Development and characterization of a poloxamer hydrogel composed of human mesenchymal stromal cells (hMSCs) for reepithelization of skin injuries

Galocha-León, Cristina; Antich, Cristina; Voltes-Martínez, Ana; Marchal, Juan A.; Mallandrich, Mireia; Halbaut, Lyda; Rodríguez-Lagunas, María J.; Souto, Eliana B.; Clares-Naveros, Beatriz; Gálvez-Martín, Patricia

doi:10.1016/j.ijpharm.2023.123535

Cited by 4 publications

(1 citation statement)

References 112 publications

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“…Adipose-derived stem cell (ADSC)-and platelet-rich plasma (PRP)supported hydrogel systems based on methacrylate gelatin (GelMA) and methacrylate fibroin protein (SFMA) have been developed as cell and growth factor delivery carriers for the treatment of pressure ulcers, and have shown good efficacy [110]. In addition, studies have indicated a great potential for using Poloxam hydrogel as a cell carrier to support human mesenchymal stromal cells (hMSCs) [111]. hUCMSCs, human umbilical cord mesenchymal stem cells encapsulated in a functional injectable thermosensitive hydrogel (chitosan/sodium glycerophosphite/cellulose nanocrystalline, CS/GP/CNC), can be used to repair full-layer skin wounds and significantly accelerate wound closure, microcirculation, tissue remodeling, re-epithelialization, and hair follicle regeneration [112].…”

Section: Stem Cell Delivery Into Skin Woundsmentioning

confidence: 99%

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Wu,

Sun,

et al. 2024

Biomedicines

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Mesenchymal stem cells (MSCs) have been recognized as a cell therapy with the potential to promote skin healing. MSCs, with their multipotent differentiation ability, can generate various cells related to wound healing, such as dermal fibroblasts (DFs), endothelial cells, and keratinocytes. In addition, MSCs promote neovascularization, cellular regeneration, and tissue healing through mechanisms including paracrine and autocrine signaling. Due to these characteristics, MSCs have been extensively studied in the context of burn healing and chronic wound repair. Furthermore, during the investigation of MSCs, their unique roles in skin aging and scarless healing have also been discovered. In this review, we summarize the mechanisms by which MSCs promote wound healing and discuss the recent findings from preclinical and clinical studies. We also explore strategies to enhance the therapeutic effects of MSCs. Moreover, we discuss the emerging trend of combining MSCs with tissue engineering techniques, leveraging the advantages of MSCs and tissue engineering materials, such as biodegradable scaffolds and hydrogels, to enhance the skin repair capacity of MSCs. Additionally, we highlight the potential of using paracrine and autocrine characteristics of MSCs to explore cell-free therapies as a future direction in stem cell-based treatments, further demonstrating the clinical and regenerative aesthetic applications of MSCs in skin repair and regeneration.

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Section: Stem Cell Delivery Into Skin Woundsmentioning

confidence: 99%

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Wu,

Sun,

et al. 2024

Biomedicines

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Human mesenchymal stromal cells-laden crosslinked hyaluronic acid-alginate bioink for 3D bioprinting applications in tissue engineering

Galocha-León,

Antich,

Voltes-Martínez

et al. 2024

Drug Deliv. and Transl. Res.

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Three-dimensional (3D) bioprinting is considered one of the most advanced tools to build up materials for tissue engineering. The aim of this work was the design, development and characterization of a bioink composed of human mesenchymal stromal cells (hMSC) for extrusion through nozzles to create these 3D structures that might potentially be apply to replace the function of damaged natural tissue. In this study, we focused on the advantages and the wide potential of biocompatible biomaterials, such as hyaluronic acid and alginate for the inclusion of hMSC. The bioink was characterized for its physical (pH, osmolality, degradation, swelling, porosity, surface electrical properties, conductivity, and surface structure), mechanical (rheology and printability) and biological (viability and proliferation) properties. The developed bioink showed high porosity and high swelling capacity, while the degradation rate was dependent on the temperature. The bioink also showed negative electrical surface and appropriate rheological properties required for bioprinting. Moreover, stress-stability studies did not show any sign of physical instability. The developed bioink provided an excellent environment for the promotion of the viability and growth of hMSC cells. Our work reports the first-time study of the effect of storage temperature on the cell viability of bioinks, besides showing that our bioink promoted a high cell viability after being extruded by the bioprinter. These results support the suggestion that the developed hMSC-composed bioink fulfills all the requirements for tissue engineering and can be proposed as a biological tool with potential applications in regenerative medicine and tissue engineering. Graphical abstract

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Fabrication of carboxymethyl cellulose-based thermo-sensitive hydrogels and inhibition of corneal neovascularization

Yang,

Nan,

Zhang

et al. 2024

International Journal of Biological Macromolecules

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Development and characterization of a poloxamer hydrogel composed of human mesenchymal stromal cells (hMSCs) for reepithelization of skin injuries

Cited by 4 publications

References 112 publications

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Human mesenchymal stromal cells-laden crosslinked hyaluronic acid-alginate bioink for 3D bioprinting applications in tissue engineering

Fabrication of carboxymethyl cellulose-based thermo-sensitive hydrogels and inhibition of corneal neovascularization

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