Development and Carcinogenesis: Roles of GATA Factors in the Sympathoadrenal and Urogenital Systems

Moriguchi, Takashi

doi:10.3390/biomedicines9030299

Cited by 8 publications

(3 citation statements)

References 59 publications

(114 reference statements)

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“…GATA factors, like other transcription factors, function in conjunction with other coregulators, including deacetylases, methyltransferases, and acetyltransferases, to confer specific patterns of gene regulation. 56 GATA3, one of the transcription factors that binds to GATA-containing sequence DNA, targets via two zinc-finger domains. 57 Current evidence indicates that TGF-β negatively regulates GATA3 expression.…”

Section: ■ Discussionmentioning

confidence: 99%

miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation

Han

Gao

Chang

et al. 2022

J. Agric. Food Chem.

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Myostatin (MSTN) is a growth and differentiation factor that regulates proliferation and differentiation of myoblasts, which in turn controls skeletal muscle growth. It may regulate myoblast differentiation by influencing miRNA expression, and the present study aimed to clarify its precise mechanism of action. Here, we found that MSTN −/− pigs showed an overgrowth of skeletal muscle and upregulated miR-455-3p level. Intervention of MSTN expression using siMSTN in C2C12 myoblasts also showed that siMSTN significantly increased the expression of miR-455-3p. It was found that miR-455-3p directly targeted the 3′-untranslated region of Smad2 by dual-luciferase assay. qRT-PCR, Western blotting, and immunofluorescence analyses indicated that miR-455-3p overexpression or Smad2 silencing in C2C12 myoblasts significantly promoted myoblast differentiation. Furthermore, siMSTN significantly increased the expression of GATA3. The levels of miR-455-3p were considerably reduced in C2C12 myoblasts following GATA3 knockdown. Consistently, GATA3 knockdown also reduced the enhanced miR-455-3p expression caused by siMSTN. Finally, we illustrated that GATA3 has a role in myoblast differentiation regulation. Taken together, we identified the expression profiles of miRNAs in MSTN −/− pigs and found that miR-455-3p positively regulates myoblast differentiation. In addition, we revealed that MSTN acts through the GATA3/miR-455-3p/Smad2 cascade to regulate muscle development.

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Section: ■ Discussionmentioning

confidence: 99%

miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation

Han

Gao

Chang

et al. 2022

J. Agric. Food Chem.

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“…Analysis of the action of neuroblastoma PHOX2b variants in developing sympathetic neurons demonstrated their stimulatory effect on neuroblast proliferation combined with dedifferentiation indicating routes to malignancy modulated by PHOX2b in combination with HAND2 (Reiff et al 2010 ). Studies for other transcription factors of the network directing sympathetic neuron differentiation and their role in neuroblastoma exist for GATA3 (Almutairi 2019 ; Moriguchi 2021 ) and ASCL1 (Wang et al 2019 ; Ali et al 2020 ).…”

Section: Domains Of Progress In Understanding the Generation Specification And Connectivity Of Sympathetic Neuron Classesmentioning

confidence: 99%

The sympathies of the body: functional organization and neuronal differentiation in the peripheral sympathetic nervous system

2021

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During the last 30 years, our understanding of the development and diversification of postganglionic sympathetic neurons has dramatically increased. In parallel, the list of target structures has been critically extended from the cardiovascular system and selected glandular structures to metabolically relevant tissues such as white and brown adipose tissue, lymphoid tissues, bone, and bone marrow. A critical question now emerges for the integration of the diverse sympathetic neuron classes into neural circuits specific for these different target tissues to achieve the homeostatic regulation of the physiological ends affected.

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“…Although initially divided into hematopoietic (GATA1/2/3) and cardiac (GATA4/5/6) GATA factors according to their functional characteristics, their functions and expression patterns far exceed these organizations, expressed in various tissues and organs. 5 In addition, several studies have found the aberrant expression of GATA transcription factors in various human malignancies, such as breast cancer, 6,7,8 urinary malignancy, 9 lung cancer, 10 pancreatic cancer. [11][12][13][14][15][16] Chang et al 13 found that GATA1 was highly expressed in pancreatic ductal adenocarcinoma and promoted pancreatic cancer gemcitabine resistance through the anti-apoptotic pathway.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the GATA Transcription Factor Family and Exploration of Their Relevance to Immune Infiltration and Tumor Microenvironment in Pancreatic Cancer

Xu¹,

Cheng²,

Lin³

et al. 2021

IJGM

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Background: Pancreatic cancer (PC) presents a phenomenal disease burden worldwide. The GATA transcription factor family is associated with a variety of human malignancies. However, the relation between GATA family members (GATAs) and PC has not been elucidated. Methods: This study integrates large-scale bioinformatics database resources to analyze the expression patterns of GATAs in PC patients and explore their underlying function mechanism and relevance to immune infiltration and other different cell types in the tumor microenvironment in pancreatic cancer. First, the expression pattern of GATAs in pancreatic cancer was detected by the Oncomine database and the Gene Expression Profile Interaction Analysis (GEPIA2) database and verified through other datasets in the R2 platform. Then, we used the cBioPortal database and the Human Protein Atlas to assess the correlation between GATAs and clinicopathological features of PC. Then, survival analyses were performed to identify candidate prognostic factors in the GATA family in PC patients. Further, we performed gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, protein-protein interaction (PPI) network, immune-infiltration correlation analysis, and cell type analysis of the tumor microenvironment at the single-cell level to explain the function of GATAs in pancreatic cancer. Results: We found that GATA3 and GATA6 were highly expressed in pancreatic cancer, and the expression levels of GATA4 and GATA6 correlated with the pathological stage, differentiation grade, and molecular subtype of pancreatic cancer. The survival analysis revealed that lower GATA4 of PC patients was associated with better outcomes, and higher GATA6 might be associated with longer OS. In addition, GATA3 was associated with immune cell infiltration of PC, and GATA6 was mainly distributed in the epithelial cells with ductal phenotype. Conclusion: This work tentatively identified GATA3, GATA4, and GATA6 in the GATA family associated with pancreatic cancer. GATA4 may serve as a prognostic factor for PC patients, and GATA6 may act as a subtype marker for PC. In addition, GATA3 may reflect the immune-infiltration status of PC.

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Development and Carcinogenesis: Roles of GATA Factors in the Sympathoadrenal and Urogenital Systems

Cited by 8 publications

References 59 publications

miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation

miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation

The sympathies of the body: functional organization and neuronal differentiation in the peripheral sympathetic nervous system

Characterization of the GATA Transcription Factor Family and Exploration of Their Relevance to Immune Infiltration and Tumor Microenvironment in Pancreatic Cancer

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