Development and characterization of a skin cell line (SGA) from the mosquitofish Gambusia affinis and its susceptibility to fish Betanodavirus

Development and characterization of a spleen cell line from yellowfin seabream Acanthopagrus latus and its susceptibility to Mandarinfish ranavirus

“…Most of the commercially available transfection reagents were designed for mammalian cells. Different incubated times and temperatures may cause low transfection efficiency in fish cell lines (Jyotsna et al, 2020). Such a problem will be attempted to be improved in future work.…”

“…One of the major applications of a fish cell line is to carry out virology experiments (Cheng et al, 2010; Rougee et al, 2007). Viral diseases have caused considerable economic losses in aquaculture, and cell lines are crucial for viral detection and isolation (Jyotsna et al, 2020). Chang et al studied the susceptibility and replication rate of six viruses in the cell line of Lates calcarifer and found that all of them could proliferate in the cell line, but only five viruses could observe significant CPEs (Qin et al, n.d.).…”

“…The cell lines from different fish have been used as an important in‐vitro model in virology. Generally, cell lines are commonly used as a tool to isolate viruses and verify the virus–host interactions, and it has been widely adopted in the field of virology (Jyotsna et al, 2020; Zhong et al, 2023). To date, continuous cell lines derived from liver, fin and brain tissues of A. latus have been established and characterized (Fu et al, 2022; Jia et al, 2022; Li et al, 2022), but the cell line from important immune tissues remains unexplored.…”

Development and characterization of a skin cell line from Chinese perch (Siniperca chuatsi) and its application in aquatic animal viruses

“…ADRV and RGV belongs to the genus Ranavirus in the family Iridoviridae, which also contains the ranavirus infecting S. chuatsi (Fu et al, 2017). PORV is another rhabdovirus which is isolated from diseased Paralichthys olivaceus and also poses a great threat to aquaculture like SCRV (Jyotsna, 2020). GCRV is an important aquatic reovirus, which has great harm to freshwater fisheries, especially grass carp aquaculture (Pei et al, 2014).…”

“…The cutaneous tissue of fish is an important physical and physiological barrier against invading pathogens. Although cell lines from skins of some fishes, such as red‐spotted grouper ( Epinephelus akaara ), Ussuri catfish ( Pseudobagrus ussuriensis ), Japanese flounder ( Paralichthys olivaceus ) and mosquitofish ( Gambusia affinis ), have been established (Jyotsna, 2020; Lei et al, 2012; Ou et al, 2014; Wang et al, 2017), the number of cell lines from fish skin remains relatively limited.…”