Development and characterization of 21 novel microsatellite markers for the Australian smelt<i>Retropinna semoni</i>(Weber, 1895)

Islam, Md Rakeb-Ul; Schmidt, D. J.; Hughes, Jane

doi:10.1111/jai.13391

Cited by 6 publications

(5 citation statements)

References 20 publications

(36 reference statements)

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“…Genomic DNA was extracted from fin tissue using the DNeasy Blood and Tissue kits (Qiagen, Germantown, MD, USA) following the manufacturer’s directions. Microsatellite markers developed for R. semoni were amplified and genotyped using primers developed by Islam, Schmidt & Hughes (2017) . Ten loci were screened across all individuals.…”

Section: Methodsmentioning

confidence: 99%

Patterns of genetic structuring at the northern limits of the Australian smelt (Retropinna semoni) cryptic species complex

Islam

Schmidt

Crook

et al. 2018

PeerJ

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Freshwater fishes often exhibit high genetic population structure due to the prevalence of dispersal barriers (e.g., waterfalls) whereas population structure in diadromous fishes tends to be weaker and driven by natal homing behaviour and/or isolation by distance. The Australian smelt (Retropinnidae: Retropinna semoni) is a native fish with a broad distribution spanning inland and coastal drainages of south-eastern Australia. Previous studies have demonstrated variability in population genetic structure and movement behaviour (potamodromy, facultative diadromy, estuarine residence) across the southern part of its geographic range. Some of this variability may be explained by the existence of multiple cryptic species. Here, we examined genetic structure of populations towards the northern extent of the species’ distribution, using ten microsatellite loci and sequences of the mitochondrial cyt b gene. We tested the hypothesis that genetic connectivity among rivers should be low due to a lack of dispersal via the marine environment, but high within rivers due to dispersal. We investigated populations corresponding with two putative cryptic species, SEQ-North (SEQ-N), and SEQ-South (SEQ-S) lineages occurring in south east Queensland drainages. These two groups formed monophyletic clades in the mtDNA gene tree and among river phylogeographic structure was also evident within each clade. In agreement with our hypothesis, highly significant overall FST values suggested that both groups exhibit very low dispersal among rivers (SEQ-S FST = 0.13; SEQ-N FST= 0.27). Microsatellite data indicated that connectivity among sites within rivers was also limited, suggesting dispersal may not homogenise populations at the within-river scale. Northern groups in the Australian smelt cryptic species complex exhibit comparatively higher among-river population structure and smaller geographic ranges than southern groups. These properties make northern Australian smelt populations potentially susceptible to future conservation threats, and we define eight genetically distinct management units along south east Queensland to guide future conservation management. The present findings at least can assist managers to plan for effective conservation and management of different fish species along coastal drainages of south east Queensland, Australia.

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Section: Methodsmentioning

confidence: 99%

Patterns of genetic structuring at the northern limits of the Australian smelt (Retropinna semoni) cryptic species complex

Islam

Schmidt

Crook

et al. 2018

PeerJ

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was extracted from fin tissue using the DNeasy Blood and Tissue kit (Qiagen) following the manufacturer's directions. Microsatellite markers developed for R. semoni were amplified and genotyped using primers developed by Islam, Schmidt & Hughes (2017). Ten loci were screened across all individuals.…”

Section: Molecular Methodsmentioning

confidence: 99%

Patterns of genetic structuring at the northern limits of the Australian smelt (Retropinna semoni) cryptic species complex

Islam

Schmidt

Crook

et al. 2017

Preprint

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Freshwater fishes often exhibit high genetic population structure due to the prevalence of dispersal barriers (e.g., waterfalls) whereas population structure in diadromous fishes tends to be weaker and driven by natal homing behaviour and/or isolation by distance. The Australian smelt (Retropinninae: Retropinna semoni) is a facultatively diadromous fish with a broad distribution spanning inland and coastal drainages of south-eastern Australia. Previous studies have demonstrated variability in population genetic structure and movement behaviour (potamodromy, facultative diadromy, estuarine residence) across the southern part of its geographic range. Some of this variability may be explained by the existence of multiple cryptic species. Here, we examined genetic structure of populations at the northern extent of the species’ distribution, using ten microsatellite loci and sequences of the mitochondrial cyt b gene. We tested the hypothesis that connectivity among rivers should be low due to a lack of dispersal via the marine environment, but high within rivers due to potamodromous behaviour. We investigated populations corresponding with two putative cryptic species, the South East Queensland (SEQ), and Central East Queensland (CEQ) lineages. In agreement with our hypothesis, highly significant overall FST values suggested that both groups exhibit very low dispersal among rivers (SEQ FST = 0.13; CEQ FST = 0.30). The two putative cryptic species, formed monophyletic clades in the mtDNA gene tree and among river phylogeographic structure was also evident within clades. Microsatellite data indicated that connectivity among sites within rivers was also limited, suggesting potamodromous behaviour does not homogenise populations at the within-river scale. Overall, northern groups in the smelt cryptic species exhibit higher among-river population structure and smaller geographic ranges than southern groups. These properties make northern Australian smelt populations potentially susceptible to future conservation threats, and we define eight genetically distinct management units to guide future conservation management.

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“…Furthermore, the researchers that have developed the microsatellites of the Amur sturgeon provided no data on the polymorphic information content and the Hardy-Weinberg departure value in this species. In this study, we report a set of novel crossspecies microsatellite markers from Chinese sturgeon developed by next-generation sequencing technology that can be useful for the research in population genetics, conservation genetics and evolution of Amur sturgeon (Vega-Retter & Véliz, 2015;Xie, Shao, Zhang, & Peng, 2015;J. Zhao, Zhao, & Peng, 2014).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Hu¹,

Liu²,

Yang³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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In the present study, twenty-two microsatellites was developed for Amur sturgeon (Acipenser schrenckii). All of them were polymorphic with 2 to 7 alleles per locus and the total number of alleles is 106. The expected heterozygosity (HE), observed heterozygosity (HO), polymorphic information content (PIC), Hardy-Weinberg departure value (d) and Shannon-Weiner Diversity Indices (H') ranged from 0.35 to 0.84, from 0.46 to 1, from 0.29 to 0.82, from 0.14 to 0.49, and from 0.53 to 1.89 respectively. The microsatellites described here should be useful for the research in population genetics, conservation genetics and evolution of Amur sturgeon.

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Development and characterization of 21 novel microsatellite markers for the Australian smeltRetropinna semoni(Weber, 1895)

Cited by 6 publications

References 20 publications

Patterns of genetic structuring at the northern limits of the Australian smelt (Retropinna semoni) cryptic species complex

Patterns of genetic structuring at the northern limits of the Australian smelt (Retropinna semoni) cryptic species complex

Patterns of genetic structuring at the northern limits of the Australian smelt (Retropinna semoni) cryptic species complex

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