rice bran by cross‐linked enzyme aggregates of Mucor indicus phytase: a viable prospect for food and feed industries

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