Development and characterization of cell lines of normal mouse bladder epithelial cells and 2-acetylaminofluorene-induced urothelial carcinoma cells grown in monolayer tissue culture

Berky, John J.; Zolotor, Laurence

doi:10.1007/bf02627862

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1982

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“…On this basis the parenchymal cells were well distinguishable from the nonparenchymal cells. A similar observation had been made in cell cultures of the inter mediate lobe [Fazekas et al, 1976], The abundance of such structures on the surface of epithelial cells of other origin has also been described [Berky and Zolotor, 1977;Miettinen et al, 1978].…”

Section: Discussionmentioning

confidence: 57%

Surface Topography of Cultured Anterior Pituitary Cells of the Rat

Fazekas¹,

Bácsy²,

Rappay³

1982

Cells Tissues Organs

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Anterior pituitary cells of the adult rat were studied by scanning electron microscopy after 3, 6 and 8 days in culture. Epithelial (parenchymal) cells and fibroblasts could be differentiated by their characteristic cell surface structures and by their associations with each other. The surface of solitary and grouped epithelial cells is uneven and rich in smaller or larger blebs, wrinkles and microvilli. A few solitary cilia were found on the surface of some cells. The individual cells forming epithelial cell groups adhere to each other with numerous, parallel, slender extensions. The fibroblasts found in monolayers have extensions with various lengths contacting other cells or the inert substratum. Microvilli arise in various frequency from their smooth surface. Transmission electron microscopy confirmed the presence of the surface structures detected in the scanning electron microscope.

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Section: Discussionmentioning

confidence: 57%