Development and Characterization of a 3D Printed, Keratin-Based Hydrogel

Placone, Jesse K.; Navarro, Javier; Laslo, Gregory W.; Lerman, Max J.; Gabard, Alexis R.; Herendeen, Gregory J.; Falco, Erin E.; Tomblyn, Seth; Burnett, Luke; Fisher, John P.

doi:10.1007/s10439-016-1621-7

Cited by 92 publications

(73 citation statements)

References 31 publications

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“…The keratin-based photocrosslinkable resin was prepared as previously reported [32]. Briefly, keratin was dissolved overnight in phosphate buffered saline (PBS, pH 7.4) at a concentration of 4% wt/vol, and then mixed with a photosensitive solution at a 4:1 ratio.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, extracellular “hard” keratin has been successfully used in the treatment of dermal burn wounds on animal models (split-thickness burns in mice, rats [30], or pigs [31]) and on clinical patients (split-thickness burns <10% of total body surface area [22]). We previously reported a method for three-dimensional (3D) printing hair-derived keratin hydrogels using a riboflavin-sodium persulfate-hydroquinone (initiator-catalyst-inhibitor) photosensitive resin [32]. Ultra-violet (UV) light was used to form dityrosine bonds and photocrosslink keratin on a lithography-based 3D printer [32].…”

Section: Introductionmentioning

confidence: 99%

“…We previously reported a method for three-dimensional (3D) printing hair-derived keratin hydrogels using a riboflavin-sodium persulfate-hydroquinone (initiator-catalyst-inhibitor) photosensitive resin [32]. Ultra-violet (UV) light was used to form dityrosine bonds and photocrosslink keratin on a lithography-based 3D printer [32]. …”

Section: Introductionmentioning

confidence: 99%

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Development of keratin-based membranes for potential use in skin repair

et al. 2019

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The layers in skin determine its protective and hemostasis functions. This layered microstructure cannot be naturally regenerated after severe burns; we aim to reconstruct it using guided tissue regeneration (GTR). In GTR, a membrane is used to regulate tissue growth by stopping fast-proliferating cells and allowing slower cells to migrate and reconstruct specialized microstructures. Here, we proposed the use of keratin membranes crosslinked via dityrosine bonding. Variables from the crosslinking process were grouped within an energy density (ED) parameter to manufacture and evaluate the membranes. Sol fraction, spectrographs, and thermograms were used to quantify the non-linear relation between ED and the resulting crosslinking degree (CD). Mechanical and swelling properties increased until an ED threshold was reached; at higher ED, the CD and properties of the membranes remained invariable indicating that all possible dityrosine bonds were formed. Transport assays showed that the membranes allow molecular diffusion; low ED membranes retain solutes within their structure while the high ED samples allow higher transport rates indicating that uncrosslinked proteins can be responsible of reducing transport. This was confirmed with lower transport of adipogenic growth factors to stem cells when using low ED membranes; high ED samples resulted in increased production of intracellular lipids. Overall, we can engineer keratin membranes with specific CD, a valuable tool to tune microstructural and transport properties.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of keratin-based membranes for potential use in skin repair

et al. 2019

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“…Images at the end of the flow chart represent achievable design parameters using the specified print approach and material. More information on PPF, GelMA, PCL, and keratin is available in recently published studies [97–99]. …”

Section: Figurementioning

confidence: 99%

3D printing for the design and fabrication of polymer-based gradient scaffolds

et al. 2017

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To accurately mimic the native tissue environment, tissue engineered scaffolds often need to have a highly controlled and varied display of three-dimensional (3D) architecture and geometrical cues. Additive manufacturing in tissue engineering has made possible the development of complex scaffolds that mimic the native tissue architectures. As such, architectural details that were previously unattainable or irreproducible can now be incorporated in an ordered and organized approach, further advancing the structural and chemical cues delivered to cells interacting with the scaffold. This control over the environment has given engineers the ability to unlock cellular machinery that is highly dependent upon the intricate heterogeneous environment of native tissue. Recent research into the incorporation of physical and chemical gradients within scaffolds indicates that integrating these features improves the function of a tissue engineered construct. This review covers recent advances on techniques to incorporate gradients into polymer scaffolds through additive manufacturing and evaluate the success of these techniques. As covered here, to best replicate different tissue types, one must be cognizant of the vastly different types of manufacturing techniques available to create these gradient scaffolds. We review the various types of additive manufacturing techniques that can be leveraged to fabricate scaffolds with heterogeneous properties and discuss methods to successfully characterize them.

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“…Different classes of hydrogels have been employed as parts of bioink systems used in tissue/organ bioprinting. 41,43,45,55,62 A promising approach to simultaneously comply with the numerous requirements that AM techniques and bioinks must satisfy to guarantee optimal tissue quality and maximum tissue complexity is to combine various AM technologies and bioinks to benefit from the best aspects of different approaches. Recent application of this pragmatic approach has produced some promising results.…”

Section: Tissue and Organ Equivalentsmentioning

confidence: 99%

Additive Manufacturing of Biomaterials, Tissues, and Organs

2016

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Abstract-The introduction of additive manufacturing (AM), often referred to as three-dimensional (3D) printing, has initiated what some believe to be a manufacturing revolution, and has expedited the development of the field of biofabrication. Moreover, recent advances in AM have facilitated further development of patient-specific healthcare solutions. Customization of many healthcare products and services, such as implants, drug delivery devices, medical instruments, prosthetics, and in vitro models, would have been extremely challenging-if not impossible-without AM technologies. The current special issue of the Annals of Biomedical Engineering presents the latest trends in application of AM techniques to healthcare-related areas of research. As a prelude to this special issue, we review here the most important areas of biomedical research and clinical practice that have benefited from recent developments in additive manufacturing techniques. This editorial, therefore, aims to sketch the research landscape within which the other contributions of the special issue can be better understood and positioned. In what follows, we briefly review the application of additive manufacturing techniques in studies addressing biomaterials, (re)generation of tissues and organs, disease models, drug delivery systems, implants, medical instruments, prosthetics, orthotics, and AM objects used for medical visualization and communication.

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Development and Characterization of a 3D Printed, Keratin-Based Hydrogel

Cited by 92 publications

References 31 publications

Development of keratin-based membranes for potential use in skin repair

Development of keratin-based membranes for potential use in skin repair

3D printing for the design and fabrication of polymer-based gradient scaffolds

Additive Manufacturing of Biomaterials, Tissues, and Organs

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