Development and Challenge of Fluorescent Probes for Bioimaging Applications: From Visualization to Diagnosis

Triphenylamine-equipped 1,8-naphthaolactam: a versatile scaffold for the custom design of efficient subcellular imaging agents

“…[13][14][15][16][17][18] As the source of emission signals, fluorescence bioprobes are of great importance in this imaging system and very recently have made outstanding contributions to various innovative bioimaging applications. [19][20][21][22][23][24] Given the complex and dynamic features of biological systems, an ideal fluorescent probe in a bioimaging system is demanded to meet the following requirements: (1) bright and constant emission, (2) significant Stokes shifts, (3) excellent biocompatibility and photostability, and (4) easy functional modifiability. The former two primarily allow for high signal-to-noise ratios, while the latter two allow for dynamic and targeted examinations.…”

“…13–18 As the source of emission signals, fluorescence bioprobes are of great importance in this imaging system and very recently have made outstanding contributions to various innovative bioimaging applications. 19–24…”

Fluorescent “AND” logic gates for simultaneous detection of thiols and protons: photophysical properties, mechanism and bioimaging of living cells

“…[29][30][31][32] However, organic small molecules have strengths in biocompatibility, membrane permeability, pharmacokinetic properties, low biotoxicity and fast metabolism. [33][34][35] Therefore, organic small molecules still attract great attention in biological imaging applications. Since the rst molecular logic gate was proposed by de Silver in 1993, 36 many molecular devices have been developed for obtaining information at the molecular-scale.…”

New insight into the application of fluorescence platforms in tumor diagnosis: From chemical basis to clinical application

“…Although the initially developed ultraviolet (UV)‐visible fluorescent probes overcome the inherent defects of clinical diagnostic tools to a certain extent, the problems such as short emission wavelength, large damage to biological samples, weak tissue penetration and poor sensitivity, and so on, the probes are limited from being further applied to in vivo imaging 4 . At the beginning of this century, the exploration in the field of fluorescent probes gradually tilted toward the development of near‐infrared (NIR) probes, and new UV‐visible fluorescent probes such as ratiometric probes and two‐photon probes appeared 5 . In addition, the emergence of concepts such as targeted fluorescent probes, nano‐fluorescent probes, and integrated platforms for diagnosis and treatment and their applications in diagnostics have propelled the field to new heights and achieved unprecedented prosperity in the past 5 years.…”