Development and assessment of stable formulations containing two herbal antimicrobials: <i>Allium sativum L.</i> and <i>Eruca sativa miller</i> seed oils

Sanad, Rania Abdel-Basset; Mabrouk, Mona I.

doi:10.3109/03639045.2015.1096280

Cited by 14 publications

(10 citation statements)

References 61 publications

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“…Haemophilus influenzae MIC 2 µg/mL [65] Staphylococcus aureus MIC 4 µg/mL [65] Streptococcus pneumoniae MIC 8 µg/mL [65] MIC: minimum inhibitory concentration; MID: minimum inhibitory dose; IZ: inhibition zone; O: others hydrophobicity, and viscosity. Their hydrophobic nature worsens the solubility of these compounds in water-based media (e.g., agar, broth), which may reduce their capability for dilution and result in an unequal distribution of active components throughout the medium, as seen in the case of direct contact methods such as broth dilution and disc diffusion tests, even if a proper dispersing or solubilizing agent such as Tween 20/80 or Span 20/80 is used [38,39]. Volatility causes a risk of active substance losses via evaporation during sample handling, experiment preparation, and incubation, depending on time and temperature conditions [40,41].…”

Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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This review summarizes data on the in vitro antimicrobial effectiveness of volatile agents of plant origin and in vitro methods for evaluating their activity in the vapour phase. As a result of literature analysis, the antimicrobial efficacy of vapours from 122 different plant species and 19 pure compounds examined in 61 studies using different in vitro tests against a broad spectrum of microorganisms was identified and summarized. In addition, 11 different techniques found in the literature are described in detail. An original classification of methods based on the solid and liquid matrix volatilization principle is proposed because carrier medium/matrix selection is crucial for the volatilization of any agents tested. This review should be useful for medicinal, pharmaceutical, food, and agricultural experts working in areas related to the management of infectious diseases (especially respiratory and skin infections), food preservation (active packaging), and protection of agriculture products (controlled atmosphere). It may also stimulate the interest of pharmaceutical, cosmetic, food, and agriculture industries in the research and development of new antimicrobial agents of natural origin. Since several original apparatuses previously developed for antimicrobial susceptibility testing in the vapour phase are described in this review, labware manufacturers may also be interested in this topic. The review also provides specific guidelines and recommendations for researchers studying the antimicrobial activity of volatile agents. The article will therefore appeal to communities of industrial stakeholders, pharmacists, physicians, food experts, agriculturists, and researchers in related areas such as pharmacology, medicinal chemistry, microbiology, natural product chemistry, food preservation and plant protection.

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Section: Asteraceae Shoot Disc Volatilization Methodsmentioning

confidence: 99%

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Houdková

Kokoška

2020

Planta Med

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“…Different formulations were prepared using different concentrations of two surface active agents. Cream prepared with 2% surfactant mixture showed well-designed formulation and best antimicrobial activity[108].Taha et al formulated a new herbal antifungal hair cream containing Eruca and garlic oils, active on pathogenic fungi (Malassezia furfur, Microsporum canis Bodin, and Trichophyton mentagiophytes) using different ratios of non-ionic surfactant. Highest permeation rate of alliin in the presence of Eruca oil which is necessary for antifungal activity was obtained with formula of 4% concentration of Span and Brij[109].…”

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confidence: 99%

Eruca Sativa Linn.: Pharmacognostical and Pharmacological Properties and Pharmaceutical Preparations

Jaafar

Jaafar²

2019

Asian J Pharm Clin Res

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Eruca sativa (jarjeer) is an annual herb (family Brassicaceae), which contains a wide range of chemicals and minerals with nutraceutical and organoleptic characteristics. Jarjeer was generally used as a food and traditionally mainly consumed due to its aphrodisiac properties. This crop known to contain various phytochemicals such as flavonoids, phenolic acids, terpens, carotenoids, tannins, glycosides, saponins, sterols, alkaloids, and other secondary metabolites. In leaves, kaempferol and its derivatives, glucosativin, are the main flavonoids and glucosinolate, respectively, while erucic acid and glucoerucin are the main fatty acid and glucosinolate, respectively. Medicinally, the plant has antibacterial, antidiabetic, antihypertensive, antiplatelet, and antioxidant activity and stimulates hair growth and other effects. Trails on topical pharmaceutical preparations involve the use of E. sativa which had been done. These preparations include creams and waxs which are intended to be used for potentiating hair growth and skin fungal and bacterial infection.

