Development and characterization of amphotericin B nanoemulsion-loaded mucoadhesive gel for treatment of vulvovaginal candidiasis

Patil, Mrunal U.; Rajput, Amarjitsing; Belgamwar, Veena S.; Chalikwar, Shailesh S.

doi:10.1016/j.heliyon.2022.e11489

Cited by 6 publications

(5 citation statements)

References 59 publications

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“…We chose to employ p-407 for its in situ gelling potential, while adding x-gum to ensure a long permanence on the vaginal mucosa in contact with body fluids. Previous studies showed that below 15% w/w, p-407 solutions undergo transition to gels at temperatures above 60 • C, whereas p-407 solutions above 18% w/v convert into stiff gels at 20-25 • C [28][29][30][31][32][33][34][35][36][37][38][39]. Thus, we solubilized p-407 15 and 18% w/w in lactate buffer (G15 and G18, respectively), to obtain pH 4.5 ± 0.1, compatible with vaginal milieu [46].…”

Section: Gel Formulative Studymentioning

confidence: 99%

“…Rheological measurements of hydrogels were performed at 20 or 35 • C with an AR-G2 controlled-stress rotational rheometer (TA Instruments, New Castle, DE, USA) [32]. The geometry used was a sandblasted titanium plate-plate (diameter 40 mm) with a 100 µm gap.…”

Section: Rheological Measurementsmentioning

confidence: 99%

“…The biodegradable character of these gels avoids the need of the formulation removal, while the gel viscosity allows a long permanence of the loaded drug on the vaginal mucosa [23]. In this respect, to treat C. albicans infections, some authors previously proposed p-407-based gels to administer clotrimazole, fluconazole, econazole, oxiconazole, or amphotericin B on skin and vaginal mucosa [29][30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

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Calcitermin-Loaded Smart Gels Activity against Candida albicans: A Preliminary In Vitro Study

Bellotti

D’Accolti

Pula

et al. 2023

Gels

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Calcitermin is an antimicrobial peptide of 15 amino acids found in human nasal fluid characterized by antifungal and antibacterial properties. Candida albicans is the most common human fungal pathogen affecting many tissues, such as vaginal mucosa. In this study a formulation suitable for calcitermin administration on vaginal mucosa was developed for the treatment of fungal infections. To favor topical application, mucosal adhesion, and permanence, gels based on poloxamer 407 and xanthan gum were designed and compared with regard to their rheological behavior, erosion, and leakage. The selected gel was loaded with calcitermin, whose release kinetic was evaluated in vitro by Franz cells. An antifungal activity assay was conducted to assess the calcitermin anticandidal potential and the effect of its inclusion in the selected gel. The rheological study revealed the elastic and viscous moduli behavior as a function of poloxamer 407 and xanthan gum concentration. Xanthan gum presence decreased the transition temperature of the gel, while prolonging its erosion and leakage. Particularly, poloxamer 407, 18% and xanthan gum 0.4% were chosen. The calcitermin loading in the selected gel resulted in a transparent and homogeneous formulation and in a 4-fold decrease of the release rate with respect to the calcitermin solution, as evidenced by Franz cell study. The anticandidal activity tests demonstrated that calcitermin-loaded gel was more active against Candida albicans with respect to the peptide solution.

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Section: Gel Formulative Studymentioning

confidence: 99%

Section: Rheological Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Calcitermin-Loaded Smart Gels Activity against Candida albicans: A Preliminary In Vitro Study

Bellotti

D’Accolti

Pula

et al. 2023

Gels

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“…In a recent study focusing on the formulation of a nanoemulsion containing celecoxib, it was found that MCTs oil can effectively solubilize highly lipophilic drugs such as celecoxib 25) . Similarly, in the study by Patil et al 26) , Capmul ® MCM, a mono-diglyceride of medium chain fatty acids (mainly caprylic and capric acid) was employed as a key component in a mucoadhesive gel formulation to enhance the solubility and permeability of amphotericin B; a highly lipophilic drug. These cases demonstrate the advantages of using MCTs in DDS as they can improve the solubility of drugs that might be a challenge in formulation processes.…”

