Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing

Esquivel-Bobadilla, Sarai; Garza, Oscar Adrián Lozano; León, Francisco J. Garcı́a de; Barriga-Sosa, Irene de los A.; Jiménez, María-Luisa

doi:10.1007/s12686-012-9782-7

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Conservation Genet Resour

2012

DOI: 10.1007/s12686-012-9782-7

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Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing

Sarai Esquivel-Bobadilla

Oscar Adrián Lozano Garza

Francisco J. Garcı́a de León

et al.

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Cited by 3 publications

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“…However, the technical and economic effort required for developing de novo SSRs in organisms for which no or few genomic resources are available (the so-called non-model organisms) has, until recently, prohibited wide implementation. The recent emergence of next-generation sequencing technologies has reduced the economic and technical difficulties associated with developing SSRs (Santana et al 2009), and has boosted their usage in a wide range of organisms, including spiders (Esquivel-Bobadilla et al 2013;Parmakelis et al 2013), a group in which the development and application of microsatellites has been limited (Brewer et al 2014),…”

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Development of novel microsatellite markers for the spider genusLoxosceles(Sicariidae) using next-generation sequencing

Planas¹,

Bernaus²,

Ribera³

2014

Journal of Arachnology

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We report the step-by-step process of developing de novo microsatellite (SSR) loci in two Loxosceles spider species. We used reads obtained with next-generation sequencing (Roche 454) to select hundreds of potentially-amplifiable SSRs. After testing amplification and cross-amplification, we characterized 18 SSRs, 11 of which were polymorphic in Loxosceles rufescens (Dufour 1820) and seven of which were polymorphic in L. sp. Fuerteventura -Lanzarote. This method is a relatively fast and economic procedure for the development of fast-evolving nuclear markers in spiders.

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Development of novel microsatellite markers for the spider genusLoxosceles(Sicariidae) using next-generation sequencing

Planas¹,

Bernaus²,

Ribera³

2014

Journal of Arachnology

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Revision of Neotropical wolf spider genus Arctosa C.L. Koch, 1847 (Araneae: Lycosidae), with description of seven new species

PAREDES-MUNGUÍA,

BRESCOVIT,

TEIXEIRA

2024

Zootaxa

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The genus Arctosa C.L. Koch is redescribed and diagnosed. Seven new species are described, four of them based on both sexes (A. conflicta sp. nov., A. costenola sp. nov., A. jibarosa sp. nov. and A. villa sp. nov.), two only on females (A. ayaymama sp. nov. and A. mineira sp. nov.), and one only on the male (A. pacaya sp. nov.). We also propose the transference of Arctosa humicola (Bertkau, 1880) and Arctosa fusca (Keyserling, 1877) to Trochosa C.L. Koch, 1847, and the new synonymy of Arctosa inconspicua (Bryant, 1948) with Trochosa humicola (Bertkau, 1880) comb. nov. Besides, Arctosa andina (Chamberlin, 1916) and Arctosa pugil (Bertkau, 1880) are transferred to Hogna Simon, 1885, and Arctosa aussereri (Keyserling, 1877) to Prolycosides Mello-Leitão, 1942. Additionally, eight lycosid species are synonymized with Prolycosides aussereri: Schizocosa proletaria (Tullgren, 1905); Arctosa workmani (Strand, 1909); Hogna planithoracis (Mello-Leitão, 1938); Hogna variolosa (Mello-Leitão, 1941); Megarctosa melanostoma (Mello-Leitão, 1941); Hippasosa huachoi (Mello-Leitão, 1942); Pirata abalosi (Mello-Leitão, 1942); and Pirata soukupi (Mello-Leitão, 1942). We also transfer Trochosa tenebrosa Keyserling, 1877 to Arctosa. The males of Arctosa tenebrosa (Keyserling, 1877) comb. nov. and Trochosa humicola (Bertkau, 1880) comb. nov. are described for the first time.

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Cost effective microsatellite isolation and genotyping by high throughput sequencing

Krehenwinkel

Meese

Mayer

et al. 2019

The Journal of Arachnology

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Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing

Cited by 3 publications

References 6 publications

Development of novel microsatellite markers for the spider genusLoxosceles(Sicariidae) using next-generation sequencing

Development of novel microsatellite markers for the spider genusLoxosceles(Sicariidae) using next-generation sequencing

Revision of Neotropical wolf spider genus Arctosa C.L. Koch, 1847 (Araneae: Lycosidae), with description of seven new species

Cost effective microsatellite isolation and genotyping by high throughput sequencing

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