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“…Estimulados pelas informações da medicina tradicional e da literatura científica sobre o efeito antimicrobiano do Allium sativum (4)(5)(6)(7)(8)(9)(17)(18)(19)(20) , o presente estudo visou avaliar o efeito sobre cepas de Escherichia coli do extrato bruto do Allium sativum e o efeito da associação medicamentosa deste extrato com os antibióticos Meropenem e Imipenem, pertencentes à classe dos antibióticos carbapenêmicos, empregados no tratamento de infecções por cepas multirresistentes de E. coli.…”

Section: Discussionunclassified

“…Embora estudos anteriores demonstrem o potencial antimicrobiano do alho sobre cepas de E. coli (5,(7)(8)(9)(17)(18)(19)(20) , diferentemente de nosso estudo, nenhum deles fez uso do produto da maceração dos bulbos frescos do alho, o qual após filtração foi empregado, imediatamente, para avaliação da atividade biológica in vitro. Métodos de extração distintos podem determinar respostas biológicas distintas o que determina que a validação do efeito antimicrobiano preceda a investigação de possíveis associações medicamentosas.…”

Section: Discussionunclassified

Avaliação in vitro da associação medicamentosa de extrato bruto de Allium sativum L. (alho) com Imipenem e Meropenem frente a cepas sensíveis de Escherichia coli / In vitro evaluation of drug association between crude Allium sativum L (garlic) extract and Imipenem and Meropenem against sensible Escherichia coli strains

Narimatsu

Lima

Ueda

et al. 2019

Arq Med Hosp Fac Cienc Med Santa Casa São Paulo

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Objetivo: Avaliar a susceptibilidade in vitro de cepas de Escherichia coli ao extrato bruto de Allium sativum e sua associação com antibióticos carbapenêmicos (Meropenem e Imipenem), constatando os impactos dessa associação medicamentosa na inibição do crescimento de cepas de Escherichia coli. Método: Cepas de E. coli obtidas de amostras provenientes do laboratório central do Hospital da Irmandade da Santa Casa de Misericórdia de São Paulo foram semeadas em placas de ágar Müeller-Hinton pelo método de Kirby-Bauer e incubadas por 48 horas. Avaliou-se a suscetibilidade aos antimicrobianos (Meropenem, Imipenem) e ao extrato bruto de A. sativum (EB), líquido obtido por compressão mecânica dos bulbos de alho; e à combinação destes antibióticos associados ao EB nas cepas de E. coli. Aferiram-se os halos de inibição formados segundo os critérios do CLSI vigentes e comparadas as atividades em relação à associação com o EB. Comparou-se o desempenho dos antibióticos isolados, do EB isolado e das associações dos antibióticos com o EB empregando-se o teste estatístico de Wilcoxon. Resultado: Constatou-se desempenho superior das combinações do EB com os discos dos antibióticos (Imipenem e Meromepenem) comparado aos dos discos dos antibióticos isolados e do EB isolado (p<0,05). Conclusão: Concluímos que, in vitro, o Imipenem e o Meropenem apresentaram sinergismo quando associado ao EB de A. sativum frente às cepas testadas.Descritores: Alho, Allium, Escherichia coli, Beta-lactamas, Imipenem, MeropenemAbstract Objective: In vitro evaluation of the susceptibility of E. coli strains to the Allium sativum crude extract, and the impacts of the association between the crude extract of Allium sativum L (garlic) and antibiotics (Meropenem and Imipenem) in the growth inhibition of E. coli strains. Method: E. coli strains were obtained from samples of the laboratory of the Hospital Irmandade da Santa Casa de Misericórdia de São Paulo. The strains were grown on Müeller-Hinton agar disks following the Kirby-Bauer method for 48 hours. The susceptibilities of the strains to the antibiotics (Meropenem and Imipenem); to the A. sativum crude extract (CEA); and to the association of each antibiotic and the CEA were evaluated. The zone diameter of each of these substances was measured following the CLSI criteria. The performance of the isolated antibiotics and of the associations between CEA and the antibiotics were compared by the Wilcoxon test. Results: Against the E. coli strains, the combination of CEA with the antibiotic disk displayed superior performance when compared to the isolated disks of the antibiotics (Imipenem and the Meropenem) and the isolated disk of CEA (p<0,05). Conclusion: Against the tested strains of E. coli, both Meropenem and Imipenem displayed synergism with the CEA.Keywords: Garlic, Allium, Escherichia coli, Beta-lactams, Imipenem, Meropenem

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Development and assessment of stable formulations containing two herbal antimicrobials: Allium sativum L. and Eruca sativa miller seed oils

Abstract: Determination of the rHLB of Garlic and Eruca oils allows the ease of preparation of stable, consistent, and non-irritant cream.

Cited by 14 publications

References 61 publications

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review

Eruca Sativa Linn.: Pharmacognostical and Pharmacological Properties and Pharmaceutical Preparations

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