Development and characterization of a photocurable alginate bioink for three-dimensional bioprinting

H, Mishbak H.; Cooper, Glen M.; Bartolo, Paulo Jorge Da Silva

doi:10.18063/ijb.v5i2.189

Cited by 38 publications

(11 citation statements)

References 33 publications

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“…Photoinitiators are mostly used in the HIPE composition at a concentration range of 0.2-10% (w/w) of the polymer. The concentration of the photoinitiator that is included in the composition of the photocurable resins is reported to have an effect on the rheological properties of the monomer and its gelation time (Mishbak et al, 2019). Interestingly, there are few studies that investigate the photoinitiator type and concentration on the characteristics of PolyHIPEs (Harikrishna et al, 2014).…”

Section: Initiatorsmentioning

confidence: 99%

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims to regenerate critical size defects, which cannot heal naturally, by using highly porous matrices called TE scaffolds made of biocompatible and biodegradable materials. There are various manufacturing techniques commonly used to fabricate TE scaffolds. However, in most cases, they do not provide materials with a highly interconnected pore design. Thus, emulsion templating is a promising and convenient route for the fabrication of matrices with up to 99% porosity and high interconnectivity. These matrices have been used for various application areas for decades. Although this polymer structuring technique is older than TE itself, the use of polymerised internal phase emulsions (PolyHIPEs) in TE is relatively new compared to other scaffold manufacturing techniques. It is likely because it requires a multidisciplinary background including materials science, chemistry and TE although producing emulsion templated scaffolds is practically simple. To date, a number of excellent reviews on emulsion templating have been published by the pioneers in this field in order to explain the chemistry behind this technique and potential areas of use of the emulsion templated structures. This particular review focusses on the key points of how emulsion templated scaffolds can be fabricated for different TE applications. Accordingly, we first explain the basics of emulsion templating and characteristics of PolyHIPE scaffolds. Then, we discuss the role of each ingredient in the emulsion and the impact of the compositional changes and process conditions on the characteristics of PolyHIPEs. Afterward, current fabrication methods of biocompatible PolyHIPE scaffolds and polymerisation routes are detailed, and the functionalisation strategies that can be used to improve the biological activity of PolyHIPE scaffolds are discussed. Finally, the applications of PolyHIPEs on soft and hard TE as well as in vitro models and drug delivery in the literature are summarised.

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Section: Initiatorsmentioning

confidence: 99%

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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“…Some commonly used photo-curable bio-resins include polyethylene glycol acrylate (PEGDA) [62,63] and gelatin methacrylate (GelMA) [62][63][64][65]. Naturallyderived biomaterials such as hyaluronic acid, dextran, and alginate have also been modified with methacrylate groups to form photo-curable bio-resins [66][67][68]. Furthermore, photo-absorber species can be introduced to improve print resolution by reducing the light penetration depth to achieve better control over the print resolution [69][70][71][72].…”

Section: Photo-curable Bio-resin Formulationmentioning

confidence: 99%

Vat polymerization-based bioprinting—process, materials, applications and regulatory challenges

et al. 2020

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Over the years, the field of bioprinting has attracted attention for its highly automated 16 fabrication system that enables the precise patterning of living cells and biomaterials at pre-17 defined positions for enhanced cell-matrix and cell-cell interactions. Notably, vat polymerization 18 (VP)-based bioprinting is an emerging bioprinting technique for various tissue engineering 19 applications due to its high fabrication accuracy. Particularly, different photo-initiators (PIs) are 20 utilized during the bioprinting process to facilitate the crosslinking mechanism for fabrication of 21 high-resolution complex tissue constructs. The advancements in VP-based printing have led to a 22 paradigm shift in fabrication of tissue constructs from cell-seeding of tissue scaffolds (non-23 biocompatible fabrication process) to direct bioprinting of cell-laden tissue constructs 24 (biocompatible fabrication process). This paper, presenting a first-time comprehensive review of 25 the VP-based bioprinting process, provides an in-depth analysis and comparison of the various 26 biocompatible PIs and highlights the important considerations and bioprinting requirements. This 27 review paper reports a detailed analysis of its printing process and the influence of light-based 28 curing modality and PIs on living cells. Lastly, this review also highlights the significance of VP-29 based bioprinting, the regulatory challenges and presents future directions to transform the VP-30 Page 1 of 47 AUTHOR SUBMITTED MANUSCRIPT -BF-102156.R2based printing technology into imperative tools in the field of tissue engineering and regenerative 31 medicine. The readers will be informed on the current limitations and achievements of the VP-32 based bioprinting techniques. Notably, the readers will realize the importance and value of 33 highly-automated platforms for tissue engineering applications and be able to develop objective 34 viewpoints towards this field.

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“…The structures showed low swelling, high degradation, higher mechanical stiffness, increased gelation time and higher mechanical strength with increasing photoinitiator concentration. [ 95 ] In another study, the fabrication process of alginate/carrageenan composite scaffolds by extrusion‐based bioprinting was reported. First, by measuring the shear modulus of different alginate‐based hydrogels, the most suitable concentrations for crosslinking were determined and then with different concentrations of carrageenan, alginate/carrageenan composite hydrogels were prepared and characterized for their rheological properties.…”

Section: Hydrogel‐based Bioinksmentioning

confidence: 99%

Natural and Synthetic Bioinks for 3D Bioprinting

Khoeini

Nosrati

Akbarzadeh

et al. 2021

Advanced NanoBiomed Research

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Bioprinting offers tremendous potential in the fabrication of functional tissue constructs for replacement of damaged or diseased tissues. Among other fabrication methods used in tissue engineering, bioprinting provides accurate control over the construct's geometric and compositional attributes using an automated approach. Bioinks are composed of the hydrogel material and living cells that are critical process variables in the fabrication of functional, mechanically robust constructs. Appropriate cells can be encapsulated in bioinks to create functional tissue structures. Ideal bioinks are required to undergo a sol–gel transition consuming minimal processing time, and a plethora of chemical and physical crosslinking mechanisms are generally exploited to achieve high shape fidelity and construct stability. In contrast, crosslinking of hydrogel material at rapid rates can cause nozzle clogging, and hence, optimization of the bioink is often necessary. Bioinks can be formulated using natural or synthetic biomaterials, alone or in combination of these biomaterials. Herein, the various bioprinting methods are discussed; the natural, synthetic, or hybrid materials used as bioinks are analyzed; and the challenges, limitations, and future directions concerning the bioprinting technique are appraised.

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Development and characterization of a photocurable alginate bioink for three-dimensional bioprinting

Cited by 38 publications

References 33 publications

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Vat polymerization-based bioprinting—process, materials, applications and regulatory challenges

Natural and Synthetic Bioinks for 3D Bioprinting

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