Development and bioreactor cultivation of a novel semidifferentiated tissue suspension derived from the marine plant Acrosiphonia coalita

Abstract

A semidifferentiated tissue culture consisting of linear filaments in liquid suspension was established from Acrosiphonia coalita, a cold‐water green macroalga known to express pharmacologically active oxylipins deriving from lipoxygenase metabolism of linolenic acid. The tissue was vegatively propagated by blending the filaments down to 1 to 5 mm in length prior to subculture. The filamentous A. coalita tissue suspension was successfully cultivated in an illuminated, 3‐L stirred‐tank bioreactor at 12°C, 0.46‐vvm aeration rate, 250‐rpm mixing speed, and incident illumination intensity of 77 μE m−2s−1. The mean specific growth rate over the exponential phase was 0.185 day−1 and a final cell density of 1083 mg dry cell weight (DCW) L−1 was achieved within 15 days of cultivation from an initial cell density of 200 mg DCW L−1. The addition of 3500 ppm CO2 to the aeration gas provided a maximum CO2 transfer rate of six times the maximum CO2 consumption rate and stabilized the pH to 8.0 during the light phase of growth, but did not improve biomass productivity. © 1996 John Wiley & Sons, Inc.

Suspension culture of gametophytes of transgenic kelp in a photobioreactor

“…Process conditions can then be applied to maximizing biomass production and production of transgenic proteins. As a potentially effective tool, some of bioreactors had been designed for cell and tissue culture of marine macroalgae (Qi & Rorrer 1995, Rorrer & Zhi 1996, Rorrer & Mullikin 1999, Rorrer & Cheney 2004. However, there were no reports of bioreactors culture of cell and tissue of transgenic macroalgae.…”

Study of increasing lipid production from fresh water microalgae Chlorella vulgaris

“…Lower land requirements were also assumed to be possible with the optical fiber devices (Sheehan et al, 1998). These kind of reactors are also able to provide better dark and light photoperiod in the system as evidenced from several experimental works using air lift bioreactor (Novakovic et al, 2005), stirred tank photobioreactor (Huang and Rorrer, 2003) and other types of closed photobioreactor (Rorrer et al, 1996;Rorrer and Mullikin, 1999;Usui and Ikenouchi, 1997;Vernerey et al, 2001). As productivity is measured in terms of biomass produced per day per unit of available surface area, closed photobioreactors will give much higher productivities.…”

Isolation of Halogenated Monoterpenes from Bioreactor-Cultured Microplantlets of the Macrophytic Red Algae Ochtodes secundiramea and Portieria hornemannii

“…Liquid suspensions of O. secundiramea or P. hornemannii microplantlets were cultivated within an externally illuminated, 3 L stirredtank photobioreactor equipped with a three-blade marine impeller of 4.5 cm diameter and 6 cm height. Details on the design and operation of this bioreactor for cultivation of macroalgal tissue suspension cultures are provided by Rorrer et al 35 Typically, the bioreactor vessel was inoculated with 20 g fresh weight of blended, 28 day old flask-cultured tissue along with 2500 mL of sterilized natural seawater/ESS medium using the techniques described above. Conditions of photobioreactor cultivation are given in Table 3.…”