Development and biological validation of a cyclic stretch culture system for the ex vivo engineering of tendons

Raimondi, Manuela Teresa; Laganà, Matteo; Conci, Claudio; Crestani, Michele; Giancamillo, A. Di; Gervaso, Francesca; Deponti, D.; Boschetti, Federica; Nava, Michele M.; Scandone, Chiara; Domeneghini, C.; Sannino, Alessandro; Peretti, Giuseppe M.

doi:10.1177/0391398818774496

Cited by 10 publications

(12 citation statements)

References 36 publications

(47 reference statements)

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“…One way to improve cell-based tendon therapy is to perform a pre-treatment on the cell culture in order to increase cell proliferation and matrix synthesis before an application. Strategies for pre-treatment of tenocyte or MSCs cell culture include mechanical stimulation [8,22], three-dimensional spheroid cultures [23], macromolecular crowding [24] or biochemical supplementation of the culture medium [12,25]. From biochemical supplementation, stimulation by different growth factors, including transforming growth factor-beta (TGF-β) [8], bone morphogenetic protein-12 (BMP-12) [26], connective tissue growth factor (CTGF) [27], and platelet-derived growth factor-BB (PDGF-BB) [28] has been explored.…”

Section: Introductionmentioning

confidence: 99%

Supporting Cell-Based Tendon Therapy: Effect of PDGF-BB and Ascorbic Acid on Rabbit Achilles Tenocytes In Vitro

Evrova

Kellenberger

Calcagni

et al. 2020

IJMS

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Cell-based tendon therapies with tenocytes as a cell source need effective tenocyte in vitro expansion before application for tendinopathies and tendon injuries. Supplementation of tenocyte culture with biomolecules that can boost proliferation and matrix synthesis is one viable option for supporting cell expansion. In this in vitro study, the impacts of ascorbic acid or PDGF-BB supplementation on rabbit Achilles tenocyte culture were studied. Namely, cell proliferation, changes in gene expression of several ECM and tendon markers (collagen I, collagen III, fibronectin, aggrecan, biglycan, decorin, ki67, tenascin-C, tenomodulin, Mohawk, α-SMA, MMP-2, MMP-9, TIMP1, and TIMP2) and ECM deposition (collagen I and fibronectin) were assessed. Ascorbic acid and PDGF-BB enhanced tenocyte proliferation, while ascorbic acid significantly accelerated the deposition of collagen I. Both biomolecules led to different changes in the gene expression profile of the cultured tenocytes, where upregulation of collagen I, Mohawk, decorin, MMP-2, and TIMP-2 was observed with ascorbic acid, while these markers were downregulated by PDGF-BB supplementation. Vice versa, there was an upregulation of fibronectin, biglycan and tenascin-C by PDGF-BB supplementation, while ascorbic acid led to a downregulation of these markers. However, both biomolecules are promising candidates for improving and accelerating the in vitro expansion of tenocytes, which is vital for various tendon tissue engineering approaches or cell-based tendon therapy.

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Section: Introductionmentioning

confidence: 99%

Supporting Cell-Based Tendon Therapy: Effect of PDGF-BB and Ascorbic Acid on Rabbit Achilles Tenocytes In Vitro

Evrova

Kellenberger

Calcagni

et al. 2020

IJMS

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“…Conversely, some authors developed a bioreactor for tendons able to generate an automated cyclic stimulation by applying a manual pre-load, but without a direct feedback or the possibility to modify the strain during the experiments. 16 On the other hand, the OSPB system allows to automatically reach the desired pre-load using a closed-loop feedback control. Moreover, the OSPB software can be pre-programmed to manage and change automatically the stimulation patterns of each chamber during the course and over the time of the experiment.…”

Section: Discussionmentioning

confidence: 99%

Independent, Controllable Stretch-Perfusion Bioreactor Chambers to Functionalize Cell-Seeded Decellularized Tendons

et al. 2019

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“…Actually, the reported strains that could promote tendon differentiation range from 1% to 15% ( Table 1 ). According to the physiological strain of the tendon in vivo , the strain of dynamic stretching should be 4%-8% (at most 10%) [ 60 ]. Zhang et al and Rinoldi et al both applied dynamic stretching with a 15% strain in their studies.…”

Section: Main Strategy Of Mechanical Loading In Tendon Tissue Engimentioning

confidence: 99%

“…The Bose® ElectroForce® BioDynamic® system can monitor sample strain and perform biomechanical tests in real-time [ 62 ]. In addition, various bioreactors are also developed by different research groups to meet their own individual specific requirements, and some of them showed a good performance in constructing engineered tendons ( Figure 2 ) [ 58 , 60 , 63 ].…”

Section: Main Strategy Of Mechanical Loading In Tendon Tissue Engimentioning

confidence: 99%

The Application of Mechanical Stimulations in Tendon Tissue Engineering

Sheng

Jiang

Backman

et al. 2020

Stem Cells International

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Tendon injury is the most common disease in the musculoskeletal system. The current treatment methods have many limitations, such as poor therapeutic effects, functional loss of donor site, and immune rejection. Tendon tissue engineering provides a new treatment strategy for tendon repair and regeneration. In this review, we made a retrospective analysis of applying mechanical stimulation in tendon tissue engineering, and its potential as a direction of development for future clinical treatment strategies. For this purpose, the following topics are discussed; (1) the context of tendon tissue engineering and mechanical stimulation; (2) the applications of various mechanical stimulations in tendon tissue engineering, as well as their inherent mechanisms; (3) the application of magnetic force and the synergy of mechanical and biochemical stimulation. With this, we aim at clarifying some of the main questions that currently exist in the field of tendon tissue engineering and consequently gain new knowledge that may help in the development of future clinical application of tissue engineering in tendon injury.

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Development and biological validation of a cyclic stretch culture system for the ex vivo engineering of tendons

Cited by 10 publications

References 36 publications

Supporting Cell-Based Tendon Therapy: Effect of PDGF-BB and Ascorbic Acid on Rabbit Achilles Tenocytes In Vitro

Supporting Cell-Based Tendon Therapy: Effect of PDGF-BB and Ascorbic Acid on Rabbit Achilles Tenocytes In Vitro

Independent, Controllable Stretch-Perfusion Bioreactor Chambers to Functionalize Cell-Seeded Decellularized Tendons

The Application of Mechanical Stimulations in Tendon Tissue Engineering

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