Development and characterization of an Fv-1-sensitive retrovirus-packaging system: single-hit titration kinetics observed in restrictive cells

Boone, Lawrence R.; Innes, Cynthia L.; Glover, Paul L.; Linney, Elwood

doi:10.1128/jvi.63.6.2592-2597.1989

Cited by 14 publications

(3 citation statements)

References 46 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently a host gene (Fv1) involved in controlling Friend retrovirus replication was cloned [100]. Of this Fv1 gene it was already known that it acts on Friend leukemia virus at a stage after entry into the target cell but before integration by direct interaction between the Fv1 gene product and the capsid protein [101][102][103]. The presence of the Fv1 gene product was shown to reduce viral titers up to 1,000-fold [104].…”

Section: Intracellular Half-life Of Retrovirusmentioning

confidence: 99%

Retroviral Stem Cell Gene Therapy

et al. 1997

View full text Add to dashboard Cite

Long-term in vivo gene transfer studies in mice have shown that recombinant murine retroviruses are able to infect murine hemopoietic stem cells with high efficiency. Taken together the results indicated that the proviral structure was present at high frequency in circulating hemopoietic cells resulting in significant expression levels. Because of the success of these murine studies, it was believed that gene therapy would soon be applicable to treat a wide variety of congenital or acquired human diseases associated with the hemopoietic system. However, results from gene transfer studies in nonhuman primates and first human clinical trails have indicated that murine retrovirus infection of primate hemopoietic stem cells is inefficient. Although there are essential differences between the murine and primate gene therapy studies with respect to the recombinant viruses and transduction protocols used, these differences cannot solely account for the differences observed in infection efficiency. Therefore, in recent years effort has been spent on the identification of factors limiting retroviral transduction of primate hemopoietic stem cells. Increasing knowledge concerning hemopoiesis and retroviral infection has helped in identifying a number of limiting factors. Novel transduction strategies and tools have been generated which attempt to circumvent these limiting factors. These factors as well as the strategies that showed increased retroviral infection of primate hemopoietic stem cells will be discussed.

show abstract

Section: Intracellular Half-life Of Retrovirusmentioning

confidence: 99%

Retroviral Stem Cell Gene Therapy

et al. 1997

View full text Add to dashboard Cite

show abstract

“…Although the multihit requirement for infectivity has been extensively documented, some investigators have reported single-hit infectivity in restrictive cells (16,29). We have recently described the development of an N-tropic (i.e., Fv-lb_sensitive) packaging cell line and have observed restriction (approximately 100-fold) in Fv-lb cells infected with a retrovirus vector produced by it (4). The titration pattern was always single-hit, in contrast to our experience measuring infectivity of N-tropic MuLV in restrictive cells using the XC plaque assay in restrictive cells.…”

mentioning

confidence: 99%

“…The Fv-J N-tropic retrovirus packaging mutant provirus was derived from pWN41 (5), a molecular clone of WN1802N (14,22). The resulting packaging cell line, N-Pac, and the retrovirus vector conferring G418 resistance, RV-neo (provided by E. Linney), have been described previously (4). B-tropic MuLV was derived from pWB5 (5), a molecular clone of WN1802B (14,22).…”

mentioning

confidence: 99%

Abrogation of Fv-1 restriction by genome-deficient virions produced by a retrovirus packaging cell line

Boone

Innes

Heitman

1990

J Virol

Self Cite

View full text Add to dashboard Cite

The Fv_lb-mediated restriction of N-tropic retrovirus vector infection of BALB/3T3 cells was partially abrogated by prior infection with N-tropic murine leukemia virus. Likewise, abrogation of the FV_lb restriction of N-tropic murine leukemia virus replication was accomplished by prior infection with genome-deficient virions produced by an N-tropic murine leukemia virus packaging cell line. The latter observation suggests that the Fv-1 target in genome-deficient virions abrogates Fv-1 restriction in the absence of any viral genomedirected processes.

show abstract

Genetic map of the region surrounding the retrovirus restriction locus, Fv1, on mouse Chromosome 4

Stoye¹,

Kaushik²,

Jeremiah

et al. 1995

Mammalian Genome

View full text Add to dashboard Cite

The Friend virus susceptibility-1 (Fv1) gene maps to mouse Chromosome (Chr) 4 close to a cluster of four endogenous murine leukemia viruses (MLVs). To investigate the feasibility of cloning Fv1 by a positional approach, we have performed an extensive genetic analysis of this region of Chr 4. We have typed 368 backcross mice for the four proviruses, Nppa, Lck, and D4Smh6b. Recombinant animals were screened in a hierarchical fashion with a variety of other markers, including Fv1 and the isozyme marker Gpd1. A detailed genetic map of the region surrounding Fv1 was derived. Three markers, Xmv9, Nppa, and Iap3rc11, were identified that showed no recombination with Fv1. By combining backcross and recombinant inbred strain data, we estimated that Xmv9 and Nppa must lie within 0.6 cM of one another and Fv1.

show abstract

Development and characterization of an Fv-1-sensitive retrovirus-packaging system: single-hit titration kinetics observed in restrictive cells

Cited by 14 publications

References 46 publications

Retroviral Stem Cell Gene Therapy

Retroviral Stem Cell Gene Therapy

Abrogation of Fv-1 restriction by genome-deficient virions produced by a retrovirus packaging cell line

Genetic map of the region surrounding the retrovirus restriction locus, Fv1, on mouse Chromosome 4

Contact Info

Product

Resources

About