Section: Introductionmentioning

confidence: 99%

Medium-Chain Triacylglycerols (MCTs) and Their Fractions in Drug Delivery Systems : A Systematic Review

Zulfakar,

Pubadi,

Ibrahim

et al. 2024

J. Oleo Sci.

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the drug across the biological membrane to the site of action, are a part of the drug delivery process. DDS employs various types of routes which encompass mouth or oral routes, topical routes, transdermal routes, and parenteral routes such as the intravenous (IV) , intramuscular (IM) , subcutaneous (SC) , and pulmonary routes 3,4) . Topical drug delivery involves the direct application of a drug to the skin surface to achieve localized therapeutic effects. Transdermal drug delivery involves administering a drug to the skin to pass through the skin layers and infiltrate the bloodstream for systemic effects throughout the body 5,6) .

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“…In some instances, surfactants can be combined with co‐surfactants like propylene glycol (PG), polyethylene glycol 400 (PEG400), poly glyceryl oleate (PGO), etc. to enhance the stability of NEs [13,14] …”

Section: Introductionmentioning

confidence: 99%

Optimizing Preparation of Fish Scale Collagen Peptide/Sacha Inchi (Plukenetia volubilis L.) Seed Oil Nanoemulsion

Vu,

Mai

et al. 2024

ChemistrySelect

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This study presents results of extracting hydrolyzed collagen peptide from fresh‐water fish scale and using it to prepare a nanoemulsion based on Sacha inchi (Plukenetia volubilis L.) seed oil and collagen peptide. The sacha inchi seed oil is extracted from the seeds of Sacha Inchi (abbreviated as sachi) plant and contains approximate 45 % triple omega 3–6‐9 (ω‐3, ω‐6, ω‐9) content. Pseudo‐ternary phase diagrams of three components: aqueous collagen solution, sachi seed oil, and surfactant/co‐surfactant (Smix) have been constructed. The parameters including the composition of Smix, sachi seed oil, and aqueous collagen solution have been selected to optimize the technology factors for the emulsion process. A quadratic regression equation was chosen, and experimental planning following the Box‐Behnken model using response surface methodology was used to optimize the preparation conditions for the nanoemulsion. The average droplet size of the emulsion systems is the object function for optimization. The Smix is a mixture of surfactant (Tween80) and propylene glycol (PG) with different ratios of Tween80/PG of 1/1, 2/1, 3/1, and 4/1, corresponding to the formation of four emulsion systems, code 1, 2, 3, 4, respectively. The optimal parameters for each emulsion system are different, however, the aqueous collagen solution contains a higher proportion in emulsions. Under the optimal conditions, the predicted values of the objective function of emulsions code 1–code 4 ranged from 95.62 to 185.14 nm, increasing together with the Tween80 ratio in Smix. The experiment values of the objective function of emulsions code 1–code 4 prepared under the optimal conditions are 104.22, 133.70, 159.27, and 207.87 nm, respectively. These values are close to the predicted values, suggesting that the suitability of the experiment and theory. Additionally, the stability of the nanoemulsion prepared under the optimal conditions is also evaluated. A stable nanoemulsion is potentially for loading nutraceuticals and vitamins for applications in personal health care and medicinal fields.

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Development and characterization of amphotericin B nanoemulsion-loaded mucoadhesive gel for treatment of vulvovaginal candidiasis

Cited by 6 publications

References 59 publications

Calcitermin-Loaded Smart Gels Activity against Candida albicans: A Preliminary In Vitro Study

Calcitermin-Loaded Smart Gels Activity against Candida albicans: A Preliminary In Vitro Study

Medium-Chain Triacylglycerols (MCTs) and Their Fractions in Drug Delivery Systems : A Systematic Review

Optimizing Preparation of Fish Scale Collagen Peptide/Sacha Inchi (Plukenetia volubilis L.) Seed Oil Nanoemulsion

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