Development and Characterization of Acellular Porcine Corneal Matrix Using Sodium Dodecylsulfate

Zhou, Yang; Wu, Zheng; Ge, Jian; Park, Wan; Li, Naiyang; Xiang, Peng; Gao, Qianying; Wang, Zhichong

doi:10.1097/ico.0b013e3181dc8184

Cited by 40 publications

(36 citation statements)

References 35 publications

(28 reference statements)

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“…Alternatively, efforts by a number of investigators have focused on the use of de-cellularized, chemically-treated pig corneas as an alternative to human donor corneal tissue [10][11][12][13]. While this approach has shown promising results in animal models, the standardized procurement, decellularization methods, storage procedures, and material certification for human use are lacking and can lead to varying outcomes, for example with respect to transparency of the final constructs [10,13].…”

Section: Introductionmentioning

confidence: 99%

“…The surgery minimizes disturbance of the epithelium and maintains corneal shape and curvature without suturing, thereby avoiding stimulation of an aggressive wound healing response. Additionally, FLISK can be considered a therapeutic procedure for replacement of diseased or damaged native stroma, in contrast to micropocket insertion models [10,12,13] where native tissue is not excised thus leading to an unnatural refraction and stromal architecture, inflammation, and the need for surgical suturing and use of postoperative steroids. In this study, it was found that the BPC implants maintained the shape and structure of the host cornea while supporting rapid healing and a limited in-growth of host stromal cells, which began to degrade the implanted collagen scaffold and produce new host collagen in less than 8 weeks.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Koulikovska

Rafat

Petrovski

et al. 2015

Tissue Engineering Part A

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Severe shortage of donor corneas for transplantation, particularly in developing countries, has prompted the advancement of bioengineered tissue alternatives. Bioengineered corneas that can withstand transplantation while maintaining transparency and compatibility with host cells, and that are additionally amenable to standardized, low-cost mass production, are sought. In this study, a bioengineered porcine construct (BPC) was developed to function as a biodegradable scaffold to promote corneal stromal regeneration by host cells. Using high purity medical-grade type I collagen, high 18% collagen content and optimized EDC-NHS crosslinker ratio, BPCs were fabricated into hydrogel corneal implants with over 90% transparency and fourfold increase in strength and stiffness compared to previous versions. Remarkably, optical transparency was achieved despite the absence of collagen fibril organization at the nanoscale. In vitro testing indicated the BPC supported confluent human epithelial and stromal-derived mesenchymal stem cell populations. With a novel femtosecond laser-assisted corneal surgical model in rabbits, cell-free BPCs were implanted in vivo in the corneal stroma of 10 rabbits over an 8 week period. In vivo, transparency of implanted corneas was maintained throughout the postoperative period, while healing occurred rapidly without inflammation and without the use of postoperative steroids. BPC implants had a 100% retention rate at 8 weeks, when host stromal cells began to migrate into implants. Direct histochemical evidence of stromal tissue regeneration was observed by means of migrated host cells producing new collagen from within the implants. This study indicates that a cost-effective BPC extracellular matrix equivalent can incorporate cells passively to initiate regenerative healing of the corneal stroma, and is compatible with human stem or organ-specific cells for future therapeutic applications as a stromal replacement for treating blinding disorders of the cornea.3

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Koulikovska

Rafat

Petrovski

et al. 2015

Tissue Engineering Part A

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“…21 In the cornea, decellularized xenograft matrices have been studied primarily for stromal replacement. [22][23][24][25][26][27][28] In this study, we undertook a tissue-engineering approach to evaluate methods for removing cells from human cadaver corneas while maintaining the integrity of the basement membrane and the stromal matrix. The optimized protocol resulted in a decellularized cornea fully capable of supporting the growth and differentiation of human corneal epithelial and stromal cells in vitro.…”

Section: Introductionmentioning

confidence: 99%

Decellularized Human Cornea for Reconstructing the Corneal Epithelium and Anterior Stroma

Shafiq

Gemeinhart

Yue

et al. 2012

Tissue Engineering Part C: Methods

108

124

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“…fibres and matrix. Parameter E can be used to describe the combined response of the corneal fibres and matrix (Dunn andSilver 1983, Edmund 1988), whereas when appropriately transformed, and τi can describe corneal matrix behaviour (Dunn and Silver 1983, Crabb et al 2006, Zhou et al 2011.…”

Section: Methodsmentioning

confidence: 99%

A hybrid approach to determining cornea mechanical properties in vivo using a combination of nano-indentation and inverse finite element analysis

Abyaneh

Wildman

Ashcroft

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

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A hybrid approach to determining cornea mechanical properties in vivo using a combination of nano-indentation and inverse finite element analysis approach. A validation experiment using nano-indentation showed that an isotropic GM model was insufficient for describing the corneal material behaviour when exposed to a complex stress state. A new technique was proposed for determining the properties, using a combination of nano-indentation experiment, an isotropic and orthotropic GM model and inverse finite element method. The good agreement using this method suggests that this is a promising technique for measuring material properties in vivo and further work should focus on the reliability of the approach in

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Development and Characterization of Acellular Porcine Corneal Matrix Using Sodium Dodecylsulfate

Cited by 40 publications

References 35 publications

Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Enhanced Regeneration of Corneal Tissue via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique

Decellularized Human Cornea for Reconstructing the Corneal Epithelium and Anterior Stroma

A hybrid approach to determining cornea mechanical properties in vivo using a combination of nano-indentation and inverse finite element analysis

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